Cuminum cyminum Linn. and Coriandrum sativum Linn. extracts modulate Chromium genotoxicity in Allium cepa chromosomal aberration assay

The Nucleus ◽  
2011 ◽  
Vol 54 (2) ◽  
pp. 99-105 ◽  
Author(s):  
Arti Sharma ◽  
Manish Kumar ◽  
Satwinderjeet Kaur
Keyword(s):  
Allium Cepa ◽  
Chromosomal Aberration ◽  
Coriandrum Sativum ◽  
Cuminum Cyminum ◽  
Chromosomal Aberration Assay

Allium cepa Root Chromosomal Aberration Assay: An Application in Assessing Anti-genotoxic Potential of Ashwagandha

2017 ◽  
pp. 69-79
Author(s):  
Rajneet Kour Soodan ◽  
Ashita Sharma ◽  
Mandeep Kaur ◽  
Jatinder Kaur Katnoria ◽  
Avinash Kaur Nagpal
Keyword(s):  
Allium Cepa ◽  
Chromosomal Aberration ◽  
Genotoxic Potential ◽  
Chromosomal Aberration Assay

scholarly journals The Effect of Induced Acid Rain; Allium cepa Chromosomal Aberration Assay

2021 ◽  
Vol 9 (3) ◽  
pp. 89-97
Author(s):  
  Renjith Raju ◽  
Allwin George Paul ◽  
Unnis P. Aguilor ◽  
Julius T. Capili
Keyword(s):  
Acid Rain ◽  
Allium Cepa ◽  
Chromosomal Aberration ◽  
Chromosomal Aberration Assay

scholarly journals Allium Cepa Root Chromosomal Aberration Assay: A Review

2013 ◽  
Vol 1 (03) ◽  
pp. 105-119 ◽  
Author(s):  
Namita Khanna ◽  
Sonia Sharma
Keyword(s):  
Allium Cepa ◽  
Chromosomal Aberration ◽  
Chromosomal Aberrations ◽  
Mitotic Cycle ◽  
Chromosomal Abnormalities ◽  
Higher Plants ◽  
Test Organism ◽  
Short Term ◽  
Chromosomal Aberration Assay ◽  
Antigenotoxic Effects

Higher plants, an important material for genetic tests to monitor various pollutant present in the environment. Among the plant species, Alium cepa has been used to evaluate chromosome aberrations and disturbances in the mitotic cycle. Now days, it has been used to assess a great number of genotoxic/antigenotoxic agents, which contributes to its increasing application in environmental monitoring. The A. cepa is commonly used as a test organism because it is cheap, easily available and handled and has advantages over other short-term tests. Among the endpoints of A. cepa root chromosomal aberrations, detection of chromosomal aberration have been the most used one to detect genotoxicity/ antigenotoxicity along the years. The mitotic index and chromosomal abnormalities are used to evaluate genotoxicity and micronucleus analysis used to verify mutagenicity of different chemicals. The Allium cepa root chromosomal aberration assay is widely used to determine genotoxic and antigenotoxic effects of different plant extracts.

Isolation of 24-epibrassinolide from leaves of Aegle marmelos and evaluation of its antigenotoxicity employing Allium cepa chromosomal aberration assay

2007 ◽  
Vol 54 (3) ◽  
pp. 217-224 ◽  
Author(s):  
Nishi Sondhi ◽  
Renu Bhardwaj ◽  
Satwinderjeet Kaur ◽  
Neeraj Kumar ◽  
Bikram Singh
Keyword(s):  
Allium Cepa ◽  
Chromosomal Aberration ◽  
Aegle Marmelos ◽  
Chromosomal Aberration Assay

Utilization of rat lymphocytes for the in vitro chromosomal aberration assay

Mutagenesis ◽  
1989 ◽  
Vol 4 (2) ◽  
pp. 147-153 ◽  
Author(s):  
Anil K. Sinha ◽  
B.Bhaskar Gollapudi ◽  
V.Ann Linscombe ◽  
Mary L. McClintock
Keyword(s):  
Chromosomal Aberration ◽  
Rat Lymphocytes ◽  
Chromosomal Aberration Assay

scholarly journals Effect of Ferrous Gluconate on Chromosomal Abnormality Index of Allium Cepa Root Tip

2021 ◽  
Vol 9 (4) ◽  
pp. 755-758
Author(s):  
Nergis Kaya
Keyword(s):  
Allium Cepa ◽  
Chromosomal Aberration ◽  
Chromosomal Abnormality ◽  
Genotoxic Effect ◽  
Root Tip ◽  
Control Group ◽  
Root Tips ◽  
Ferrous Gluconate ◽  
Allium Cepa L ◽  
Abnormality Index

In completed research, ferrous gluconate -a food additive- used to preserve black color to prevent discoloration during storage in ripe black olives, and Allium cepa L. species. A. cepa L. roots were treated with different doses of ferrous gluconate. The effective concentration EC50 (0.068 g/l) was determined. A. cepa root tips were treated with EC50/2 (0.034 g/l), EC50 (0.068 g/l), 2XEC50 (0.136 g/l) dose for 24, 48, 72 hours, and afterward, the root tips were prepared for observation under the light microscope according to the method of preparing mitotic preparation. Chromosomal abnormality index (CAI) and genotoxic effect of ferrous gluconate in A. cepa root tip cells were determined. Repeated measurement ANOVA and TUKEY multiple comparison tests were used to investigate the effect of time and dose together on genotoxicity. C-mitosis, polyploidy, polar shifting in anaphase, polar shifting in telophase, equatorial plate shifting, laggard chromosome was observed by microscope. The highest CAI (70.16±4.85) was observed at 72h for 2XEC50 dose. Chromosomal aberration is also observed in control group. While the most common chromosomal aberration is determined as C-mitosis; The least observed chromosomal aberration is determined as polyploidy. Research results revealed that ferrous gluconate has a genotoxic effect on the root tip of A. cepa.

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