Virus indexing for Sugarcane Yellow Leaf Virus (SCYLV) in field varieties and in vitro regenerated plantlets of sugarcane

2017 ◽  
Vol 46 (5) ◽  
pp. 433-439 ◽  
Author(s):  
Suresh Madugula ◽  
Uma Devi Gali
Keyword(s):  
Yellow Leaf ◽  
Sugarcane Yellow Leaf Virus ◽  
Virus Indexing ◽  
Regenerated Plantlets ◽  
Leaf Virus

scholarly journals Detection of Sugarcane yellow leaf virus in Quarantine and Production of Virus-free Sugarcane by Apical Meristem Culture

Plant Disease ◽  
2001 ◽  
Vol 85 (11) ◽  
pp. 1177-1180 ◽  
Author(s):  
M. Chatenet ◽  
C. Delage ◽  
M. Ripolles ◽  
M. Irey ◽  
B. E. L. Lockhart ◽  
...  
Keyword(s):  
Apical Meristem ◽  
False Negative ◽  
Hot Water ◽  
Meristem Culture ◽  
Vascular Bundles ◽  
Yellow Leaf ◽  
Rt Pcr ◽  
Sugarcane Yellow Leaf Virus ◽  
Leaf Virus

Sugarcane yellow leaf virus (SCYLV) was detected for the first time in 1996 in the Centre de Coopération Internationale en Recherche Agronomique pour le Développement (CIRAD) sugarcane quarantine at Montpellier by reverse transcription-polymerase chain reaction (RT-PCR) in varieties from Brazil, Florida, Mauritius, and Réunion. Between 1997 and 2000, the virus was found by RT-PCR and/or tissue-blot immunoassay (TBIA) in additional varieties from Barbados, Cuba, Guadeloupe, Indonesia, Malaysia, Philippines, Puerto Rico, and Taiwan, suggesting a worldwide distribution of the pathogen. An excellent correlation was observed between results obtained for the two diagnostic techniques. However, even though only a few false negative results were obtained by either technique, both are now used to detect SCYLV in CIRAD's sugarcane quarantine in Montpellier. The pathogen was detected by TBIA or RT-PCR in all leaves of sugarcane foliage, but the highest percentage of infected vascular bundles was found in the top leaves. The long hot water treatment (soaking of cuttings in water at 25°C for 2 days and then at 50°C for 3 h) was ineffective in eliminating SCYLV from infected plants. Sugarcane varieties from various origins were grown in vitro by apical bud culture and apical meristem culture, and the latter proved to be the most effective method for producing SCYLV-free plants.

scholarly journals Variation in Infection Capacity and in Virulence Exists Between Genotypes of Sugarcane yellow leaf virus

Plant Disease ◽  
2007 ◽  
Vol 91 (3) ◽  
pp. 253-259 ◽  
Author(s):  
Youssef Abu Ahmad ◽  
Laurent Costet ◽  
Jean-Heinrich Daugrois ◽  
Samuel Nibouche ◽  
Philippe Letourmy ◽  
...  
Keyword(s):  
Yellow Leaf ◽  
Reunion Island ◽  
Melanaphis Sacchari ◽  
Sugarcane Yellow Leaf Virus ◽  
Réunion Island ◽  
Tissue Blot Immunoassay ◽  
Resistance To Infection ◽  
Leaf Virus ◽  
Leaf Symptoms

Two experiments, one in Guadeloupe and one in Réunion Island, were performed to transmit different genotypes of Sugarcane yellow leaf virus (SCYLV) to eight sugarcane cultivars differing in resistance to infection by the virus and to yellow leaf. Transmission was attempted from SCYLV-infected sugarcane plants or leaves to healthy tissue-cultured plantlets grown in vitro and with the aphid vector Melanaphis sacchari. After inoculation and elimination of insects with an insecticide, plantlets were transferred to Montpellier, France and grown in a greenhouse. Plants were tested for presence of SCYLV by tissue-blot immunoassay and reverse-transcription polymerase chain reaction after 5 to 6 months of growth. SCYLV genotypes BRA-PER, CUB, and REU were detected in 47, 62, and 39% of plants inoculated with these genotypes in Guadeloupe, respectively. SCYLV genotypes BRA-PER and REU and a mixed infection of genotypes BRA-PER and REU were detected in 56, 33, and 42% of plants inoculated with these genotypes in Réunion Island, respectively. Genotypes BRA-PER and CUB could be transmitted to all eight sugarcane cultivars, but genotype REU could never be transmitted to resistant sugarcane cvs. H78-4153 and H78-3567. SCYLV genotype REU was transmitted successfully to sugarcane cv. R570 in Guadeloupe, but not in Réunion Island. Genotypes BRA-PER and CUB induced yellow leaf symptoms in susceptible or highly susceptible sugarcane cultivars, whereas genotype REU induced very few symptoms. SCYLV was not found in several symptomatic plants, suggesting an association of disease with undetectable populations of the virus or a nonviral cause. This is the first report of variation in infection capacity and in virulence of SCYLV.

Sugarcane yellow leaf virus. [Distribution map].

2018 ◽  
Author(s):  
Keyword(s):  
Tamil Nadu ◽  
Andhra Pradesh ◽  
Uttar Pradesh ◽  
Saccharum Officinarum ◽  
Yellow Leaf ◽  
Distribution Map ◽  
Sugarcane Yellow Leaf Virus ◽  
Leaf Virus ◽  
Virus Distribution ◽  
New Distribution

Abstract A new distribution map is provided for Sugarcane yellow leaf virus. Luteoviridae: Polerovirus. Hosts: sugarcane (Saccharum officinarum), barley (Hordeum vulgare), sorghum (Sorghum bicolor). Information is given on the geographical distribution in Asia (China, Fujian, Guangdong, Guangxi, Guizhou, Hainan, Jiangxi, Yunnan, India, Andhra Pradesh, Bihar, Haryana, Karnataka, Kerala, Madhya Pradesh, Maharashtra, Punjab, Tamil Nadu, Uttar Pradesh, Uttarakhand, Indonesia, Malaysia, Philippines, Sri Lanka, Taiwan, Thailand), Africa (Egypt, Kenya, Mauritius, Reunion, South Africa, Tunisia), North America (USA, Florida, Hawaii, Louisiana, Texas), Central America & Caribbean (Barbados, Costa Rica, Cuba, Guadeloupe, Guatemala, Martinique, Nicaragua, Puerto Rico), South America (Argentina, Brazil, Colombia, Ecuador, Peru, Venezuela).

scholarly journals Genome-wide approaches for the identification of markers and genes associated with sugarcane yellow leaf virus resistance

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Ricardo José Gonzaga Pimenta ◽  
Alexandre Hild Aono ◽  
Roberto Carlos Villavicencio Burbano ◽  
Alisson Esdras Coutinho ◽  
Carla Cristina da Silva ◽  
...  
Keyword(s):  
Virus Resistance ◽  
Machine Learning Algorithms ◽  
Energy Crop ◽  
Yellow Leaf ◽  
Sugarcane Yellow Leaf Virus ◽  
Association Analyses ◽  
Sugarcane Breeding ◽  
Genetic Base ◽  
Genome Wide ◽  
Leaf Virus

AbstractSugarcane yellow leaf (SCYL), caused by the sugarcane yellow leaf virus (SCYLV) is a major disease affecting sugarcane, a leading sugar and energy crop. Despite damages caused by SCYLV, the genetic base of resistance to this virus remains largely unknown. Several methodologies have arisen to identify molecular markers associated with SCYLV resistance, which are crucial for marker-assisted selection and understanding response mechanisms to this virus. We investigated the genetic base of SCYLV resistance using dominant and codominant markers and genotypes of interest for sugarcane breeding. A sugarcane panel inoculated with SCYLV was analyzed for SCYL symptoms, and viral titer was estimated by RT-qPCR. This panel was genotyped with 662 dominant markers and 70,888 SNPs and indels with allele proportion information. We used polyploid-adapted genome-wide association analyses and machine-learning algorithms coupled with feature selection methods to establish marker-trait associations. While each approach identified unique marker sets associated with phenotypes, convergences were observed between them and demonstrated their complementarity. Lastly, we annotated these markers, identifying genes encoding emblematic participants in virus resistance mechanisms and previously unreported candidates involved in viral responses. Our approach could accelerate sugarcane breeding targeting SCYLV resistance and facilitate studies on biological processes leading to this trait.

Movement of aphid-transmitted Sugarcane yellow leaf virus (ScYLV) within and between sugarcane plants

2006 ◽  
Vol 0 (0) ◽  
pp. 070130054450001-???
Author(s):  
A. T. Lehrer ◽  
S. Schenck ◽  
S.-L. Yan ◽  
E. Komor
Keyword(s):  
Yellow Leaf ◽  
Sugarcane Yellow Leaf Virus ◽  
Leaf Virus
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