Successful Localization of Abnormal Parathyroid Gland Using Ultrasound-Guided Methylene Blue Dye Injection in the Reoperative Neck

Background: Breast cancer has the highest incidence and mortality among female malignant tumors. Breast cancer with negative axillary lymph nodes has been diag- nosed mainly at an early stage. Sentinel lymph node biopsy (SLNB) is a standard screening technique for patients with early-stage breast cancer and clinically nega- tive lymph nodes. Lymphoscintigraphy (sentinel lymph node mapping) has been reg- ularly used as the standard method for SLNB. Today, ultrasound-guided wire locali- zation (USGWL) is a well-established technique with superior outcomes. Therefore, we attempted to determine whether preoperative UGWL and lymphoscintigraphy (blue dye and isotope injection) improve SLN detection and false-negative rate in breast cancer patients undergoing SLNB and identify clinical factors that may affect the diagnostic accuracy of axillary ultrasound (AUS). Methods: Between December 2018 and June 2019, 55 patients with clinical T1- 3N0 breast cancer eligible for an SLNB at Imam Khomeini Hospital in Tehran were included in our study. Tumor characteristics and demographic data were collect- ed by reviewing medical records and questionnaires prepared by our surgical team. The day before SLNB, all patients underwent ultrasound-guided wire localization of SLN. Lymphoscintigraphy was performed with an unfiltered 99mTc-labelled sulfur colloid peritumoral injection followed by methylene blue dye injection. The results were analyzed based on the permanent pathology report. Results: Among the 55 patients, 71.8% of SLNs were detected by wire localization, while 57.8% were found by methylene blue mapping and 59.6% by gamma probe detection. Compared with wire localization and isotope injection, the methylene blue dye technique had a low sensitivity (72.2%), while both wire localization and isotope injection reached 77.8%. The sensitivity, specificity, and accuracy of UGWL were 77.8%, 42.1%, and 65.4%, respectively. Otherwise, methylene blue dye and isotope injection accuracy was 47.3% and 50.1%, respectively. Furthermore, there was a significant relationship between BMI, tumor size, laterality, reactive ALN, and the accuracy of preoperative AUS. But there was no significant correlation between age, weight, height, tumor biopsy, tumor location, the time interval between methylene blue dye and isotope injection to surgery, and also the type of surgery to the accuracy of preoperative AUS. Conclusion: Preoperative UGWL can effectively identify SLNs compared to lym- phoscintigraphy (blue dye and isotope injection) in early breast cancer patients un- dergoing SLNB.

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Successful Localization of Recurrent Thyroid Cancer in Reoperative Neck Surgery Using Ultrasound-Guided Methylene Blue Dye Injection

Journal of the American College of Surgeons ◽

10.1016/j.jamcollsurg.2012.06.006 ◽

2012 ◽

Vol 215 (4) ◽

pp. 555-561 ◽

Cited By ~ 28

Author(s):

Avital Harari ◽

Rebecca S. Sippel ◽

Ruth Goldstein ◽

Seerat Aziz ◽

Wen Shen ◽

...

Keyword(s):

Methylene Blue ◽

Thyroid Cancer ◽

Neck Surgery ◽

Blue Dye ◽

Methylene Blue Dye ◽

Ultrasound Guided ◽

Recurrent Thyroid Cancer

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Ultrasound-Guided Inferior Alveolar Nerve Block in the Horse: Assessment of the Extraoral Approach in Cadavers

Journal of Veterinary Dentistry ◽

10.1177/0898756419844836 ◽

2019 ◽

Vol 36 (1) ◽

pp. 46-51

Author(s):

Jessica Purefoy Johnson ◽

Robert Karl Peckham ◽

Conor Rowan ◽

Alan Wolfe ◽

John Mark O’Leary

Keyword(s):

Methylene Blue ◽

Nerve Block ◽

Inferior Alveolar Nerve ◽

Lingual Nerve ◽

Blue Dye ◽

Methylene Blue Dye ◽

Inferior Alveolar Nerve Block ◽

Success Rates ◽

Ultrasound Guided ◽

Dye Staining

Blinded techniques to desensitize the inferior alveolar nerve (IAN) include intraoral, angled, and vertical extraoral approaches with reported success rates of 100%, 73%, and 59%, respectively. It has not been determined whether an ultrasound-guided extraoral approach is feasible. Further, the fascicular nature of the inferior alveolar and lingual nerves of the horse has not been described. The objectives of this study were to describe a low-volume ultrasound-guided vertical extraoral inferior alveolar nerve block technique and to describe the fascicular nature of these nerves. An ultrasound-guided approach to the IAN was conducted with a microconvex transducer and an 18-G, 15-cm spinal needle using a solution containing iodinated-contrast and methylene blue dye. Accuracy was assessed by contrast visualized at the mandibular foramen on computed tomography (CT) and methylene blue dye staining of the nerves on gross dissection. Sections of inferior alveolar and lingual nerves were submitted for histological analysis. Assessment by CT and dissection determined success rates of 81.3% and 68.8%, respectively; 68.8% of injections had inadvertent methylene blue dye staining of the lingual nerve. Nerve histology revealed both the inferior alveolar and lingual nerves to be multifascicular in nature. Mean fascicle counts for the inferior alveolar and lingual nerves were 29 and 30.8, respectively. The technique is challenging and no more accurate than previously published blinded techniques. Any extraoral approach to the IAN is likely to also desensitize the lingual nerve.

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Proximal Perineural Femoral Nerve Injection in Pigs Using an Ultrasound-Guided Lateral Subiliac Approach—A Cadaveric Study

Animals ◽

10.3390/ani11061759 ◽

2021 ◽

Vol 11 (6) ◽

pp. 1759

Author(s):

Robert Trujanovic ◽

Pablo E. Otero ◽

Peter Marhofer ◽

Ulrike Auer ◽

Silvio Kau

Keyword(s):

Methylene Blue ◽

Pain Control ◽

Femoral Nerve ◽

Blue Dye ◽

Methylene Blue Dye ◽

Ultrasound Guided ◽

Technical Feasibility ◽

Pelvic Limb ◽

Psoas Major

Desensitizing the femoral nerve (FN) improves pain control in several species undergoing pelvic limb surgeries. Despite its advantages, this method has not yet been described in pigs, although they make an accepted surgical animal model. Based on anatomical dissections, first performed in two pig cadavers, an ultrasound-guided access for localization and perineural infiltration of the FN trunk at the iliopsoas compartment level was specified. The FN was found running between the psoas major and medial portion of iliac muscle. Ultrasonographically, the FN appeared as a hypoechogenic round–oval structure surrounded by a hyperechogenic rim. Technical feasibility and accuracy were assessed in six additional pig cadavers by injecting 0.15 mL kg−1 methylene blue dye bilaterally in direct proximity to the nerve. The needle was inserted caudoventral to the coxal tuber, traversing the ultrasound plane as it progressed towards the FN in dorsomedial direction. Staining of the nerve was evaluated by dissection. The injection was considered effective if the nerve was stained in its entire circumference over a length of 2 cm. FNs of all investigated individuals could be successfully stained. This ultrasound-guided subiliac approach allows feasible and accurate access to the FN and may be useful in producing a successful blockade in vivo.

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Using of modified-bentonite as low-cost sorbent for removal of methylene blue dye from aqueous solution

Association of Arab Universities Journal of Engineering Sciences ◽

10.33261/jaaru.2020.27.2.005 ◽

2020 ◽

Vol 27 (2) ◽

pp. 45-54

Author(s):

Saraa Muwafaq Ibrahim ◽

Ziad T. Abd Ali

Keyword(s):

Aqueous Solution ◽

Methylene Blue ◽

Silanol Group ◽

Infrared Spectrometry ◽

Blue Dye ◽

Ammonium Bromide ◽

Vibration Band ◽

Order Kinetic ◽

Methylene Blue Dye ◽

Modified Bentonite

Batch experiments have been studied to remove methylene blue dye (MB) from aqueous solution using modified bentonite. The modified bentonite was synthesized by replacing exchangeable calcium cations in natural bentonite with cationic surfactant cetyl trimethyl ammonium bromide (CTAB). The characteristics of modified bentonite were studied using different analysis such as Scanning electronic microscopy (SEM), Fourier transform infrared spectrometry (FTIR) and surface area. Where SEM shows the natural bentonite has a porous structure, a rough and uneven appearance with scattered and different block structure sizes, while the modified bentonite surface morphology was smooth and supplemented by a limited number of holes. On other hand, (FTIR) analysis that proved NH group aliphatic and aromatic group of MB and silanol group are responsible for the sorption of contaminate. The organic matter peaks at 2848 and 2930 cm-1 in the spectra of modified bentonite which are sharper than those of the natural bentonite were assigned to the CH2 scissor vibration band and the symmetrical CH3 stretching absorption band, respectively, also the 2930 cm-1 peak is assigned to CH stretching band. The batch study was provided the maximum removal efficiency (99.99 % MB) with a sorption capacity of 129.87 mg/g at specified conditions (100 mg/L, 25℃, pH 11 and 250rpm). The sorption isotherm data fitted well with the Freundlich isotherm model. The kinetic studies were revealed that the sorption follows a pseudo-second-order kinetic model which indicates chemisorption between sorbent and sorbate molecules.

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