Tri-layered silk fibroin and poly-ɛ-caprolactone small diameter vascular grafts tested in vitro and in vivo

2015 ◽  
Vol 23 (10) ◽  
pp. 924-936 ◽  
Author(s):  
A. Reum Park ◽  
Young-Hwan Park ◽  
Hyun Jeong Kim ◽  
Min-Keun Kim ◽  
Seong-Gon Kim ◽  
...  
Keyword(s):  
Silk Fibroin ◽  
Vascular Grafts ◽  
Small Diameter ◽  
Small Diameter Vascular Grafts

scholarly journals Controllable synthesis of biomimetic nano/submicro-fibrous tubes for potential small-diameter vascular grafts

2020 ◽  
Vol 8 (26) ◽  
pp. 5694-5706
Author(s):  
Yizao Wan ◽  
Shanshan Yang ◽  
Mengxia Peng ◽  
Miguel Gama ◽  
Zhiwei Yang ◽  
...  
Keyword(s):  
Cellulose Acetate ◽  
Bacterial Cellulose ◽  
Vascular Grafts ◽  
Small Diameter ◽  
Controllable Synthesis ◽  
Small Diameter Vascular Grafts

A novel small-diameter graft consisting of nanofibrous bacterial cellulose and submicrofibrous cellulose acetate was prepared and evaluated in vitro and in vivo.

Mechanical and degradation properties of small-diameter vascular grafts in an in vitro biomimetic environment

2019 ◽  
Vol 33 (8) ◽  
pp. 1017-1034 ◽  
Author(s):  
Xiangshun Li ◽  
Huijing Zhao
Keyword(s):  
Lactic Acid ◽  
Tensile Properties ◽  
Silk Fibroin ◽  
Vascular Grafts ◽  
Small Diameter ◽  
Degradation Behavior ◽  
Degradation Properties ◽  
Small Diameter Vascular Grafts ◽  
Retention Strength

Small-diameter vascular grafts may fail after implantation due to various reasons from mechanical and biological aspects. In order to evaluate the mechanical durability of small-diameter vascular grafts after implantation, an artificial vascular biomimetic environment that can simulate body temperature, the liquid environment outside the vessel, and continuous blood flow and pulsatile pressure was constructed. This device can be used as a “pre-test” prior to animal experiments to explore the changes of mechanical and degradation properties in the long-term in vivo environment. At the same time, braided tube-reinforced silk fibroin/poly (l-lactic acid-co-ε-caprolactone) small-diameter vascular grafts were fabricated and tested under the biomimetic environment. Mechanical changes, including tensile properties, suture retention strength, compliance, and degradation behavior of the braided tube-reinforced poly (l-lactic acid-co-ε-caprolactone)/silk fibroin small-diameter vascular grafts were explored over various periods of time in the biomimetic environment. The results shown that under a period of testing in the in vitro biomimetic environment, the comprehensive mechanical properties (including tensile properties, suture retention strength, estimated-bursting pressure, and compliance) of small-diameter vascular grafts exhibited varying degrees of changes but that there was no obvious degradation behavior in the short term.

Small diameter electrospun silk fibroin vascular grafts: Mechanical properties, in vitro biodegradability, and in vivo biocompatibility

2015 ◽  
Vol 54 ◽  
pp. 101-111 ◽  
Author(s):  
Valentina Catto ◽  
Silvia Farè ◽  
Irene Cattaneo ◽  
Marina Figliuzzi ◽  
Antonio Alessandrino ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Silk Fibroin ◽  
Vascular Grafts ◽  
Small Diameter

scholarly journals Comparative Evaluation on Impacts of Fibronectin, Heparin–Chitosan, and Albumin Coating of Bacterial Nanocellulose Small-Diameter Vascular Grafts on Endothelialization In Vitro

Nanomaterials ◽  
2021 ◽  
Vol 11 (8) ◽  
pp. 1952
Author(s):  
Max Wacker ◽  
Jan Riedel ◽  
Heike Walles ◽  
Maximilian Scherner ◽  
George Awad ◽  
...  
Keyword(s):  
Low Density Lipoprotein ◽  
Vascular Grafts ◽  
Small Diameter ◽  
Density Lipoprotein ◽  
Human Albumin ◽  
Water Soluble ◽  
Bacterial Nanocellulose ◽  
Functionally Impaired ◽  
Small Diameter Vascular Grafts

In this study, we contrast the impacts of surface coating bacterial nanocellulose small-diameter vascular grafts (BNC-SDVGs) with human albumin, fibronectin, or heparin–chitosan upon endothelialization with human saphenous vein endothelial cells (VEC) or endothelial progenitor cells (EPC) in vitro. In one scenario, coated grafts were cut into 2D circular patches for static colonization of a defined inner surface area; in another scenario, they were mounted on a customized bioreactor and subsequently perfused for cell seeding. We evaluated the colonization by emerging metabolic activity and the preservation of endothelial functionality by water soluble tetrazolium salts (WST-1), acetylated low-density lipoprotein (AcLDL) uptake assays, and immune fluorescence staining. Uncoated BNC scaffolds served as controls. The fibronectin coating significantly promoted adhesion and growth of VECs and EPCs, while albumin only promoted adhesion of VECs, but here, the cells were functionally impaired as indicated by missing AcLDL uptake. The heparin–chitosan coating led to significantly improved adhesion of EPCs, but not VECs. In summary, both fibronectin and heparin–chitosan coatings could beneficially impact the endothelialization of BNC-SDVGs and might therefore represent promising approaches to help improve the longevity and reduce the thrombogenicity of BNC-SDVGs in the future.

scholarly journals Review: Tissue Engineering of Small-Diameter Vascular Grafts and Their In Vivo Evaluation in Large Animals and Humans

Cells ◽  
2021 ◽  
Vol 10 (3) ◽  
pp. 713
Author(s):  
Shu Fang ◽  
Ditte Gry Ellman ◽  
Ditte Caroline Andersen
Keyword(s):  
Animal Models ◽  
Vascular Grafts ◽  
Small Diameter ◽  
Large Animal ◽  
Large Animal Models ◽  
Graft Outcome ◽  
Limited Success ◽  
Large Animals

To date, a wide range of materials, from synthetic to natural or a mixture of these, has been explored, modified, and examined as small-diameter tissue-engineered vascular grafts (SD-TEVGs) for tissue regeneration either in vitro or in vivo. However, very limited success has been achieved due to mechanical failure, thrombogenicity or intimal hyperplasia, and improvements of the SD-TEVG design are thus required. Here, in vivo studies investigating novel and relative long (10 times of the inner diameter) SD-TEVGs in large animal models and humans are identified and discussed, with emphasis on graft outcome based on model- and graft-related conditions. Only a few types of synthetic polymer-based SD-TEVGs have been evaluated in large-animal models and reflect limited success. However, some polymers, such as polycaprolactone (PCL), show favorable biocompatibility and potential to be further modified and improved in the form of hybrid grafts. Natural polymer- and cell-secreted extracellular matrix (ECM)-based SD-TEVGs tested in large animals still fail due to a weak strength or thrombogenicity. Similarly, native ECM-based SD-TEVGs and in-vitro-developed hybrid SD-TEVGs that contain xenogeneic molecules or matrix seem related to a harmful graft outcome. In contrast, allogeneic native ECM-based SD-TEVGs, in-vitro-developed hybrid SD-TEVGs with allogeneic banked human cells or isolated autologous stem cells, and in-body tissue architecture (IBTA)-based SD-TEVGs seem to be promising for the future, since they are suitable in dimension, mechanical strength, biocompatibility, and availability.

scholarly journals In vitro hemo- and cytocompatibility of bacterial nanocelluose small diameter vascular grafts: Impact of fabrication and surface characteristics.

PLoS ONE ◽  
2020 ◽  
Vol 15 (6) ◽  
pp. e0235168 ◽  
Author(s):  
Max Wacker ◽  
Viktoria Kießwetter ◽  
Ingo Slottosch ◽  
George Awad ◽  
Adnana Paunel-Görgülü ◽  
...  
Keyword(s):  
Vascular Grafts ◽  
Small Diameter ◽  
Surface Characteristics ◽  
Small Diameter Vascular Grafts

Small-diameter vascular grafts of Bombyx mori silk fibroin prepared by a combination of electrospinning and sponge coating

2010 ◽  
Vol 64 (16) ◽  
pp. 1786-1788 ◽  
Author(s):  
Michiko Sato ◽  
Yasumoto Nakazawa ◽  
Rui Takahashi ◽  
Kimie Tanaka ◽  
Masataka Sata ◽  
...  
Keyword(s):  
Bombyx Mori ◽  
Silk Fibroin ◽  
Vascular Grafts ◽  
Small Diameter ◽  
Bombyx Mori Silk ◽  
Small Diameter Vascular Grafts

Development of Small-Diameter Vascular Grafts Based on Silk Fibroin Fibers from Bombyx mori for Vascular Regeneration

2011 ◽  
Vol 22 (1-3) ◽  
pp. 195-206 ◽  
Author(s):  
Yasumoto Nakazawa ◽  
Michiko Sato ◽  
Rui Takahashi ◽  
Derya Aytemiz ◽  
Chiyuki Takabayashi ◽  
...  
Keyword(s):  
Bombyx Mori ◽  
Silk Fibroin ◽  
Vascular Grafts ◽  
Small Diameter ◽  
Vascular Regeneration ◽  
Small Diameter Vascular Grafts

scholarly journals In vitro and in vivo Evaluation of Hemocompatibility of Silk Fibroin Based Artificial Vascular Grafts

2014 ◽  
Vol 6 (2) ◽  
Author(s):  
Derya Aytemiz ◽  
Yu Suzuki ◽  
Tomoko Shindo ◽  
Toshiki Saotome ◽  
Ryou Tanaka ◽  
...  
Keyword(s):  
Silk Fibroin ◽  
Vascular Grafts ◽  
In Vivo Evaluation
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