Allium cepa Root Chromosomal Aberration Assay: An Application in Assessing Anti-genotoxic Potential of Ashwagandha

2017 ◽  
pp. 69-79
Author(s):  
Rajneet Kour Soodan ◽  
Ashita Sharma ◽  
Mandeep Kaur ◽  
Jatinder Kaur Katnoria ◽  
Avinash Kaur Nagpal
Keyword(s):  
Allium Cepa ◽  
Chromosomal Aberration ◽  
Genotoxic Potential ◽  
Chromosomal Aberration Assay

scholarly journals The Effect of Induced Acid Rain; Allium cepa Chromosomal Aberration Assay

2021 ◽  
Vol 9 (3) ◽  
pp. 89-97
Author(s):  
  Renjith Raju ◽  
Allwin George Paul ◽  
Unnis P. Aguilor ◽  
Julius T. Capili
Keyword(s):  
Acid Rain ◽  
Allium Cepa ◽  
Chromosomal Aberration ◽  
Chromosomal Aberration Assay

scholarly journals Allium Cepa Root Chromosomal Aberration Assay: A Review

2013 ◽  
Vol 1 (03) ◽  
pp. 105-119 ◽  
Author(s):  
Namita Khanna ◽  
Sonia Sharma
Keyword(s):  
Allium Cepa ◽  
Chromosomal Aberration ◽  
Chromosomal Aberrations ◽  
Mitotic Cycle ◽  
Chromosomal Abnormalities ◽  
Higher Plants ◽  
Test Organism ◽  
Short Term ◽  
Chromosomal Aberration Assay ◽  
Antigenotoxic Effects

Higher plants, an important material for genetic tests to monitor various pollutant present in the environment. Among the plant species, Alium cepa has been used to evaluate chromosome aberrations and disturbances in the mitotic cycle. Now days, it has been used to assess a great number of genotoxic/antigenotoxic agents, which contributes to its increasing application in environmental monitoring. The A. cepa is commonly used as a test organism because it is cheap, easily available and handled and has advantages over other short-term tests. Among the endpoints of A. cepa root chromosomal aberrations, detection of chromosomal aberration have been the most used one to detect genotoxicity/ antigenotoxicity along the years. The mitotic index and chromosomal abnormalities are used to evaluate genotoxicity and micronucleus analysis used to verify mutagenicity of different chemicals. The Allium cepa root chromosomal aberration assay is widely used to determine genotoxic and antigenotoxic effects of different plant extracts.

Isolation of 24-epibrassinolide from leaves of Aegle marmelos and evaluation of its antigenotoxicity employing Allium cepa chromosomal aberration assay

2007 ◽  
Vol 54 (3) ◽  
pp. 217-224 ◽  
Author(s):  
Nishi Sondhi ◽  
Renu Bhardwaj ◽  
Satwinderjeet Kaur ◽  
Neeraj Kumar ◽  
Bikram Singh
Keyword(s):  
Allium Cepa ◽  
Chromosomal Aberration ◽  
Aegle Marmelos ◽  
Chromosomal Aberration Assay

Evaluation of genotoxicity of pan masala employing chromosomal aberration and micronucleus assay in bone marrow cells of the mice

2009 ◽  
Vol 25 (7) ◽  
pp. 467-471 ◽  
Author(s):  
BN Mojidra ◽  
K. Archana ◽  
AK Gautam ◽  
Y. Verma ◽  
BC Lakkad ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Chromosome Aberration ◽  
Chromosomal Aberration ◽  
Bone Marrow Cells ◽  
Micronucleus Assay ◽  
Control Group ◽  
Genotoxic Potential ◽  
Polychromatic Erythrocytes ◽  
Significant Difference ◽  
Marrow Cells

Pan masala is commonly consumed in south-east Asian and other oriental countries as an alternate of tobacco chewing and smoking. Genotoxic potential of pan masala (pan masala plain and pan masala with tobacco known as gutkha) was evaluated employing chromosome aberration (CA) and micronucleus (MN) assay in vivo. Animals were exposed to three different doses (0.5%, 1.5% and 3%) of pan masala plain (PMP) and gutkha (PMT) through feed for a period of 6 months and micronucleus and chromosomal aberrations were studied in the bone marrow cells. Induction of mean micronuclei in polychromatic erythrocytes (MNPCE) and normochromatic erythrocyte (MNNCE) was higher in both types of pan masala treated groups with respect to control group. Both pan masala plain and gutkha treatment significantly induced the frequency of MNPCE and MNNCE in the bone marrow cells, indicating the genotoxic potential. Furthermore, slight decline in the ratio of polychromatic erythrocytes to normochromatic erythrocytes was also noticed, suggesting the cytotoxic potential even though the ratio was statistically non significant. A dose-dependent, significant increase in chromosome aberration was observed in both types of pan masala treated mice with respect to control. However, no significant difference in micronucleus and chromosomal aberration induction was noticed between two types of pan masala exposed (PMP and PMT) groups. Results suggest that both types of pan masala, i.e. plain and gutkha, have genotoxic potential.

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