The Removal of Antibiotics in Relation to a Microbial Community in an Integrated Constructed Wetland for Tail Water Decontamination

Constructed-wetlands, Biofilms, and sedimentation are potential aquaculture tail-water treatments however their roles on the distribution of benthic microbial community and the way they affect the interaction between microbial community and inorganic nutrient fluxes have not been fully explored. This study applied 16S rRNA high-throughput sequencing technology to investigate the microbial community distribution and their link with nutrient fluxes in an aquaculture tail- water bioremediation system . Results showed that bacterial community compositions were significantly different in constructed-wetland and biofilm treatments (p<0.05) relative to sedimentation. The composition of the 16S rRNA genes among all the treatments was enriched with Proteobacteria, Bacteroidetes, Firmicutes, and Flavobacteria . NMDS analysis showed that the bacterial composition in constructed-wetland and biofilm samples clustered separately compared to those in sedimentation. The Functional-Annotation-of-Prokaryotic-Taxa analysis indicated that the proportions of sediment-microbial-functional groups (aerobic-chemoheterophy, chemoheterotrophy, and nitrate-ammonification combined) in the constructed-wetland treatment were 47%, 32% in biofilm and 13% in sedimentation system. Benthic-nutrient fluxes for phosphate, ammonium, nitrite, nitrate and sediment oxygen consumption differed markedly among the treatments ( p<0.05 ). Canonical correspondence analysis indicated constructed-wetland had the strongest association between biogeochemical contents and the bacterial community relative to other treatments. This study suggests that the microbial community distributions and their interactions nutrient fluxes were most improved in the constructed-wetland followed by the area under biofilm and sedimentation treatment.

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Correlating microbial community with physicochemical indices and structures of a full-scale integrated constructed wetland system

Applied Microbiology and Biotechnology ◽

10.1007/s00253-016-7526-4 ◽

2016 ◽

Vol 100 (15) ◽

pp. 6917-6926 ◽

Cited By ~ 24

Author(s):

Yinghai Wu ◽

Rui Han ◽

Xunan Yang ◽

Xiaohang Fang ◽

Xi Chen ◽

...

Keyword(s):

Microbial Community ◽

Constructed Wetland ◽

Full Scale ◽

Integrated Constructed Wetland ◽

Wetland System

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Seasonal variation of microbial community for the treatment of tail water in constructed wetland

Water Science & Technology ◽

10.2166/wst.2017.124 ◽

2017 ◽

Vol 75 (10) ◽

pp. 2434-2442 ◽

Cited By ~ 6

Author(s):

Kang Liang ◽

Yanran Dai ◽

Feihua Wang ◽

Wei Liang

Keyword(s):

Microbial Community ◽

Removal Efficiency ◽

Constructed Wetland ◽

Oxygen Demand ◽

Illumina Platform ◽

Treatment Performance ◽

N Removal ◽

Significant Difference ◽

Tail Water ◽

Low Nitrogen

Effects of seasons and hydraulic loading rates (HLR) on the treatment performance and the response of the microbial community of vertical flow constructed wetland treating tail water were investigated. The seasonal treatment performance was evaluated at four HLR of 125, 250, 375 and 500 mm/d, respectively. The microbial community was detected by MiSeq Illumina platform at HLR 125 and 375 mm/d. The wetland showed significantly higher chemical oxygen demand (COD) and total nitrogen (TN), total phosphorus (TP) at HLR 125 mm/d, compared with other HLR. Overall removal efficiency was 61.47%, 71.40% and 76.31% for COD, TN and TP, respectively, while no significant differences for COD, TN and TP removal were found at HLR of 250, 375 and 500 mm/d. The best removal efficiency for COD and TN was achieved in summer and autumn, while the best TP removal was achieved in winter. Nitrification bacteria (Nitrosomonas and Nitrospira) were significantly higher in HLR 125 mm/d, whereas sequences associated with denitrification had no significant difference at the two HLR. The results can partially explain the significantly higher NH4+-N removal in HLR 125 mm/d and relatively low nitrogen performance in winter.

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