Development of Origanum vulgare Cell Suspension Culture to Produce Polyphenols and the Stimulation Effect of Salicylic Acid Elicitation and Phenylalanine Feeding

2021 ◽  
Vol 26 (3) ◽  
pp. 456-467
Author(s):  
Yan-Ping Li ◽  
Dao-Bang Tang ◽  
Xiao-Qiang Wang ◽  
Meng Wang ◽  
Qing-Feng Zhang ◽  
...  
Keyword(s):  
Salicylic Acid ◽  
Suspension Culture ◽  
Cell Suspension ◽  
Cell Suspension Culture ◽  
Origanum Vulgare

scholarly journals Ca 2+ ‐dependent and Ca 2+ ‐independent excretion modes of salicylic acid in tobacco cell suspension culture

2001 ◽  
Vol 52 (359) ◽  
pp. 1219-1226
Author(s):  
Hsien‐Jung Chen ◽  
Wen‐Chi Hou ◽  
Joseph Kuć ◽  
Yaw‐Huei Lin
Keyword(s):  
Salicylic Acid ◽  
Suspension Culture ◽  
Cell Suspension ◽  
Cell Suspension Culture ◽  
Tobacco Cell ◽  
Tobacco Cell Suspension

scholarly journals A Study On The Effect of Different Elicitors On Capsaicin Accumulation in Cell Suspension Cultures of Capsicum Assamicum (Bhut Jolokia)

2021 ◽  
Author(s):  
Swet Nisha ◽  
Ajitabh Bora ◽  
HK Gogoi ◽  
SK Dwivedi ◽  
PJ Handique
Keyword(s):  
Salicylic Acid ◽  
Suspension Culture ◽  
Methyl Jasmonate ◽  
Cell Suspension ◽  
Cell Suspension Culture ◽  
Sinapic Acid ◽  
Cell Suspension Cultures ◽  
Suspension Cultures ◽  
Exponential Phase ◽  
Bhut Jolokia

Abstract Elicitation of cell suspension cultures of Capsicum assamicum (Bhut Jolokia) for enhancement of capsaicin content was tried using different elicitors such as cellulase, vanillin, methyl jasmonate, salicylic acid and sinapic acid in different concentrations for 24, 48 and 72 hours. Cell suspension culture was established in B5 media supplemented with 3.5 mM 2,4-D (2,4-diphenoxyacetic acid) and 1.1 mM Kin and elicitors were introduced at the end of exponential phase. All the elicitors, except methyl jasmonate, led to significant increase in production of capsaicin. Sinapic acid, when added in 22 µM concentration and incubated for 24 hours, led to highest capsaicin accumulation of 0.5% (5068 µg/g) which was highest among all the treatments.

scholarly journals Ca2+‐dependent and Ca2+‐independent excretion modes of salicylic acid in tobacco cell suspension culture

2001 ◽  
Vol 52 (359) ◽  
pp. 1219-1226 ◽  
Author(s):  
Hsien‐Jung Chen ◽  
Wen‐Chi Hou ◽  
Joseph Kuć ◽  
Yaw‐Huei Lin
Keyword(s):  
Salicylic Acid ◽  
Suspension Culture ◽  
Cell Suspension ◽  
Cell Suspension Culture ◽  
Tobacco Cell ◽  
Tobacco Cell Suspension

scholarly journals Influence of plant growth regulators and salicylic acid on the production of some secondary metabolites in callus and cell suspension culture of Satureja sahendica Bornm.

2021 ◽  
Vol 117 (4) ◽  
pp. 1
Author(s):  
Sarieh TARIGHOLIZADEH ◽  
Rouhollah MOTAFAKKERAZAD ◽  
Morteza KOSARI-NASAB ◽  
Ali MOVAFEGHI ◽  
Sakineh MOHAMMADI ◽  
...  
Keyword(s):  
Salicylic Acid ◽  
Secondary Metabolites ◽  
Plant Growth ◽  
Suspension Culture ◽  
Cell Suspension ◽  
Plant Growth Regulators ◽  
Growth Regulators ◽  
Cell Suspension Culture ◽  
Total Phenolics ◽  
Fresh Mass

<p class="042abstractstekst">The impact of combinations of plant growth regulators (PGRs) on callus culture of <em>Satureja sahendica </em>Bornm. was investigated. In nodal explants, the response of secondary metabolite production to different concentrations of PGRs was analyzed regarding the presence and absence of polyvinylpyrrolidone (PVP). The explants were cultured on MS media in presence of auxins (2,4-dichlorophenoxyacetic acid and naphthylacetic acid) and cytokinins (thidiazuron and kinetin); which were used in equal concentrations of 0.5, 1, and 2 mg l<sup>-1</sup>. The treatment of 2 mg l<sup>-1</sup> 2,4-D + 2 mg l<sup>-1 </sup>Kin (MD3) led to the highest production of total phenolics (4.303 ± 0.449 mg GAE g<sup>-1</sup>) and flavonoids (24.903 ± 7.016 mg QE g<sup>-1</sup>). Moreover, the effect of salicylic acid (SA) on the production of secondary metabolites in cell suspension culture of <em>Satureja sahendica</em> was evaluated. The cell suspension culture was established by culturing the nodal-derived friable callus in the liquid medium containing different concentrations of SA (0, 100, 150, 200 µM). An inverse relationship exists between the fresh mass and secondary metabolites contents. In addition, there was a significant difference among concentrations of SA in the production of total phenolics and flavonoid compounds. SA enhances secondary metabolites production and decreases cell fresh mass.</p>

Effect of Salicylic acid on Phenols and flavonoids content and DPPH scavenging activity in cell suspension culture of Iranian sodab (Ruta graveolens)

2020 ◽  
Author(s):  
Yaser Sharifi ◽  
Valiollah Ghasemi Omran ◽  
Toktam Sadat Tavabe Ghavami ◽  
Ali Nematzadeh Gharakhili ◽  
Mohammad Ali Ebrahimzadeh
Keyword(s):  
Salicylic Acid ◽  
Suspension Culture ◽  
Cell Suspension ◽  
Cell Suspension Culture ◽  
Radical Scavenging ◽  
Total Phenolic ◽  
Scavenging Activity ◽  
Ruta Graveolens ◽  
Dpph Scavenging Activity ◽  
Dpph Scavenging

Ruta graveolens, locally called sodab or sadab, is a known medicinal plant in north of Iran. The plant is a source for the production of secondary metabolites such as Glycosides, lignins, alkaloids, Coumarins, flavonoids and phenolic acids. In this work, the effect of salicylic acid on phenols and flavonoids contents of R. graveolens were investigated in the cell suspension culture. A significant enhancement in total phenolic and flavonoids contents were observed in salicylic acid treated samples. The results revealed that salicylic acid (10 mg/ml) showed as maximum as 3.14 fold enhancement in total flavonoid content (40.35 mg/g) and salicylic acid (20 mg quercetin equivalent/g of extract powder) showed as maximum as 18.33 fold improvement in total phenolic content (438.75 mg gallic acid equivalent/g of extract powder). Also in this study, salicylic acid (10 mg/ml) showed as maximum as 3.55 fold increase in DPPH radical-scavenging activity compared to control. These results suggest that the positive effect of salicylic acid on phenols and flavonoids content and DPPH scavenging activity in cell suspension culture. This effect was dose dependent.

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