Nutrient Removal from Dairy and Poultry Wastewater with Simultaneous Biomass and Biodiesel Production by Chlorella sp. T4 Isolated from a Freshwater Stream in South Africa

2021 ◽  
Author(s):  
S’fiso Thuthukani Gumbi ◽  
Taurai Mutanda ◽  
Ademola O. Olaniran
Keyword(s):  
South Africa ◽  
Nutrient Removal ◽  
Biodiesel Production ◽  
Chlorella Sp ◽  
Freshwater Stream ◽  
Poultry Wastewater

scholarly journals Exploration of Microalgal Species for Nutrient Removal from Anaerobically Digested Swine Wastewater and Potential Lipids Production

2021 ◽  
Vol 9 (12) ◽  
pp. 2469
Author(s):  
Zhihui Chen ◽  
Yunhua Xiao ◽  
Tan Liu ◽  
Mingmin Yuan ◽  
Gang Liu ◽  
...  
Keyword(s):  
Nutrient Removal ◽  
Lipid Production ◽  
Ammonia Nitrogen ◽  
Biodiesel Production ◽  
Swine Wastewater ◽  
Biomass Composition ◽  
Chlorella Sp ◽  
Growth Performances ◽  
Lipids Production ◽  
Nitrogen Phosphorus

Bio-treatment of anaerobically treated swine wastewater (ADSW) mediated by microalgae has been deemed as a promising strategy. In the present study, six microalgal strains were used to conduct batch experiments in 0~100% ADSW in order to evaluate their potentials for nutrient removal and biodiesel production. Two strains, Chlorella vulgaris FACHB-8 and Chlorella sp. FACHB-31, were selected based on their better growth performances, higher tolerance to wastewater (up to 100%), and better nutrient removal abilities. The capacity of each strain to remove TN, TP, NH4+-N, as well as lipid production and biomass composition in 100% ADSW were further examined. After 15 days of culture, 87.68~89.85%, 92.61~93.68%, and 97.02~97.86% of the nitrogen, phosphorus, and ammonia nitrogen were removed by Chlorella sp. FACHB-31 and C. vulgaris FACHB-8. Their lipid content and lipid productivities were 29.63~33.33% and 18.91~23.10 mg L−1 d−1, respectively. Proteins were both the major biomass fraction followed by lipids and then carbohydrates. Their fatty acid profiles both mainly consisted of C-16:0, C-18:1, C-18:0, and C-18:2. Taken together, our results suggest that C. vulgaris FACHB-8 and Chlorella sp. FACHB-31 are potential candidates for biodiesel production by using ADSW as a good feedstock.

scholarly journals COMPARATIVE STUDIES OF CELL GROWTH OF FRESHWATER MICROALGA CHLORELLA SP. IN PHOTOAUTOTROPHIC, HETEROTROPHIC AND MIXOTROPHIC CULTURES

2016 ◽  
Vol 78 (7) ◽  
Author(s):  
Costantine Joannes ◽  
Rachel Fran Mansa ◽  
Suhaimi Md. Yasir ◽  
Jedol Dayou
Keyword(s):  
Cell Growth ◽  
Cell Concentration ◽  
Alternative Pathway ◽  
Biodiesel Production ◽  
Microalgae Cultivation ◽  
Heterotrophic Culture ◽  
Mixotrophic Culture ◽  
Chlorella Sp ◽  
Carbon Compounds ◽  
Culture Mode

Lately, research on biodiesel production as a renewable and sustainable energy has become increasingly apparent due to the fact that fossil fuel is decreasing and the concern of global warming issues. The third generation of biofuel, which is microalgae-based biodiesel had gained interest over the last decade. The ability of microalgae to grow in various conditions is one of its advantages as the potential and promising feedstock for biodiesel. Microalgae can be cultivated in three modes such as photoautotrophic, heterotrophic and mixotrophic culture mode. Unlike photoautotrophic mode where light is required, the heterotrophic mode mainly utilized carbon compounds to grow. On the other hand, the mixotrophic mode is the condition where light and carbon compounds are supplied for microalgae culturing. This paper investigates the cell growth of Chlorella sp. cultivated in photoautotrophic, heterotrophic and mixotrophic culture mode. It was found that Chlorella sp. was capable of producing the highest cell concentration of 6.67 ± 0.56 x 106 cell mL-1 in the photoautotrophic mode for 23 days of cultivation period. This was 1.3 times and 3.2 times greater than the cell concentration in mixotrophic (5.02 ± 0.49 x 106 cell mL-1) and heterotrophic (2.03 ± 0.29 x 106 cell mL-1) culture, respectively. On the contrary, the highest specific growth rate obtained in the study was from heterotrophic mode (0.32 ± 0.04 day-1) followed by photoautotrophic and mixotrophic mode with 0.26 ± 0.05 day-1 and 0.20 ± 0.04 day-1, respectively. Chlorella sp. cell grew well under the photoautotrophic and mixotrophic mode. However, the insufficient of glucose level had contributed to lower cells productivity in the heterotrophic culture. Therefore, the mixotrophic mode could also be an alternative pathway in microalgae cultivation for biodiesel production if the glucose supplied was adequate and at the suitable level.  

Utilization of de-oiled algal biomass for enhancing vehicular quality biodiesel production from Chlorella sp. in mixotrophic cultivation systems

2018 ◽  
Vol 122 ◽  
pp. 80-88 ◽  
Author(s):  
Richa Katiyar ◽  
Randhir K. Bharti ◽  
B.R. Gurjar ◽  
Amit Kumar ◽  
Shalini Biswas ◽  
...  
Keyword(s):  
Algal Biomass ◽  
Biodiesel Production ◽  
Mixotrophic Cultivation ◽  
Cultivation Systems ◽  
Chlorella Sp

scholarly journals Statistical Approach of Nutrient Optimization for Microalgae Cultivation

2020 ◽  
Vol 141 ◽  
pp. 03009
Author(s):  
Pichayatorn Bunkaew ◽  
Sasithorn Kongruang
Keyword(s):  
Light Intensity ◽  
Cobalt Chloride ◽  
Lipid Production ◽  
Biodiesel Production ◽  
Mixotrophic Cultivation ◽  
Microalgae Cultivation ◽  
Cultivation Time ◽  
Chlorella Sp ◽  
Chloride Hexahydrate ◽  
Cobalt Chloride Hexahydrate

The Plackett-Burman Design (PBD) was applied to study fresh water microalgae cultivation using Chlorella sp. TISTR 8411 to select the influential nutrient factors for biomass and lipid production. The PBD for 13 trials from 11 nutrient factors with 3 levels was studied in the mixotrophic cultivation at 28 0C under 16:8 light and dark photoperiods over 7 days of cultivation time. Two influential factors were chosen as glucose and cobalt chloride hexahydrate to further design via Box-Behnken Design (BBD) in order to optimize the cultivation of this microalgae for biodiesel production. The 17 trials of 3 factors and 3 levels of BBD experimental design technique were applied with varying factors of glucose (20-40 g/L), cobalt chloride hexahydrate (0.01-0.04 mg/L) and light intensity (4,500-7,500 Lux) under 16:8 light and dark photoperiods over 7 days of cultivation time at 28 0C. Result showed that Chlorella sp. TISTR 8411 cultivation yield 0.52 g/L biomass and 0.31 g/L lipid production resulting in approximately 60% of lipid production when cultivated in 20.05 g/L glucose, 0.04 mg/L CoCl26H2O under light intensity of 4,614 Lux with the supplementation of 4.38 g/L NaHCO3 coupled with 1 g/L of both NaNO3 and KH2PO4. Under statically mixotrophic cultivation, result indicated that Chlorella sp. TISTR 8411 had potential to produce high lipid content for biodiesel application and biomass production for nutraceutical application. Further experiment with the longer cultivation period up to 2 weeks would implement not only for monitoring the growth kinetics but also evaluating the suitable type of fatty acid production.

Sign in / Sign up

Export Citation Format

Share Document