scholarly journals Molecular identification of fungi colonizing art objects in Thailand and their growth inhibition by local plant extracts

3 Biotech ◽  
2019 ◽  
Vol 9 (10) ◽  
Author(s):  
Witsanu Senbua ◽  
Jesdawan Wichitwechkarn
2021 ◽  
Vol 9 (3) ◽  
pp. 178-189
Author(s):  
Adilson Ricken Schuelter ◽  
Fernando Otaviano Da Silva ◽  
Rosane Dos Santos Grignet ◽  
Debora Pereira ◽  
Rafael Francisco Da Silva ◽  
...  

This work aimed to perform the molecular identification of fungi mango fruits, in addition to verifying the effect of the essential oils for microbial control. Pericarp segments containing injured and healthy areas of mango fruits were initially disinfested, and then incubated in PDA culture medium at 25 °C. From all isolated material, UDC03, UDC04, UDC09 and UDC12 were preselected, which presented homogeneous cultures. The UDC04 and UDC12 isolates were submitted to the sequencing of the ITS region for presenting mycelial growth both greater and morphologically distinct of fungi from Colletotrichum genus. When BLAST is used in species identification, it was observed that UDC04 and UDC12 belong to Botryosphaeria dothidea and Neofusicoccum parvum, respectively. Regarding the control of UDC04, it was observed that BDA culture medium containing 1.5 to 2% of lemongrass and eucalyptus oils resulted in complete inhibition. For UDC12, mycelial growth inhibition was detected with the use of 1% and 1.5% essential oils of eucalyptus and lemongrass, respectively. The chia essential oil was not effective for the isolates control. Finally, the results reveal the importance of molecular identification of these phytopathogens associated with the establishment of an alternative control of fruits due to problems of contamination with chemical products.


2014 ◽  
Vol 8 (34) ◽  
pp. 3171-3177
Author(s):  
Kamel Kossonou Yao ◽  
Clementine Kouakou Amenan ◽  
Daniel Kra Kouam ◽  
H. Tra Bi Fezan ◽  
A. Diallo Hortense ◽  
...  

2010 ◽  
Vol 186 (2) ◽  
pp. 281-285 ◽  
Author(s):  
Kessy Abarenkov ◽  
R. Henrik Nilsson ◽  
Karl-Henrik Larsson ◽  
Ian J. Alexander ◽  
Ursula Eberhardt ◽  
...  

2016 ◽  
Vol 07 (06) ◽  
pp. 949-957 ◽  
Author(s):  
Tavga Sulaiman Rashid ◽  
Kamaruzaman Sijam ◽  
Hayman Kakakhan Awla ◽  
Halimi Mohd Saud ◽  
Jugah Kadir

2021 ◽  
Vol 13 (1) ◽  
pp. 61-74
Author(s):  
N. H. Alzahrani ◽  
K. H. Alamoudi ◽  
A. Kh. Balobaid

Planta Medica ◽  
2019 ◽  
Vol 85 (16) ◽  
pp. 1242-1252
Author(s):  
Yun-Chae Lee ◽  
Sung-Gook Cho ◽  
Sang-Woo Kim ◽  
Jeong Nam Kim

AbstractNumerous chemically synthesized compounds are widely used in oral hygiene products. However, due to their potential risk, there is a need to improve the safety and quality of dental care by seeking alternative control agents such as those naturally found in plant materials. Here we assessed antibacterial potentials of extracts from 100 species of Korean native plants against Streptococcus mutans on cariogenesis. Among those, extracts from five plants (Arctii Fructus, Caryopteris incana, Aralia continentalis, Symplocarpus renifolius, and Lamium amplexicaule) showed a growth inhibition of S. mutans. The five extracts were further individually evaluated for their minimal inhibitory concentration and minimal bactericidal concentration. Interestingly, a synergistic antibacterial activity was observed with the combination of sodium fluoride and the plant extracts. To determine the anti-biofilm activity of plant extracts, S. mutans was treated with increasing concentrations of the extracts in the range from 1250 to 3750 µg/mL. When S. mutans was grown in the defined biofilm medium containing the individual extracts of 47 species, the biofilm amount markedly decreased compared to that of a negative control. Notably, the extract of S. renifolius significantly downregulated the gtf and spaP genes for synthesis of glucan and adhesive proteins in S. mutans, and L. amplexicaule decreased the expression of gtfD gene. Therefore, these results demonstrate that the five plant extracts modulate survival and pathogenesis of S. mutans by growth inhibition and downregulation of the gene(s) implicated in biofilm formation.


2018 ◽  
Vol 46 (2) ◽  
pp. 171-172
Author(s):  
Taketoshi Futatsuya ◽  
Kazushi Anzawa ◽  
Takashi Mochizuki ◽  
Yutaka Hatano

2001 ◽  
Vol 39 (2) ◽  
pp. 138-141 ◽  
Author(s):  
Marcelo V. Silva ◽  
Théo R. Costa ◽  
Márcio R. Costa ◽  
Eduardo C. Ferreira ◽  
Orionalda F. L. Fernandes ◽  
...  

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