Occurrence of ochratoxin A in spices commercialized in Abidjan (Côte d’Ivoire)

2016 ◽  
Vol 32 (3) ◽  
pp. 137-143 ◽  
Author(s):  
Pierre Manda ◽  
Ketty Michele Adanou ◽  
Dembelé Ardjouma ◽  
Aholia Jean Baptiste Adepo ◽  
Djédjé Sébastien Dano
2006 ◽  
Vol 23 (10) ◽  
pp. 1000-1007 ◽  
Author(s):  
Béatrice Sangare-Tigori ◽  
Serge Moukha ◽  
H. James Kouadio ◽  
Anne-Marie Betbeder ◽  
Djédjé Sébastien Dano ◽  
...  

Author(s):  
Ehouman Ano Guy Serge ◽  
Yao N’Zue Benjamin ◽  
Hampoh Ade Hortense ◽  
Fofana Alimata ◽  
Traore Karim Sory ◽  
...  

Author(s):  
Kouamé Désiré ◽  
Niamketchi Gilles Léonce ◽  
Konan Constant ◽  
Konan Ysidor ◽  
Biego G. Henri Marius

Aims: Fungal pathogens are one of the main biological agents causing maize post-harvest loss and affect food security in the country. Thus, this study was conducted to assess fungal pathogens associated to post-harvest maize (Zea mays L.) with especial focus to mycotoxin-producing fungi at producer’s storage condition in different regions of Côte d’Ivoire. Study Design: A total of 1 500 samples of maize as grains, cobs and husks were collected at rate of 500 samples by region (Gbêkê, Poro, Hambol, Indénié-Djuablin and Gontougo) and sent to the laboratory in order to analyse their sanitary quality. Place and Duration of Study: This study was carried out during March 2016 to January 2017. The analyses of the collected sample carried out at the Biotechnology, Agriculture and Valorisation of Biological Resources Laboratory of the Félix Houphouët-Boigny’s University, Abidjan. Methodology: Microbiological analysis was assessed by recording the number of colony in the plate. The contents of aflatoxin and ochratoxin A in the different samples were determined using standard methods. Results: The total microbial species isolated ranged from 104 to 1011 cfu/g with thermotolerants coliforms (103 – 104) and fecal coliforms (102 – 103). The stored maize samples contained also high amount of yeast and molds (104 to 107 cfu/g). Aspergillus genus was the predominantly fungal isolated in all maize samples with tree species which are A. flavus, A. niger and A. versicolor potential producers of mycotoxins. More importantly, stored maize sample as grain, cobs and husks were affected by aflatoxins (B1, B2, G1 and G2) and ochratoxin A. Sixty per cent of the maize samples, mostly husks, showed aflatoxin B1 (from 12.73 to 130.31 µg/kg) and OTA (from 16.75 to 134.21 µg/kg) concentrations above the Maximum Authorized Limit of 5 µg/kg. Conclusion: A significant variability from one region to another can be noticed at level of maize quality regardless the type of maize. The sanitary quality of maize seems to be tied to postharvest treatments (drying), type of storage (grains, cobs and husks) and structure of storage.


2006 ◽  
Vol 25 (4) ◽  
pp. 211-216 ◽  
Author(s):  
B Sangare-Tigori ◽  
A A Dem ◽  
H J Kouadio ◽  
A-M Betbeder ◽  
D S Dano ◽  
...  

In a preliminary study, samples of millet (n=33) maize (n=41), rice (n=10) and peanuts (n=10) from Côte d'Ivoire were analysed for ochratoxin A (OTA) by HPLC with fluorimetric detection, followed by confirmation by cleavage of the OTA molecule using carboxypeptidase with HPLC separation and fluorimetric quantification of the released ochratoxin alpha (OTα). With the exception of four samples of peanuts, all samples showed OTA contamination, ranging from 3 to 1738 μg/kg. All cereals were contaminated and the OTA concentrations were in the range of 17-204 μg/kg for millet, 3-1738 μg/kg for maize, 9-92 μg/kg for rice and 0.6-64 μg/kg for peanuts, depending on the year of harvest. Most of the samples would not be accepted according to the EU regulatory limits for this mycotoxin. Following this survey, research for other mycotoxins and the evaluation of the exposure of the population is under-way.


Toxicon ◽  
2006 ◽  
Vol 47 (8) ◽  
pp. 894-900 ◽  
Author(s):  
Béatrice Sangare-Tigori ◽  
Serge Moukha ◽  
James H. Kouadio ◽  
Djédjé Sébastien Dano ◽  
Anne-Marie Betbeder ◽  
...  

Author(s):  
Sira Bamba ◽  
Henri Marius Godi Biego ◽  
Assi Yolande Ake ◽  
Adama Coulibaly

Aims : The aim of this work is to take stock of the level of ochratoxin A (OTA), fumonisin B1 (FB1) and zearalenone (ZEA) contamination in maize produced in Cote d'Ivoire in order to help improve its quality. Study Design: Maize samples (375) were taken in five producing regions (Poro, Hambol, Gontougo, Gbêkê, Indénié-Djuablin). Place and Duration of Study: the collection was carried out on maize in grain, on the cob and in spathe from February 2016 to January 2017. Then, the analyzes were carried out at the Biotechnology Laboratory, Agriculture and Development of Biological Resources of the Félix HOUPHOUËT-BOIGNY University. Methodology: The determination of ochratoxin A, fumonisin B1 and zearalenone was carried out according to the methods of regulation No. 401/2006/EC, AFNOR, Miraglia and Brera. Results: The results indicate the presence of ochratoxin A, fumonisin B1 and zearalenone in all forms of maize (grains, cob, spathes) and the five regions visited. However, the average concentrations of fumonisin B1 and zearalenone are respectively 27.46 µg/kg-1999.22 µg/kg and 8.48 µg/kg-341.84 µg/kg and are lower than the prescribed reference standards (2000 µg/kg ; 500 µg/kg). For ochratoxin A, the average concentrations vary from 0.83 µg/kg to 14.38 µg/kg ; 1.92 µg/kg to 18.60 µg/kg and 2.21 µg/kg to 134.89 µg/kg respectively for grains, cob and spathes. Samples from the Regions of Poro, Gbêkê and Hambol have mean concentrations below the maximum reference limit of 5µg/kg. Thus, variability in the sanitary quality of maize was demonstrated from one region to another, regardless of the form of the maize. Based on the principal component analysis, spathes represent the form of maize most prone to high contamination regardless of mycotoxin and région. Conclusion: The searching alternative storage methods and the right form of maize storage could be a solution to the high mycotoxin contamination of marketed maize.


2019 ◽  
Vol 13 (4) ◽  
pp. 369-374
Author(s):  
A. Yao ◽  
A. Hué ◽  
J. Danho ◽  
P. Koffi-Dago ◽  
M. Sanogo ◽  
...  

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