Protective role of lipoic acid on methotrexate induced intestinal damage in rabbit model

The study was aimed at evaluating the protective role of α-lipoic acid (ALA) on long-term exposure of rats to the combination of chlorpyrifos (CPF) and deltamethrin (DLT). Forty-two (42) male Wistar rats were divided into 6 exposure groups with 7 animals in each group: (I) soya oil (2 ml kg−1), (II) ALA (60 mg kg−1), (III) DLT (6.25 mg kg−1), (IV) CPF (4.75 mg kg−1), (V) (CPF + DLT) DLT (6.25 mg kg−1) and CPF (4.75 mg kg−1; 1/20th of the previously determined median lethal dose) and (VI) (ALA + CPF + DLT) pretreated with ALA (60 mg kg−1) and then co-exposed to CPF and DLT, 45 min later. The regimens were administered by gavage once daily for a period of 16 weeks. Sera obtained from blood collected at the end of the experimental period were used for the evaluation of serum glucose, total protein, albumin, urea, creatinine and the activities of alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase, lactate dehydrogenase and acetylcholinesterase. The liver homogenate was used to assay for the activities of superoxide dismutase and glutathione peroxidase and the concentrations of malondialdehyde, cytokine and tumour necrotic factor α. The result showed that the combination of CPF and DLT resulted in marked alterations of these biochemical parameters in most cases compared to either of the pesticides singly, supplementation with ALA ameliorated these alterations.

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The Protective Role of Prenatal Alpha Lipoic Acid Supplementation against Pancreatic Oxidative Damage in Offspring of Valproic Acid-Treated Rats: Histological and Molecular Study

Biology ◽

10.3390/biology9090239 ◽

2020 ◽

Vol 9 (9) ◽

pp. 239

Author(s):

Fatma M. Ghoneim ◽

Hani Alrefai ◽

Ayman Z. Elsamanoudy ◽

Salwa M. Abo El-khair ◽

Hanaa A. Khalaf

Keyword(s):

Oxidative Stress ◽

Oxidative Damage ◽

Lipoic Acid ◽

Caspase 3 ◽

Protective Role ◽

Alpha Lipoic Acid ◽

Group Iii ◽

Rat Offspring ◽

Group I

Background: Sodium valproate (VPA) is an antiepileptic drug (AED) licensed for epilepsy and used during pregnancy in various indications. Alpha-lipoic acid (ALA) is a natural compound inducing endogenous antioxidant production. Our study aimed to investigate the effect of prenatal administration of VPA on the pancreas of rat offspring and assess the potential protective role of ALA co-administration during pregnancy. Methods: Twenty-eight pregnant female albino rats were divided into four groups: group I (negative control), group II (positive control, ALA treated), group III (VPA-treated), and group IV (VPA-ALA-treated). The pancreases of the rat offspring were removed at the fourth week postpartum and prepared for histological, immune-histochemical, morphometric, molecular, and oxidative stress marker studies. Results: In group III, there were pyknotic nuclei, vacuolated cytoplasm with ballooning of acinar, α, and β cells of the pancreas. Ultrastructural degeneration of cytoplasmic organelles was detected. Additionally, there was a significant increase in oxidative stress, a decrease in insulin-positive cell percentage, and an increase in glucagon positive cells in comparison to control groups. Moreover, VPA increased the gene expression of an apoptotic marker, caspase-3, with a decrease in anti-apoptotic Bcl2 and nuclear factor erythroid 2-related factor 2 (Nrf2) transcriptional factor. Conversely, ALA improved oxidative stress and apoptosis in group VI, and a consequent improvement of the histological and ultrastructure picture was detected. Conclusion: ALA co-administration with VPA significantly improved the oxidative stress condition, histological and morphometric picture of the pancreas, and restored normal expression of related genes, including Nrf2, caspase-3, and Bcl-2. Administration of α-lipoic acid has a protective effect against VPA-induced pancreatic oxidative damage via its cytoprotective antioxidant effect.

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Protective role of alpha-lipoic acid in impairments of social and stereotyped behaviors induced by early postnatal administration of thimerosal in male rat

Neurotoxicology and Teratology ◽

10.1016/j.ntt.2018.02.002 ◽

2018 ◽

Vol 67 ◽

pp. 1-9 ◽

Cited By ~ 5

Author(s):

Zahra Namvarpour ◽

Mohammad Nasehi ◽

Abdollah Amini ◽

Mohammad-Reza Zarrindast

Keyword(s):

Lipoic Acid ◽

Protective Role ◽

Male Rat ◽

Alpha Lipoic Acid ◽

Stereotyped Behaviors

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The Protective Role of Alpha Lipoic Acid Against pesticides Induced Testicular Toxicity- Histopathological and Histochemical Studies

Journal of Aquaculture Research & Development ◽

10.4172/2155-9546.1000101 ◽

2010 ◽

Vol 01 (01) ◽

Cited By ~ 4

Author(s):

Azza M. Gawish

Keyword(s):

Lipoic Acid ◽

Protective Role ◽

Testicular Toxicity ◽

Alpha Lipoic Acid

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Investigating the Possible Protective Role of Direct Intra-arterial Administration of Mannitol and N-Acetylcysteine and Per Os Administration of Simvastatin Against Contrast-Induced Nephropathy: An Experimental Study in a Rabbit Model

CardioVascular and Interventional Radiology ◽

10.1007/s00270-019-02304-8 ◽

2019 ◽

Vol 42 (12) ◽

pp. 1777-1785 ◽

Cited By ~ 1

Author(s):

Thomas E. Kalogirou ◽

Soultana Meditskou ◽

Sotiria Davidopoulou ◽

Ioannis Savvas ◽

Apostolos G. Pitoulias ◽

...

Keyword(s):

Experimental Study ◽

Rabbit Model ◽

Protective Role ◽

Contrast Induced Nephropathy

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Protective role of DLa-lipoic acid against adriamycin-induced cardiac lipid peroxidation

Human & Experimental Toxicology ◽

10.1191/0960327103ht358oa ◽

2003 ◽

Vol 22 (5) ◽

pp. 249-254 ◽

Cited By ~ 13

Author(s):

A V Balachandar ◽

K P Malarkodi ◽

P Varalakshmi

Keyword(s):

Lipid Peroxidation ◽

Free Radicals ◽

Lipoic Acid ◽

Antioxidant Property ◽

Protective Role ◽

Cytoprotective Activity ◽

Male Wistar Rats ◽

Cellular Defence ◽

Antitumour Drug

The cytoprotective activity of a-lipoic acid against free radical toxicity manifested during adriamycin (ADR)induced cardiotoxicity has been investigated. ADR is a potent antitumour drug known to cause severe cardiotoxicity. Although ADR generates free radicals, the role of these radicals in the development of cardiac toxicity is still not well understood. In the present study, we evaluated the influence of chronic ADR treatment on the cellular defence mechanism against free radicals and the effect of a-lipoic acid supplementation on ADR-induced cardiotoxicity in male Wistar rats. The increase in lipid peroxidation (LPO) and activities of serum myocardial enzymes, namely lactate dehydrogenase (LDH) and creatinephosphokinase, associated with the decrease in activities of enzymatic (SOD, CAT, GPx, G6PD and GR) and non-enzymatic (GSH, Vit C and Vit E) antioxidants levels were the salient features observed in ADR-induced cardiotoxicity. Lipoic acid pretreated groups showed significant increase in activities of both enzymatic and non-enzymatic antioxidant levels. These observations highlight the antioxidant property ofa-lipoic acid and its cytoprotective action against ADR-induced cardiotoxicity.

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