scholarly journals Detection of Leishmania major DNA within wild caught Phlebotomus papatasi and species composition of sand flies in endemic focus of cutaneous leishmaniasis, in western Iran

2014 ◽  
Vol 40 (1) ◽  
pp. 69-74 ◽  
Author(s):  
A. Vahabi ◽  
Y. Rassi ◽  
M. A. Oshaghi ◽  
M. Sayyadi ◽  
S. Rafizadeh
Keyword(s):  
Species Composition ◽  
Cutaneous Leishmaniasis ◽  
Leishmania Major ◽  
Sand Flies ◽  
Phlebotomus Papatasi ◽  
Western Iran ◽  
Endemic Focus

scholarly journals Species composition of sand flies and bionomics of Phlebotomus papatasi and P. sergenti (Diptera: Psychodidae) in cutaneous leishmaniasis endemic foci, Morocco

2016 ◽  
Vol 9 (1) ◽  
Author(s):  
Samia Boussaa ◽  
Kholoud Kahime ◽  
Abdallah M. Samy ◽  
Abdelkrim Ben Salem ◽  
Ali Boumezzough
Keyword(s):  
Species Composition ◽  
Cutaneous Leishmaniasis ◽  
Sand Flies ◽  
Phlebotomus Papatasi

scholarly journals Ecology of sandflies (Diptera: Psychodidae) in a restricted focus of cutaneous leishmaniasis in Northern Venezuela: II. Species composition in relation to habitat, catching method and hour of catching

1987 ◽  
Vol 82 (1) ◽  
pp. 125-131 ◽  
Author(s):  
M. Dora Feliciangeli
Keyword(s):  
Species Composition ◽  
Cutaneous Leishmaniasis ◽  
Term Study ◽  
Endemic Focus ◽  
Phlebotomine Sandflies ◽  
The Common ◽  
Catching Methods

The ecology of phlebotomine sandflies in an endemic focus of cutaneous leishmaniasis in Northern Venezuela (San Esteban, Carabobo State) was investigated through a year-term study. Three different habitats: viz. a house, a pridomestic area and a sylvatic area, were covered and the species composition, the abundance and occurrence of each species were analyzed in relation to the habitats, catching methods and hour of catching. L. panamensis, L. gomezi and L. ovallesi are the species which bite man, although almost exclusively at night. All of them hide by day and are common in the sylvatic area. Moreover, L. panamensis and L. gomezi successfully approach the house and seem to settle in the peridomestic area. L. shannoni and L. olmeca bicolor also approach and accidentally bite man. L. trinidadensis, L. atroclavata and L. cayennensis are the common non-antrhopophilic species in the area.

Relationship Between the Distribution and Biodiversity of Sand Flies (Diptera: Psychodidae) With the Incidence of Zoonotic Cutaneous Leishmaniasis in Endemic Foci of Golestan Province, Iran

2020 ◽  
Vol 57 (6) ◽  
pp. 1768-1774
Author(s):  
Aioub Sofizadeh ◽  
Kamran Akbarzadeh ◽  
Ehsan Allah Kalteh ◽  
Fatemeh Karimi
Keyword(s):  
Cutaneous Leishmaniasis ◽  
Diversity Index ◽  
Wiener Index ◽  
Sand Flies ◽  
Phlebotomus Papatasi ◽  
Light Traps ◽  
Phlebotomine Sand Flies ◽  
Zoonotic Cutaneous Leishmaniasis ◽  
Golestan Province ◽  
The Relationship

Abstract Zoonotic cutaneous leishmaniasis (ZCL) is prevalent in Golestan Province, Iran. The current study determined the relationship between the distribution and biodiversity of sand flies with cutaneous leishmaniasis at 14 villages in plain and hillsides areas. In each village from July to September 2017, 60 sticky traps and 2 CDC light traps were laid. Spearman and Mann–Whitney tests were used to determine the relationship between the incidence of ZCL and the abundance of different species of sand flies. Simpson, Shannon-Wiener, Evenness, and Margalef indices were calculated to estimate the diversity of species. A total of 5,295 phlebotomine sand flies were collected, comprising 10 species of the genus Phlebotomus (3,947 flies) and 7 species of genus Sergentomyia (1,248 flies). The abundance of sand flies and incidence of ZCL in plain areas were greater than that of hillsides areas (P = 0.013, P = 0.002). There was a significant correlation between the incidence of ZCL and the abundance of Phlebotomus papatasi (r = 0.72, P = 0.004) and P. caucasicus groups (P = 0.006; 0.022). In the Shannon-Wiener index, the rest of the biodiversity indices were reduced in higher-altitude areas. Increasing Shannon-Wiener index showed higher diversity of sand flies in higher-altitude areas. Data of the reported cases of leishmaniasis in plain areas can reveal the relationship between less diversity index (Shannon-Wiener), higher dominant diversity index (Simpson), and incidence of leishmaniasis in these areas.

Baseline susceptibility of a wild strain of Phlebotomus papatasi (Diptera: Psychodidae) to DDT and pyrethroids in an endemic focus of zoonotic cutaneous leishmaniasis in Iran

2012 ◽  
Vol 68 (5) ◽  
pp. 669-675 ◽  
Author(s):  
Zahra Saeidi ◽  
Hassan Vatandoost ◽  
Amir Ahmad Akhavan ◽  
Mohamad Reza Yaghoobi-Ershadi ◽  
Yavar Rassi ◽  
...  
Keyword(s):  
Cutaneous Leishmaniasis ◽  
Wild Strain ◽  
Phlebotomus Papatasi ◽  
Endemic Focus ◽  
Zoonotic Cutaneous Leishmaniasis

scholarly journals Epidemiological study in a new focus of cutaneous leishmaniasis in the Islamic Republic of Iran

2003 ◽  
Vol 9 (4) ◽  
pp. 816-826
Author(s):  
M. R. Yaghoobi Ershadi ◽  
A. A. Akhavan ◽  
A. V. Zahraei Ramazani ◽  
M. R. Abai ◽  
B. Ebrahimi ◽  
...  
Keyword(s):  
Cutaneous Leishmaniasis ◽  
Epidemiological Study ◽  
Leishmania Major ◽  
Reservoir Host ◽  
Age Group ◽  
Phlebotomus Papatasi ◽  
Islamic Republic ◽  
Islamic Republic Of Iran ◽  
Rhombomys Opimus

An outbreak of cutaneous leishmaniasis [CL] in Sabzevar county prompted this study of the epidemiology and the ecology of vectors and reservoirs. Examination of 541 schoolchildren showed rates of 9.4% for scars and 5.9% for ulcers. Among 807 inhabitants of 4 villages, 10.4% had scars and 3.0% had active lesions. The most highly infected age group was 0-4 years with a rate of 5.9%. A total of 12 849 sandflies representing 7 species were collected in the study area. Leptomonad infection was found in Phlebotomus papatasi, P. caucasicus and Sergentomyia sintoni. Parasites from man, P. papatasi and Rhombomys opimus, were isolated and characterized as Leishmania major. Based on this survey, this is an epidemic of zoonotic CL, with R. opimus the main reservoir host, and P. papatasi the main vector

Some Ecological Aspects of Phlebotominae Sand Flies (Diptera: Psychodidae) in an Endemic Focus of Anthroponotic Cutaneous Leishmaniasis of Iran

2007 ◽  
Vol 5 (1) ◽  
pp. 17-23 ◽  
Author(s):  
Ali R. Zahraei-Ra ◽  
Mohammad R. Yaghoobi-E ◽  
Amir A. Akhavan ◽  
Hamid Abdoli ◽  
Reza Jafari ◽  
...  
Keyword(s):  
Cutaneous Leishmaniasis ◽  
Sand Flies ◽  
Endemic Focus ◽  
Ecological Aspects

scholarly journals CRISPR/Cas9 Mutagenesis in Phlebotomus papatasi: the Immune Deficiency Pathway Impacts Vector Competence for Leishmania major

mBio ◽  
2019 ◽  
Vol 10 (4) ◽  
Author(s):  
Isabelle Louradour ◽  
Kashinath Ghosh ◽  
Ehud Inbar ◽  
David L. Sacks
Keyword(s):  
Immune Deficiency ◽  
Immune Response ◽  
Leishmania Major ◽  
Vector Competence ◽  
Targeted Mutagenesis ◽  
Sand Fly ◽  
Sand Flies ◽  
Phlebotomus Papatasi ◽  
Content Type ◽  
Imd Pathway

ABSTRACT Sand flies are the natural vectors for the Leishmania species that produce a spectrum of diseases in their mammalian hosts, including humans. Studies of sand fly/Leishmania interactions have been limited by the absence of genome editing techniques applicable to these insects. In this report, we adapted CRISPR (clustered regularly interspaced palindromic repeat)/Cas9 (CRISPR-associated protein 9) technology to the Phlebotomus papatasi sand fly, a natural vector for Leishmania major, targeting the sand fly immune deficiency (IMD) pathway in order to decipher its contribution to vector competence. We established a protocol for transformation in P. papatasi and were able to generate transmissible null mutant alleles for Relish (Rel), the only transcription factor of the IMD pathway. While the maintenance of a homozygous mutant stock was severely compromised, we were able to establish in an early generation their greater susceptibility to infection with L. major. Flies carrying different heterozygous mutant alleles variably displayed a more permissive phenotype, presenting higher loads of parasites or greater numbers of infective-stage promastigotes. Together, our data show (i) the successful adaptation of the CRISPR/Cas9 technology to sand flies and (ii) the impact of the sand fly immune response on vector competence for Leishmania parasites. IMPORTANCE Sand flies are the natural vectors of Leishmania parasites. Studies of sand fly/Leishmania interactions have been limited by the lack of successful genomic manipulation of these insects. This paper shows the first example of successful targeted mutagenesis in sand flies via adaptation of the CRISPR/Cas9 editing technique. We generated transmissible null mutant alleles of relish, a gene known to be essential for the control of immune response in other insects. In addition to the expected higher level of susceptibility to bacteria, the mutant flies presented higher loads of parasites when infected with L. major, showing that the sand fly immune response impacts its vector competence for this pathogen.

Sign in / Sign up

Export Citation Format

Share Document