scholarly journals Influence of plant growth regulators and salicylic acid on the production of some secondary metabolites in callus and cell suspension culture of Satureja sahendica Bornm.

2021 ◽  
Vol 117 (4) ◽  
pp. 1
Author(s):  
Sarieh TARIGHOLIZADEH ◽  
Rouhollah MOTAFAKKERAZAD ◽  
Morteza KOSARI-NASAB ◽  
Ali MOVAFEGHI ◽  
Sakineh MOHAMMADI ◽  
...  
Keyword(s):  
Salicylic Acid ◽  
Secondary Metabolites ◽  
Plant Growth ◽  
Suspension Culture ◽  
Cell Suspension ◽  
Plant Growth Regulators ◽  
Growth Regulators ◽  
Cell Suspension Culture ◽  
Total Phenolics ◽  
Fresh Mass

<p class="042abstractstekst">The impact of combinations of plant growth regulators (PGRs) on callus culture of <em>Satureja sahendica </em>Bornm. was investigated. In nodal explants, the response of secondary metabolite production to different concentrations of PGRs was analyzed regarding the presence and absence of polyvinylpyrrolidone (PVP). The explants were cultured on MS media in presence of auxins (2,4-dichlorophenoxyacetic acid and naphthylacetic acid) and cytokinins (thidiazuron and kinetin); which were used in equal concentrations of 0.5, 1, and 2 mg l<sup>-1</sup>. The treatment of 2 mg l<sup>-1</sup> 2,4-D + 2 mg l<sup>-1 </sup>Kin (MD3) led to the highest production of total phenolics (4.303 ± 0.449 mg GAE g<sup>-1</sup>) and flavonoids (24.903 ± 7.016 mg QE g<sup>-1</sup>). Moreover, the effect of salicylic acid (SA) on the production of secondary metabolites in cell suspension culture of <em>Satureja sahendica</em> was evaluated. The cell suspension culture was established by culturing the nodal-derived friable callus in the liquid medium containing different concentrations of SA (0, 100, 150, 200 µM). An inverse relationship exists between the fresh mass and secondary metabolites contents. In addition, there was a significant difference among concentrations of SA in the production of total phenolics and flavonoid compounds. SA enhances secondary metabolites production and decreases cell fresh mass.</p>

scholarly journals Elicitation of Bacoside Content Using Plant Growth Regulators in Cell Suspension Culture of Bacopa monnieri (L.) Wettst.

2018 ◽  
Vol 28 (2) ◽  
pp. 191-199 ◽  
Author(s):  
AV Kharde ◽  
SV Kore ◽  
MB Khetmalas
Keyword(s):  
Plant Growth ◽  
Suspension Culture ◽  
Cell Suspension ◽  
Plant Growth Regulators ◽  
Growth Regulators ◽  
Cell Suspension Culture ◽  
Casein Hydrolysate ◽  
Leaf Explants ◽  
Bacopa Monnieri ◽  
Naphthalene Acetic Acid

Cell suspension culture is used for the optimization of plant growth regulators used for the elicitation of bacoside content of Bacopa monnieri (L.) Wettst. Callus induction was carried out on MS supplemented with 2, 4-D (0.5 mg/l) by using leaf explants. For suspension culture MS liquid medium was supplemented with constant α-naphthalene acetic acid (0.5 mg/l) and casein hydrolysate (1 g/l) as well as the varied concentrations of 6-benzyleadenine, kinetin, thidiazuaron and chitosan. It was observed that bacoside production was increased by 6- benzyleadenine (0.5 mg/l), Kn (0.5 mg/l) and thidiazuaron (0.25 mg/l) was 3.07, 3.79 and 3.97 mg/g, respectively. This indicates that lower concentration of growth regulator favor the bacoside enhancement under in vitro conditions, while chitosan affects the bacoside production in suspension culture. In this study thidiazuaron at 0.25 mg/l induces maximum elicitation of bacoside content i.e. 3.97 mg/g. Plant Tissue Cult. & Biotech. 28(2): 191-199, 2018 (December)

scholarly journals Improvement of the chilling tolerance of ‘Monika’ cucumber seedlings by short-term temperature and seed conditioning with plant growth regulators

2014 ◽  
Vol 26 (2) ◽  
pp. 109-115
Author(s):  
Krzysztof Górnik
Keyword(s):  
Salicylic Acid ◽  
Plant Growth ◽  
Jasmonic Acid ◽  
Dehydrogenase Activity ◽  
Plant Growth Regulators ◽  
Growth Regulators ◽  
Chilling Tolerance ◽  
Short Term ◽  
Cucumber Seedlings ◽  
Seed Conditioning

ABSTRACT The aim of the present study was to improve the chilling tolerance of cucumber seedlings. The conditioned seeds in solutions of salicylic or jasmonic acid in concentrations of 10-2, 10-3 or 10-4 M or brassinolide in concentrations of 10-6, 10-8 or 10-10 M were subjected to temperature shock of 0, 2.5, 5, 35, 40 or 45°C for 1, 2 or 4 hours. Seedlings with 3 mm roots were chilled at 0°C for three days. The chilling susceptibility was evaluated by measurements of roots lengths, electrolyte leakage and total dehydrogenase activity. The obtained results indicated that cucumber tolerance to chilling conditions depended on the plant growth regulators used during seed imbibition and its concentration as well as the temperature initiating thermal shock and its duration. The highest tolerance to chilling, expressed by the subsequent growth of roots, was observed after seed conditioning in salicylic acid solutions in a concentration of 10-4 M for 16 h at 25°C, then exposed for 4 h to a shock temperature of 5°C. The length of seedling roots after such treatment was over 12 times longer than the control (imbibed in distilled water and not subjected to short-term temperature impact). An increased chilling tolerance of cucumber seedlings may result from enhanced membrane integrity and total dehydrogenase activity. Further research is needed to explain the mechanism of the positive effects of salicylic acid, jasmonic acid or brassinolide application to reduce the injurious effects of chilling on cucumber seedlings.

scholarly journals Influence of Plant Growth Regulators and Micronutrients on Growth, Yield and Quality of Sorghum under Temperate Conditions

2021 ◽  
pp. 18-23
Author(s):  
Zahida Rashid ◽  
Tanveer Ahmad Ahngar ◽  
B. Sabiya ◽  
N. Sabina ◽  
N. S. Khuroo ◽  
...  
Keyword(s):  
Salicylic Acid ◽  
Plant Growth ◽  
Plant Growth Regulators ◽  
Growth Regulators ◽  
Foliar Spray ◽  
Growth Yield ◽  
Soil Application ◽  
Yield And Quality ◽  
Green Fodder ◽  
Fodder Yield

A field experiment was conducted at Dry land Agricultural Research Station, Rangreth, Srinagar, SKUAST-K in Kharif 2020 to study the effect of Plant Growth Regulators and micronutrients on growth, yield and quality of sorghum. The objective of the study was to assess the effect of Plant Growth Regulators and micronutrients on herbage yield and quality. The treatments included; T1: Tricontanol 10 ppm at 30 DAS (foliar spray), T2: Salicylic acid 100 ppm at 30 DAS (foliar spray), T3: 5 kg Zn/ha soil application, T4: 2 kg B/ha soil application, T5: 5 kg Zn + 2 kg B/ha soil application, T6: 5 kg Zn/ha (soil application ) + Triacontanol 10 ppm at 30 DAS (foliar spray), T7: 5 kg Zn/ha (soil application) + salicylic acid 100 ppm at 30 DAS (foliar spray), T8: 2 kg B/ha (soil application) + Triacontanol 10 ppm at 30 DAS (foliar spray), T9: 2 kg B/ha (soil application )+ salicylic acid 100 ppm at 30 DAS (foliar spray), T10: 5 kg Zn + 2 kg B/ha (soil application) + Triacontanol 10 ppm at 30 DAS (foliar spray), T11: 5 kg Zn + 2 kg B/ha (soil application) + salicylic acid 100 ppm at 30 DAS (foliar spray) and T12: Water spray at the time of PGR application. Zn and B were applied at the time of sowing in the soil. The crop was raised with recommended package of practices. In treatments, where zinc was not a treatment, an amount of sulphur through gypsum equivalent to sulphate supplied with 5 kg ZnSO4 was applied to compensate. The crop was sown in 30.0 cm apart lines. The trial was laid out in Randomized Block Design with three replications. The results indicated that all the treatments improved the green fodder yield over control. Among different treatments, T10: 5 kg Zn + 2 kg B/ha soil application + Triacontanol 10 ppm at 30 DAS foliar spray and T11: 5 kg Zn + 2 kg B/ha soil application + salicylic acid 100 ppm at 30 DAS foliar spray produced maximum GFY (493.6 and 490.5q/ha) on locational mean basis. It was significantly superior to other treatments. These treatments improved the green fodder yields by 35.0 % and 34.2 %, respectively, over control (spray of water). In terms of dry matter, similar trend was noted and the improvement with T10 and T11 was to the tune of 36.8 % and 41.0 % over control. Tricontanol 10 ppm at 30 DAS (foliar spray) (T1) improved the green fodder yield and dry fodder yield by 13.6% and 14.3 % respectively over T12Water spray at the time of Plant Growth Regulator application. Similarly spray of T2: Salicylic acid 100 ppm at 30 DAS (foliar spray) improved the green fodder yield and dry fodder yield by 14.4% and 15.4% respectively over T12Water spray at the time of Plant Growth Regulator application. Similar trend was observed with respect to quality parameters (crude protein content and crude protein yield) of sorghum.

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