Molecular Detection of Human Enteric Adenoviruses in Water Samples Collected from Lake Victoria Waters Along Homa Bay Town, Homa Bay County, Kenya

2020 ◽  
Author(s):  
Wasonga Michael Opere ◽  
Maingi John ◽  
Omwoyo Ombori
Keyword(s):  
Water Samples ◽  
Molecular Detection ◽  
Lake Victoria ◽  
Enteric Adenoviruses

Detection of enteric adenoviruses in south African waters using gene probes

1995 ◽  
Vol 31 (5-6) ◽  
pp. 345-350 ◽  
Author(s):  
B. Genthe ◽  
M. Gericke ◽  
B. Bateman ◽  
N. Mjoli ◽  
R. Kfir
Keyword(s):  
South African ◽  
Water Samples ◽  
Cellulose Fibre ◽  
Gene Probe ◽  
Viral Dna ◽  
Virus Particles ◽  
Gene Probes ◽  
Probe Hybridization ◽  
Enteric Adenoviruses ◽  
Effect Of Chlorine

Gene probes developed locally for both enteric Adenoviruses 40 and 41 were used to determine whether these viruses were present in both raw and treated waters. Approximately sixty water samples were concentrated by ultrafiltration and analysed directly for the presence of enteric adenoviruses. Three pretreatment techniques, namely sephadex columns, cellulose fibre and GenecleanTM were tested for the removal of inhibitory substances from concentrated water samples. The effect of chlorine treatment on viral detection using gene probe hybridization was also examined by exposing adenoviruses to chlorine concentrations of up to 20mg/l for 1 hour. Enteric adenoviruses were detected in up to 59% of both raw and treated waters analysed. Cellulose fibre and GenecleanTM were found to successfully remove inhibitory substances from concentrated raw waters. Viral DNA was detected after exposure to a range of chlorine concentrations indicating that the viruses detected in the treated waters may have been inactivated virus particles.

scholarly journals Molecular Detection of Tick-Borne Pathogen Diversities in Ticks from Livestock and Reptiles along the Shores and Adjacent Islands of Lake Victoria and Lake Baringo, Kenya

2017 ◽  
Vol 4 ◽  
Author(s):  
David Omondi ◽  
Daniel K. Masiga ◽  
Burtram C. Fielding ◽  
Edward Kariuki ◽  
Yvonne Ukamaka Ajamma ◽  
...  
Keyword(s):  
Molecular Detection ◽  
Lake Victoria

Organochlorine Residues in Fish and Water Samples from Lake Victoria, Uganda

2006 ◽  
Vol 35 (2) ◽  
pp. 584-589 ◽  
Author(s):  
G. N. Kasozi ◽  
B. T. Kiremire ◽  
F. W. B. Bugenyi ◽  
N. H. Kirsch ◽  
P. Nkedi-Kizza
Keyword(s):  
Water Samples ◽  
Lake Victoria

Quantitative detection of human enteric adenoviruses in river water by microfluidic digital polymerase chain reaction

2014 ◽  
Vol 70 (3) ◽  
pp. 555-560 ◽  
Author(s):  
Naohiro Kishida ◽  
Naohiro Noda ◽  
Eiji Haramoto ◽  
Mamoru Kawaharasaki ◽  
Michihiro Akiba ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Real Time ◽  
River Water ◽  
Water Samples ◽  
Real Time Pcr ◽  
Quantitative Detection ◽  
Chain Reaction ◽  
Polymerase Chain ◽  
Digital Polymerase Chain Reaction ◽  
Enteric Adenoviruses

We describe an assay for simple and accurate quantification of human enteric adenoviruses (EAdVs) in water samples using a recently developed quantification method named microfluidic digital polymerase chain reaction (dPCR). The assay is based on automatic distribution of reaction mixture into a large number of nanolitre-volume reaction chambers and absolute copy number quantification from the number of chambers containing amplification products on the basis of Poisson statistics. This assay allows absolute quantification of target genes without the use of standard DNA. Concentrations of EAdVs in Japanese river water samples were successfully quantified by the developed dPCR assay. The EAdVs were detected in seven of the 10 samples (1 L each), and the concentration ranged from 420 to 2,700 copies/L. The quantified values closely resemble those by most probable number (MPN)-PCR and real-time PCR when standard DNA was validated by dPCR whereas they varied substantially when the standard was not validated. Accuracy and sensitivity of the dPCR was higher than those of real-time PCR and MPN-PCR. To our knowledge, this is the first study that has successfully quantified enteric viruses in river water using dPCR. This method will contribute to better understanding of existence of viruses in water.

scholarly journals First description of Adenovirus, Enterovirus, Rotavirus and Torque teno virus in water samples collected from the Arroio Dilúvio, Porto Alegre, Brazil

2012 ◽  
Vol 72 (2) ◽  
pp. 323-329 ◽  
Author(s):  
AD Vecchia ◽  
JD Fleck ◽  
J Comerlato ◽  
M Kluge ◽  
B Bergamaschi ◽  
...  
Keyword(s):  
Water Samples ◽  
Detection Rate ◽  
Molecular Detection ◽  
Torque Teno Virus ◽  
Large Urban Area ◽  
Porto Alegre ◽  
Viral Genomes ◽  
Treated Sewage ◽  
Area Sampling ◽  
The City

Adenovirus (AdV), enterovirus (EV), genogroup A rotaviruses (GARV) and Torque teno virus (TTV) are non-enveloped viral agents excreted in feces and so may contaminate water bodies. In the present study, the molecular detection of these viruses was performed in samples of surface water collected from the Arroio Dilúvio, a waterstream that crosses the city of Porto Alegre, RS, Brazil, receiving great volumes of non-treated sewage from a large urban area. Sampling was performed during 2009, in three different occasions (January, April and September). The highest detection rate was observed for EV (64.28%), followed by TTV (28.57%) and AdV (21.43%). Rotaviruses were not detected. More than on kind of tested virus was detected in five (35. 71%) of 14 samples. January was the month with the highest viral detection rate, being all samples, collected in this month, positive for at least one group of tested virus. The correlation between the detection of these different viral agents and environmental factors is discussed. To the knowledge of the authors, this is the first description of viral genomes in water samples taken from the Arroio Dilúvio, Porto Alegre (Brazil).

scholarly journals Adenovirus, enterovirus and thermotolerant coliforms in recreational waters from Lake Guaíba beaches, Porto Alegre, Brazil

2015 ◽  
Vol 13 (4) ◽  
pp. 1123-1129 ◽  
Author(s):  
C. P. Maurer ◽  
A. B. Simonetti ◽  
R. Staggemeier ◽  
C. Rigotto ◽  
L. S. Heinzelmann ◽  
...  
Keyword(s):  
Water Samples ◽  
Molecular Detection ◽  
Water Safety ◽  
Porto Alegre ◽  
Recreational Waters ◽  
Government Regulations ◽  
Viral Genomes ◽  
Low Concentrations ◽  
Thermotolerant Coliforms ◽  
Surface Water Samples

In the present study, molecular detection of human adenoviruses (HAdV) and enteroviruses (EV) was performed in surface water samples collected from beaches Ipanema and Lami, located on the shores of Lake Guaíba, city of Porto Alegre, RS, southern Brazil. Furthermore, water safety was evaluated by counting thermotolerant coliforms (TC), following local government regulations. A total of 36 samples were collected monthly from six different sites along the beaches. Viral genomes were found in 30 (83.3%) samples. The higher detection rate was observed for HAdV (77.8%), followed by EV (22.2%). Although low concentrations of TC have been found, the occurrence of viral genomes in water samples was frequent and may pose a potential risk of infection for people bathing in these beaches.

Molecular detection and genotypic characterization of enteric adenoviruses in a hospital wastewater

2018 ◽  
Vol 25 (11) ◽  
pp. 10977-10987 ◽  
Author(s):  
Chourouk Ibrahim ◽  
Abdennaceur Hassen ◽  
Pierre Pothier ◽  
Selma Mejri ◽  
Salah Hammami
Keyword(s):  
Molecular Detection ◽  
Hospital Wastewater ◽  
Genotypic Characterization ◽  
Enteric Adenoviruses
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