scholarly journals Epigenetic reprogramming, gene expression and in vitro development of porcine SCNT embryos are significantly improved by a histone deacetylase inhibitor—m-carboxycinnamic acid bishydroxamide (CBHA)

2014 ◽  
Vol 5 (5) ◽  
pp. 382-393 ◽  
Author(s):  
Yuran Song ◽  
Tang Hai ◽  
Ying Wang ◽  
Runfa Guo ◽  
Wei Li ◽  
...  
Keyword(s):  
Gene Expression ◽  
Histone Deacetylase ◽  
Histone Deacetylase Inhibitor ◽  
Epigenetic Reprogramming ◽  
In Vitro Development ◽  
Deacetylase Inhibitor ◽  
Vitro Development

57 GENE EXPRESSION PATTERN OF MINIATURE PIG SOMATIC CELL NUCLEAR TRANSFER EMBRYOS TREATED WITH THE HISTONE DEACETYLASE INHIBITOR SCRIPTAID

2011 ◽  
Vol 23 (1) ◽  
pp. 134
Author(s):  
C. H. Park ◽  
S. G. Lee ◽  
H. J. Lee ◽  
T. K. Jung ◽  
Y. H. Jeong ◽  
...  
Keyword(s):  
Gene Expression ◽  
Histone Deacetylase ◽  
Expression Pattern ◽  
Histone Deacetylase Inhibitor ◽  
Gene Expression Pattern ◽  
Control Group ◽  
Developmental Potential ◽  
Deacetylase Inhibitor

It was recently shown that treatment of cloned embryos with histone deacetylase inhibitors improves efficiency for the success rate of developmental potential to term in several species. The objective of the present study was to investigate the influence of the histone deacetylase inhibitor Scriptaid (Sc) on in vitro development in early porcine SCNT embryos and on their gene expression pattern. Based on the findings of previous porcine studies (Zhao et al. 2009), the reconstructed oocytes were treated with 500 nM Scriptaid for 14 to 16 h after post-fusion activation (6-DMAP/demecolcine). In our preliminary study, blastocyst rate significantly increased in the Sc-treated group, compared with the control group (25.1 ± 2.8% and 13.8 ± 1.9%, respectively, P < 0.05). We determined gene expression using quantitative real-time RT-PCR. The results showed that OCT3/4 gene was expressed at a similar level in in vivo and SCNT blastocysts with/without Sc. IGF2 and H19 genes tended to be highly expressed in both SCNT blastocysts with (1.6-fold and 3.1-fold, respectively) and without (2.0-fold and 5.8-fold, respectively) Sc than that of the in vivo blastocysts. We found differences in imprinted gene expression patterns between in vivo and cloned blastocysts. Expression of H19 and IGF2 in SCNT blastocysts after Scriptaid treatment decreased towards the expression levels of in vivo blastocysts. These results indicated that Scriptaid treatment in SCNT embryos may also have beneficial effects on in vitro developmental competence as well as their gene expression pattern.

scholarly journals Transcriptomic analysis reveals niche gene expression effects of beta-hydroxybutyrate in primary myotubes

2021 ◽  
Author(s):  
Philip M. M. Ruppert ◽  
Guido J. E. J. Hooiveld ◽  
Roland W. J. Hangelbroek ◽  
Anja Zeigerer ◽  
Sander Kersten
Keyword(s):  
Gene Expression ◽  
Histone Deacetylase ◽  
Histone Deacetylase Inhibitor ◽  
Tca Cycle ◽  
Cell Types ◽  
C2c12 Myotubes ◽  
Signaling Molecule ◽  
Beneficial Effects ◽  
Deacetylase Inhibitor

ABSTRACTVarious forms of fasting, including time-restricted feeding, alternate day fasting, and periodic fasting have shown promise in clinical and pre-clinical studies to normalize body weight, improve metabolic health, and protect against disease. Recent studies suggest that β-hydroxybutyrate (βOHB), a characteristic ketone body of the fasted metabolic state, acts as a potential signaling molecule mediating the beneficial effects of the various forms of fasting, potentially by acting as a histone deacetylase inhibitor. In the first part we investigated whether βOHB, in comparison to the well-established histone deacetylase inhibitor butyrate, influences cellular differentiation in vitro. In C2C12 myotubes, 3T3-L1 adipocytes, and THP-1 monocytes, millimolar concentrations of βOHB did not alter differentiation, as determined by gene expression and histological assessment, whereas equimolar concentrations of butyrate potently impaired differentiation in all cell types. RNA-sequencing revealed that unlike butyrate, βOHB minimally impacted gene expression in adipocytes, macrophages, and hepatocytes. However, in myocytes, βOHB upregulated genes involved in the TCA cycle and oxidative phosphorylation, while downregulating genes belonging to cytokine and chemokine signal transduction. Overall, our data do not support the notion that βOHB serves as a powerful signaling molecule regulating gene expression in adipocytes, macrophages and hepatocytes, but suggest that βOHB may act as a niche signaling molecule in muscle.

CRA-026440: a potent, broad-spectrum, hydroxamic histone deacetylase inhibitor with antiproliferative and antiangiogenic activity in vitro and in vivo

2006 ◽  
Vol 5 (7) ◽  
pp. 1693-1701 ◽  
Author(s):  
Z. Alexander Cao ◽  
Kathryn E. Bass ◽  
Sriram Balasubramanian ◽  
Liang Liu ◽  
Brian Schultz ◽  
...  
Keyword(s):  
Histone Deacetylase ◽  
Broad Spectrum ◽  
Histone Deacetylase Inhibitor ◽  
Antiangiogenic Activity ◽  
Deacetylase Inhibitor

Dual targeting of mTORC1/C2 complexes enhances histone deacetylase inhibitor-mediated anti-tumor efficacy in primary HCC cancer in vitro and in vivo

2012 ◽  
Vol 56 (1) ◽  
pp. 176-183 ◽  
Author(s):  
Huanjie Shao ◽  
Chun Gao ◽  
Haikuo Tang ◽  
Hao Zhang ◽  
Lewis R. Roberts ◽  
...  
Keyword(s):  
Histone Deacetylase ◽  
Histone Deacetylase Inhibitor ◽  
Dual Targeting ◽  
Deacetylase Inhibitor

Effects of FK228, a novel histone deacetylase inhibitor, on human lymphoma U-937 cells in vitro and in vivo

2002 ◽  
Vol 64 (7) ◽  
pp. 1079-1090 ◽  
Author(s):  
Yuka Sasakawa ◽  
Yoshinori Naoe ◽  
Takeshi Inoue ◽  
Tatsuya Sasakawa ◽  
Masahiko Matsuo ◽  
...  
Keyword(s):  
Histone Deacetylase ◽  
Histone Deacetylase Inhibitor ◽  
Deacetylase Inhibitor ◽  
Human Lymphoma

Effects of the HDAC inhibitor scriptaid on the in vitro development of bovine embryos and on imprinting gene expression levels

2018 ◽  
Vol 110 ◽  
pp. 79-85 ◽  
Author(s):  
R. Laguna-Barraza ◽  
M.J. Sánchez-Calabuig ◽  
A. Gutiérrez-Adán ◽  
D. Rizos ◽  
S. Pérez-Cerezales
Keyword(s):  
Gene Expression ◽  
Hdac Inhibitor ◽  
Bovine Embryos ◽  
In Vitro Development ◽  
Expression Levels ◽  
Imprinting Gene ◽  
Vitro Development ◽  
Gene Expression Levels
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