Mapping novel QTLs for yield related traits from a popular rice hybrid KRH-2 derived doubled haploid (DH) population

The critical evolutionary step during domestication of major cereals was elimination of seed shattering because the easy-to-shatter trait in wild relatives results in a severe reduction in yield. In this study, we analyzed the QTLs associated with shattering employing a high-density genetic map in doubled haploid (DH) population of rice (Oryza sativaL.). A genetic linkage map was generated with 217 microsatellite markers spanning 2082.4 cM and covering 12 rice chromosomes with an average interval of 9.6 cM between markers based on 120 DHLs derived from a cross between Cheongcheong indica type cultivar and Nagdong japonica type cultivar. In the QTL analysis, five QTLs pertaining to the breaking tensile strength (BTS) were detected in 2013 and 2015. Two regions of the QTLs related to BTS on chromosome 1 and chromosome 6 were detected. Several important genes are distributed in 1 Mbp region of the QTL on chromosome 6 and they are related to the formation of abscission layer. We decide to name this QTLqSh6and the candidate genes in theqSh6region can be employed usefully in further research for cloning.

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Detection of drought tolerance-related QTL in the Plainsman V./Cappelle Desprez doubled haploid wheat population

Cereal Research Communications ◽

10.1007/s42976-021-00229-y ◽

2021 ◽

Author(s):

É. Nagy ◽

Á. Szabó-Hevér ◽

S. Lehoczki-Krsjak ◽

C. Lantos ◽

E. Kiss ◽

...

Keyword(s):

Drought Tolerance ◽

Grain Yield ◽

Doubled Haploid ◽

Agronomic Traits ◽

Abiotic Factors ◽

Phenotypic Variance ◽

Thousand Grain Weight ◽

Dh Population ◽

Wheat Population ◽

Irrigation Levels

AbstractDrought stress is one of the major abiotic factors that significantly reduces wheat grain yield. Improving drought tolerance is a challenge that plant breeders are facing nowadays. In this study, our goal was to identify quantitative trait loci (QTL) in the Plainsman V./Cappelle Desprez doubled haploid (DH) population under drought induced as decreased irrigation (ds) and well-watered (ww) conditions in glasshouse. In total, 54 QTL were detected across the three years in two water regimes linked to 10 drought tolerance-related agronomic traits. Out of the detected QTL regions several have been previously reported. The QTL on chromosome 1A (wPt-744613-wPt-8016) related to thousand grain weight was detected in both ds and ww conditions, explaining the 12.7–17.4% of the phenotypic variance. QTL for grain yield was detected on chromosomes 1A, and 6B in the ds treatment. Numerous QTL was identified under both irrigation levels.

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Mapping QTL for grain yield, yield components, and spike features in a doubled haploid population of bread wheat

Genome ◽

10.1139/g11-017 ◽

2011 ◽

Vol 54 (6) ◽

pp. 517-527 ◽

Cited By ~ 55

Author(s):

Bahram Heidari ◽

Badraldin Ebrahim Sayed-Tabatabaei ◽

Ghodratollah Saeidi ◽

Michael Kearsey ◽

Kazuhiro Suenaga

Keyword(s):

Grain Yield ◽

Doubled Haploid ◽

Yield Components ◽

Triticum Aestivum L ◽

Interval Mapping ◽

Doubled Haploid Population ◽

Dh Population ◽

Spike Number ◽

Haploid Population ◽

Major Qtl

A doubled haploid (DH) population derived from a cross between the Japanese cultivar ‘Fukuho-kumogi’ and the Israeli wheat line ‘Oligoculm’ was used to map genome regions involved in the expression of grain yield, yield components, and spike features in wheat (Triticum aestivum L). A total of 371 markers (RAPD, SSR, RFLP, AFLP, and two morphological traits) were used to construct the linkage map that covered 4190 cM of wheat genome including 28 linkage groups. The results of composite interval mapping for all studied traits showed that some of the quantitative trait loci (QTL) were stable over experiments conducted in 2004 and 2005. The major QTL located in the Hair–Xpsp2999 interval on chromosome 1A controlled the expression of grains/spike (R2 = 12.9% in 2004 and 22.4% in 2005), grain weight/spike (R2 = 21.4% in 2004 and 15.8% in 2005), and spike number (R2 = 15.6% in 2004 and 5.4% in 2005). The QTL for grain yield located on chromosomes 6A, 6B, and 6D totally accounted for 27.2% and 31.7% of total variation in this trait in 2004 and 2005, respectively. Alleles inherited from ‘Oligoculm’ increased the length of spikes and had decreasing effects on spike number. According to the data obtained in 2005, locus Xgwm261 was associated with a highly significant spike length QTL (R2 = 42.33%) and also the major QTL for spikelet compactness (R2 = 26.1%).

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Genetics and molecular mapping of resistance to Plasmodiophora brassicae pathotypes 2, 3, 5, 6, and 8 in rutabaga (Brassica napus var. napobrassica)

Genome ◽

10.1139/gen-2016-0034 ◽

2016 ◽

Vol 59 (10) ◽

pp. 805-815 ◽

Cited By ~ 24

Author(s):

Muhammad Jakir Hasan ◽

Habibur Rahman

Keyword(s):

Brassica Napus ◽

Doubled Haploid ◽

Genetic Basis ◽

Molecular Mapping ◽

Plasmodiophora Brassicae ◽

Single Gene ◽

Genomic Region ◽

Clubroot Disease ◽

Dh Population ◽

Brassica Crops

Clubroot disease, caused by Plasmodiophora brassicae, is a threat to the production of Brassica crops including oilseed B. napus. In Canada, several pathotypes of this pathogen, such as pathotypes 2, 3, 5, 6, and 8, were identified, and resistance to these pathotypes was found in a rutabaga (B. napus var. napobrassica) genotype. In this paper, we report the genetic basis and molecular mapping of this resistance by use of F2, backcross (BC1), and doubled haploid (DH) populations generated from crossing of this rutabaga line to a susceptible spring B. napus canola line. The F1, F2, and BC1 populations were evaluated for resistance to pathotype 3, and the DH population was evaluated for resistance to pathotypes 2, 3, 5, 6, and 8. A 3:1 segregation in F2 and a 1:1 segregation in BC1 were found for resistance to pathotype 3, and a 1:1 segregation was found in the DH population for resistance to all pathotypes. Molecular mapping by using the DH population identified a genomic region on chromosome A8 carrying resistance to all five pathotypes. This suggests that a single gene or a cluster of genes, located in this genomic region, is involved in the control of resistance to these pathotypes.

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Introgression of B-genome chromosomes in a doubled haploid population of Brassica napus × B. carinata

Genome ◽

10.1139/g10-039 ◽

2010 ◽

Vol 53 (8) ◽

pp. 619-629 ◽

Cited By ~ 26

Author(s):

Z. K. Navabi ◽

I. A.P. Parkin ◽

J. C. Pires ◽

Z. Xiong ◽

M. R. Thiagarajah ◽

...

Keyword(s):

Doubled Haploid ◽

Seed Quality ◽

Agronomic Traits ◽

Interspecific Cross ◽

Leptosphaeria Maculans ◽

Erucic Acid Content ◽

Doubled Haploid Population ◽

Dh Population ◽

B Genome ◽

Dh Lines

The Brassica B-genome species possess many valuable agronomic and disease resistance traits. To transfer traits from the B genome of B. carinata into B. napus , an interspecific cross between B. napus and B. carinata was performed and a doubled haploid (DH) population was generated from the BC2S3 generation. Successful production of interspecific DH lines as identified using B-genome microsatellite markers is reported. Five percent of DH lines carry either intact B-genome chromosomes or chromosomes that have deletions. All of the DH lines have linkage group J13/B7 in common. This was further confirmed using B. nigra genomic DNA in a fluorescent in situ hybridization assay where the B-genome chromosomes were visualized and distinguished from the A- and C-genome chromosomes. The 60 DH lines were also evaluated for morphological traits in the field for two seasons and were tested for resistance to blackleg, caused by Leptosphaeria maculans , under greenhouse conditions. Variation in the DH population followed a normal distribution for several agronomic traits and response to blackleg. The lines with B-genome chromosomes were significantly different (p < 0.01) from the lines without B-genome chromosomes for both morphological and seed quality traits such as days to flowering, days to maturity, and erucic acid content.

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Gametophytic selection in a winter × spring barley cross

Genome ◽

10.1139/g91-141 ◽

1991 ◽

Vol 34 (6) ◽

pp. 918-922 ◽

Cited By ~ 9

Author(s):

C. C. Schön ◽

P. M. Hayes ◽

T. K. Blake ◽

S. J. Knapp

Keyword(s):

Segregation Distortion ◽

Doubled Haploid ◽

Doubled Haploids ◽

Spring Barley ◽

Population Based ◽

Doubled Haploid Population ◽

Dh Population ◽

Gametophytic Selection ◽

Segregation Ratios ◽

Haploid Population

Segregation distortion and the consequences of gametophytic selection were assessed in a winter × spring barley cross by comparing segregation of enzyme, storage protein, DNA, and morphological markers in three populations derived from the same cross: a control F2 (F2C), a doubled-haploid (DH) population, and an F2 derived from F1 plants self-pollinated at 10 °C (F2T). Segregation distortion was present in the F2T and the doubled-haploid population. Based on a comparison of the F2C and the F2T, gametophytic selection as a consequence of self-pollination at 10 °C was operative on chromosome 7 in regions linked to Rrn2. Segregation distortion in favor of the winter parent was found in the doubled-haploid population. There were significant deviations from expected segregation ratios at two loci, but only at one of the loci was the gene number significantly different from the F2C. Despite segregation distortion, the doubled-haploid population should be suitable for linkage analyses, as estimates of recombination based on F2 and doubled-haploid data were in close agreement.Key words: Hordeum vulgare, segregation distortion, doubled haploids, gametophytic selection, cold tolerance.

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QTL Analysis of Plant Height based on Doubled Haploid (DH) Population derived from PTSMS Wheat

Molecular Plant Breeding ◽

10.5376/mpb.2011.02.0013 ◽

2011 ◽

Cited By ~ 1

Author(s):

Liping Zhang ◽

Xiaoqin Xu ◽

Chanping Zhao ◽

Fuhua Shan ◽

Shaohua Yuan ◽

...

Keyword(s):

Plant Height ◽

Doubled Haploid ◽

Qtl Analysis ◽

Dh Population

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Molecular Mapping of QTL for Fusarium Head Blight Resistance in a Doubled Haploid Population of Chinese Bread Wheat

Plant Disease ◽

10.1094/pdis-06-20-1186-re ◽

2020 ◽

Author(s):

Zhanwang Zhu ◽

Xiaoting Xu ◽

Luping Fu ◽

Fengju Wang ◽

Yachao Dong ◽

...

Keyword(s):

Fusarium Head Blight ◽

Plant Height ◽

Doubled Haploid ◽

Agronomic Traits ◽

Natural Infection ◽

Snp Array ◽

Head Blight ◽

Dh Population ◽

Anther Extrusion ◽

Fhb Resistance

Fusarium head blight (FHB) is a destructive disease of wheat worldwide, particularly in China. To map genetic loci underlying FHB resistance, a doubled haploid (DH) population consisting of 174 lines was developed from a cross between widely grown Chinese cultivars Yangmai 16 and Zhongmai 895. The DH population and parents were evaluated in field nurseries at Wuhan in 2016–2017 and 2017–2018 with both spray inoculation and natural infection, and at Jingzhou in 2017–2018 with grain-spawn inoculation. The DH lines were genotyped with a wheat 660K SNP array. FHB index, plant height, anther extrusion, and days to anthesis were recorded and used for QTL analysis. Seven QTL for FHB resistance were mapped to chromosome arms 3BL, 4AS, 4BS, 4DS, 5AL, 6AL, and 6BS, in at least two environments. QFhb.caas-4BS and QFhb.caas-4DS co-located with semi-dwarfing alleles Rht-B1b and Rht-D1b, respectively, and were also associated with anther extrusion. The other five QTL were genetically independent of the agronomic traits, indicating their potential value in breeding for FHB resistance. Based on correlations between FHB indices and agronomic traits in this population, we concluded that increasing plant height to some extent would enhance FHB resistance, anther extrusion played a more important role in environments with less severe FHB, and days to anthesis was independent of FHB response when viewed across years. PCR-based markers were developed for the 3BL and 5AL QTL, which were detected in more than three environments. The InDel marker InDel_AX-89588684 for QFhb.caas-5AL was also validated on a wheat panel, confirming its effectiveness for marker-assisted breeding for improvement of FHB resistance.

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Assessment of heterotic potential and combining ability of immortal restorer lines derived from an elite rice hybrid, KRH-2, for the development of superior rice hybrids

10.1101/2020.11.09.373985 ◽

2020 ◽

Author(s):

Swapnil Ravindra Kulkarni ◽

Balachandran SM ◽

Fiyaz RA ◽

Sruthi K ◽

Divya Balakrishnan ◽

...

Keyword(s):

Grain Yield ◽

Doubled Haploid ◽

Combining Ability ◽

Recombinant Inbred Lines ◽

Flag Leaf ◽

Yield Component ◽

Leaf Length ◽

Spikelet Fertility ◽

Flowering Plant ◽

Rice Hybrid

AbstractPresent investigation was carried out to assess the heterotic potential and combining ability of immortal restorer lines [consisting of two recombinant inbred lines (RILs) and two doubled haploid lines (DHLs)] developed from an elite rice hybrid, KRH-2 by crossing them with three popular WA-CMS lines, IR58025A, CRMS32A and APMS6A through line × tester analysis. The doubled haploid line 1 (DHL-1) was observed to be a good general combiner for total grain yield per plant (YLD) and other yield component traits and among the CMS lines, IR58025A was observed to be the best combiner as it showed positive significant values for the traits viz., total grain yield per plant, panicle length and spikelet fertility. Higher preponderance of the variance associated with specific combining ability (SCA) as compared to general combining ability (GCA) variance was observed for most of the traits indicated the predominant role of non-additive gene action in the expression of the traits. Out of twelve novel crosses between the immortal restorer lines derived from KRH-2 and the WA-CMS lines, 66.66% (eight crosses) showed significant and desirable SCA effects for the traits viz., total grain yield per plant, days to fifty percent flowering, plant height, flag leaf length, flag leaf width, number of filled grains per panicle and spikelet fertility. Two crosses IR58025A/RIL-24 and CRMS32A/RIL-24 were observed to be the most promising cross combinations showing standard heterosis of >50% for YLD trait (as compared with KRH-2) with higher prevalence of GCA and SCA, respectively. Heterotic yield advantage of IR58025A/RIL-24 and CRMS32A/RIL-24 was 77.05% and 54.74%, respectively over KRH-2 and these can be utilized for developing commercial hybrids. The present study also indicates the potentiality of RILs in providing useful parental lines for developing heterotic hybrids which are hard to get from outside sources in the new intellectual property regime.

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