No mutual symbiosis following infection of algae-free Paramecium bursaria with symbiotic algae from Mayorella viridis

Symbiosis ◽  
2017 ◽  
Vol 75 (1) ◽  
pp. 51-59
Author(s):  
Shion Kawai ◽  
Sotaro Araki ◽  
Yuuki Kodama
Keyword(s):  
Paramecium Bursaria ◽  
Symbiotic Algae

Genetic Evidence of "American" and "European" Type Symbiotic Algae of Paramecium bursaria Ehrenberg

Plant Biology ◽  
2005 ◽  
Vol 7 (5) ◽  
pp. 526-532 ◽  
Author(s):  
R. Hoshina ◽  
Y. Kato ◽  
S. Kamako ◽  
N. Imamura
Keyword(s):  
Genetic Evidence ◽  
Paramecium Bursaria ◽  
Symbiotic Algae

Micro-Particle Transporting System Using Galvanotactically Stimulated Apo-Symbiotic Cells of Paramecium bursaria

2009 ◽  
Vol 64 (5-6) ◽  
pp. 421-433 ◽  
Author(s):  
Shunsuke Furukawa ◽  
Chiaki Karaki ◽  
Tomonori Kawano
Keyword(s):  
Fluorescent Protein ◽  
Cellular Migration ◽  
Magnetic Microspheres ◽  
Paramecium Bursaria ◽  
Micro Electro Mechanical Systems ◽  
E Coli ◽  
Symbiotic Algae ◽  
Micro Particles ◽  
Green Fluorescent ◽  
Cellular Movement

It is well known that Paramecium species including green paramecia (Paramecium bursaria) migrate towards the anode when exposed to an electric field in a medium. This type of a cellular movement is known as galvanotaxis. Our previous study revealed that an electric stimulus given to P. bursaria is converted to a galvanotactic cellular movement by involvement of T-type calcium channel on the plasma membrane [Aonuma et al. (2007), Z. Naturforsch. 62 c, 93 - 102]. This phenomenon has attracted the attention of bioengineers in the fields of biorobotics or micro-robotics in order to develop electrically controllable micromachineries. Here, we demonstrate the galvanotactic controls of the cellular migration of P. bursaria in capillary tubes (diameter, 1 - 2 mm; length, 30 - 240 mm). Since the Paramecium cells take up particles of various sizes, we attempted to use the electrically stimulated cells of P. bursaria as the vehicle for transportation of micro-particles in the capillary system. By using apo-symbiotic cells of P. bursaria obtained after forced removal of symbiotic algae, the uptake of the particles could be maximized and visualized. Then, electrically controlled transportations of particle-filled apo-symbiotic P. bursaria cells were manifested. The particles transported by electrically controlled cells (varying in size from nm to μm levels) included re-introduced green algae, fluorescence-labeled polystyrene beads, magnetic microspheres, emerald green fluorescent protein (EmGFP)-labeled cells of E. coli, Indian ink, and crystals of zeolite (hydrated aluminosilicate minerals with a micro-porous structure) and some metal oxides. Since the above demonstrations were successful, we concluded that P. bursaria has a potential to be employed as one of the micro-biorobotic devices used in BioMEMS (biological micro-electro-mechanical systems).

A natural strain of Paramecium bursaria lacking symbiotic algae

2002 ◽  
Vol 38 (1) ◽  
pp. 55-58 ◽  
Author(s):  
Yuki Tonooka ◽  
Tsuyoshi Watanabe
Keyword(s):  
Paramecium Bursaria ◽  
Symbiotic Algae ◽  
Natural Strain

Photosynthetic efficiency of endosymbiotic algae of Paramecium bursaria originating from locations with cold and warm climates

2018 ◽  
Vol 47 (2) ◽  
pp. 202-210
Author(s):  
Katarzyna Możdżeń ◽  
Patrycja Z. Leśnicka ◽  
Tomasz Burnecki ◽  
Sylwia Śliwińska-Wilczewska ◽  
Andrzej Skoczowski ◽  
...  
Keyword(s):  
Photosynthetic Activity ◽  
Cold Climate ◽  
Paramecium Bursaria ◽  
Control Group ◽  
Constant Darkness ◽  
Light Conditions ◽  
Warm Climate ◽  
Symbiotic Algae ◽  
Different Temperatures ◽  
Endosymbiotic Algae

Abstract Paramecium bursaria (Ciliophora) is a cosmopolitan unicellular organism that plays a significant role in aquatic ecosystems. P. bursaria contains symbiotic algae and this association is a mutual symbiosis. The aim of the present study was to determine the activity of photosystem II (PSII) in Chlorella sp. inside P. bursaria cells. Ciliates were incubated for 7 days at different temperatures from 6 to 18°C, under the circadian cycle: 12 h light/12 h dark, at light intensity of 200 μmol m-2 s-1 and under constant darkness conditions. The control group was kept at a temperature of 18°C under constant light conditions. Changes in PSII were monitored using different fluorescence parameters. Differences in responses between endosymbiotic algae of two P. bursaria strains – Ard7 from a warm climate and KD64 from a cold climate – were determined. The highest photosynthetic activity of P. bursaria green endosymbionts was observed at a temperature of 18°C, regardless of the light conditions. Algae from warm climate were more sensitive to cold temperature stress than algae from P. bursaria collected in cold climate.

scholarly journals 2SI-01 Roles of symbiotic algae in green paramecia Paramecium bursaria(2SI Biomimetics focused on the life style of protozoa,The 49th Annual Meeting of the Biophysical Society of Japan)

2011 ◽  
Vol 51 (supplement) ◽  
pp. S21
Author(s):  
Hiroshi Hosoya ◽  
Kazuya Ujihiro ◽  
Eiji Hiraki ◽  
Koyo Tetsukawa ◽  
Yoshihiko Yamashin ◽  
...  
Keyword(s):  
Annual Meeting ◽  
Life Style ◽  
Paramecium Bursaria ◽  
Symbiotic Algae ◽  
Biophysical Society

Symbiotic Algae-free Strains of The Green Paramecium Paramecium bursaria Produced by Herbicide Paraquat

1995 ◽  
Vol 12 (6) ◽  
pp. 807-810 ◽  
Author(s):  
Hiroshi Hosoya ◽  
Kouki Kimura ◽  
Seiji Matsuda ◽  
Miyuki Kitaura ◽  
Tadao Takahashi ◽  
...  
Keyword(s):  
Paramecium Bursaria ◽  
Symbiotic Algae ◽  
Free Strains
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