First Report of Tobacco streak virus Infecting Kenaf (Hibiscus cannabinus) in India

B. V. Bhaskara Reddy; Y. Sivaprasad; C. V. M. Naresh Kumar; A. Sujitha; K. Raja Reddy; D. V. R. Sai Gopal

doi:10.1007/s13337-012-0061-8

First Report of Tobacco Streak Virus Infecting Summer Squash in Georgia, U.S.A

Plant Disease ◽

10.1094/pdis-12-18-2232-pdn ◽

2019 ◽

Vol 103 (6) ◽

pp. 1442 ◽

Cited By ~ 1

Author(s):

S. Bag ◽

A. Tabassum ◽

J. Brock ◽

B. Dutta

Keyword(s):

Tobacco Streak Virus ◽

Streak Virus ◽

First Report ◽

Summer Squash

Download Full-text

First Report of Tobacco Streak Virus Infecting Sunflower in Argentina

Plant Disease ◽

10.1094/pdis-04-19-0849-pdn ◽

2019 ◽

Vol 103 (12) ◽

pp. 3290 ◽

Cited By ~ 1

Author(s):

D. Cabrera Mederos ◽

V. Trucco ◽

N. Bejerman ◽

S. Lenardon ◽

F. Giolitti

Keyword(s):

Tobacco Streak Virus ◽

Streak Virus ◽

First Report

Download Full-text

First Report of Blackberry chlorotic ringspot virus in Rubus sp. in the United States

Plant Disease ◽

10.1094/pdis-91-4-0463c ◽

2007 ◽

Vol 91 (4) ◽

pp. 463-463 ◽

Cited By ~ 6

Author(s):

I. E. Tzanetakis ◽

J. D. Postman ◽

R. R. Martin

Keyword(s):

United States ◽

The United States ◽

Ringspot Virus ◽

Tobacco Streak Virus ◽

Rapid Decline ◽

Streak Virus ◽

Black Raspberry ◽

Rt Pcr ◽

Indicator Plants ◽

First Report

Blackberry chlorotic ringspot virus (BCRV), genus Ilarvirus, has been found in Rubus sp. in Scotland (2) and rose in the United States (4). The possibility that BCRV infects other hosts in the United States was explored. We tested 18 accessions of Fragaria sp. and 30 of Rubus sp. maintained at the National Clonal Germplasm Repository in Corvallis, OR. Ilarviruses had been detected in these plants by reverse transcription (RT)-PCR, ELISA, or had caused symptoms typical of ilarviruses on indicator plants. The accessions were tested by RT-PCR with primers F (5′-GTTTCCTGTGCTCCTCA-3′) and R (5′-GTCACACCGAGGTACT-3′) (4) that amplify a 519 to 522 nt (depending on the isolate) region of the RNA 3 of BCRV. The virus was detected in two accessions of black raspberry (Rubus occidentalis L.): RUB433, cv. Lowden and RUB 9012, cv. New Logan. The sequences of the fragments amplified from these accessions (GenBank Accession Nos. EF041817 and EF041818, respectively) had 97% nt sequence identity to each other and 95 and 88% nt identity to the rose and Scottish isolates (GenBank Accession Nos. DQ329378 and DQ091195, respectively). Chenopodium quinoa indicator plants inoculated with isolate RUB 433 developed mild chlorotic spots on the inoculated leaves 4 days after inoculation. RT-PCR and sequencing of the amplicons verified BCRV infection of C. quinoa. RUB 9012 was used for the characterization of Black raspberry latent virus (BRLV), later thought to be an isolate of Tobacco streak virus (TSV). This accession was recently found to be infected with Strawberry necrotic shock virus (SNSV) but not TSV (3). It is possible that BRLV may be a mixture of SNSV and BCRV. SNSV is one of the most abundant viruses of Rubus sp. in the Pacific Northwest (1), and the finding of another ilarvirus, BCRV, may account in part for the rapid decline of Rubus sp. observed in several fields in Oregon and Washington. To our knowledge, this is the first report of BCRV infecting Rubus sp. outside the United Kingdom. References: (1) A. B. Halgren. Ph.D. Diss. Oregon State University, Corvallis, OR, 2006. (2) A. T. Jones et al. Ann. Appl. Biol. 149:125, 2006. (3) I. E. Tzanetakis et al. Arch. Virol. 149:2001, 2004. (4) I. E. Tzanetakis et al. Plant Pathol. 55:568, 2006.

Download Full-text

First Report of Strawberry necrotic shock virus in China

Plant Disease ◽

10.1094/pdis-02-11-0121 ◽

2011 ◽

Vol 95 (9) ◽

pp. 1198-1198 ◽

Cited By ~ 4

Author(s):

L. Li ◽

H. Yang

Keyword(s):

Nucleic Acid ◽

Home Garden ◽

Tobacco Streak Virus ◽

Chinese Isolate ◽

Streak Virus ◽

Total Nucleic Acid ◽

Rt Pcr ◽

Viral Pathogen ◽

First Report ◽

Strawberry Vein Banding Virus

Strawberry necrotic shock virus (SNSV) is an economically important viral pathogen that infects Fragaria and Rubus spp. SNSV was first identified in the 1950s and early studies indicated that SNSV was a strain of Tobacco streak virus (TSV). Recently, it was shown that SNSV was a distinct virus based on molecular characterization (2). Currently, SNSV is a tentative member of the Ilarvirus genus in the Bromoviridae family. In 2008, a small sampling survey for SNSV was done in Heilongjiang Province of China, and 15 strawberry samples were collected from symptomless strawberries in a home garden that had more than 5 years of strawberry cultivation history. Total nucleic acid was extracted from strawberry leaflets by modified cetyltrimethylammoniumbromide methods (3). Reverse transcription (RT)-PCR was operated with the published primer pair CPbeg F/CPend R (2). Amplified DNA fragments with the predicted size were obtained only in one strawberry sample, which was further cloned and sequenced. The sequence (GenBank Accession No. HQ830017) was closely related and highly homologous (89.7 to 98.5% identity) to that of viral isolates (GenBank Accession Nos. AY363228-AY363242) from Fragaria and Rubus spp. Phylogenetic analysis based on nucleotide sequence of the coat protein gene was done with the neighbor-joining method of MEGA 4.0 software. The result showed that all the isolates of SNSV fell into two distinct clades. The Chinese isolate formed one small clade with Japanese isolate 1291. The isolate was also transmitted to Chenopodium quinoa by mechanical inoculation in the greenhouse, and the symptom of chlorotic mottling could be found in C. quinoa and detected by RT-PCR. To determine whether the sample was infected by other strawberry viruses, RT-PCR assays with the published primer pairs SVBVdeta/SVBVdetb, SMoVdeta/SMoVdetb, and SMYEVdeta/SMYEVdetb were also performed for detection of Strawberry vein banding virus, Strawberry mottle virus, and Strawberry mild yellow edge virus using total nucleic acid extracted from the SNSV-positive sample as a template (1). The result indicated that it had been also infected by Strawberry mild yellow edge virus, although no visible symptoms were observed. To our knowledge, this is the first report of SNSV in strawberry in China. Additional work is needed to elucidate the biological characterization and significance of the finding. References: (1) J. R. Thompson et al. J. Virol. Methods 111:85, 2003. (2) I. E. Tzanetakis et al. Arch. Virol. 149:2001, 2004. (3) H. Y. Yang et al. Acta Hortic. 764:127, 2007.

Download Full-text

Identification of Tobacco streak virus Associated With a Virus-like Mottle Symptom on Hosta

Plant Health Progress ◽

10.1094/php-2013-122-01-br ◽

2013 ◽

Vol 14 (1) ◽

pp. 60 ◽

Cited By ~ 1

Author(s):

John R. Fisher

Keyword(s):

United States ◽

Capsid Protein ◽

Movement Protein ◽

The United States ◽

Tobacco Streak Virus ◽

Streak Virus ◽

First Report ◽

Leaf Symptom

In this brief, the authors describe the identification of Tobacco streak virus associated with a virus-like leaf symptom on Hosta spp. The identity of the virus was confirmed by ELISA and PCR using cDNAs synthesized from dsRNA template and immuncaptured virions and primers to amplify the movement protein and capsid protein genes. This is the first report of TSV infecting Hosta in Ohio, and to our knowledge in the United States. Accepted for publication 17 December 2012. Published 22 January 2013.

Download Full-text

First report of natural infection of Capsicum annuum by Tobacco streak virus in India

Plant Pathology ◽

10.1111/j.1365-3059.2005.01127.x ◽

2005 ◽

Vol 54 (2) ◽

pp. 257-257 ◽

Cited By ~ 11

Author(s):

R. K. Jain ◽

S. Bag ◽

L. P. Awasthi

Keyword(s):

Capsicum Annuum ◽

Natural Infection ◽

Tobacco Streak Virus ◽

Streak Virus ◽

First Report

Download Full-text

First Report of Tobacco Streak Virus Infecting Sunn Hemp (Crotalaria juncea) in Hawaii

Plant Disease ◽

10.1094/pdis-04-18-0697-pdn ◽

2018 ◽

Vol 102 (12) ◽

pp. 2664

Author(s):

A. Kong ◽

M. Long ◽

A. Arakaki ◽

M. Melzer

Keyword(s):

Tobacco Streak Virus ◽

Streak Virus ◽

Crotalaria Juncea ◽

First Report ◽

Sunn Hemp

Download Full-text

First Report of Columnea latent viroid (CLVd) in Tomato in Mali

Plant Disease ◽

10.1094/pdis-10-12-0920-pdn ◽

2013 ◽

Vol 97 (5) ◽

pp. 692-692 ◽

Cited By ~ 10

Author(s):

O. Batuman ◽

R. L. Gilbertson

Keyword(s):

Mosaic Virus ◽

Tobacco Streak Virus ◽

Streak Virus ◽

Rt Pcr ◽

First Report ◽

Stunted Growth ◽

Tomato Seedlings ◽

Leaf Veins ◽

Dna Fragment ◽

Columnea Latent Viroid

During surveys of tomato (Solanum lycopersicum) fields in Niono, Mali, conducted in March 2011, unusual disease symptoms, including stunted growth, epinasty, and chlorosis of leaves and necrosis of leaf veins and stems were observed in multiple fields. The incidence of these symptoms was low (~1 to 5%), but they were distinct from those associated with known diseases in the region. A representative leaf sample with these symptoms was applied to filter paper (FTA cards, Whatman), and DNA and RNA extracts were prepared according to manufacturer instructions. RT-PCR tests for Tomato spotted wilt virus, Tobacco streak virus, Tomato necrotic spot virus, Tobacco/tomato mosaic viruses, Cucumber mosaic virus, Alfalfa mosaic virus, torradoviruses, and potyviruses, and PCR tests for begomoviruses, phytoplasmas, and ‘Candidatus Liberibacter’ infection were also negative. However, virus-like symptoms developed in all 16 tomato seedlings (cv. Early Pak 7) 7 to 10 days after mechanical (sap) inoculation with inoculum prepared from the FTA sample. No symptoms developed in mock-inoculated control plants (n = 3). Symptoms induced included stunted growth and severe epinasty of leaves, followed by necrosis of leaf veins, petioles, and stems. These symptoms were similar to those observed in plants in Mali. When RNA extracts prepared from leaves of these symptomatic plants were mechanically inoculated onto 24 tomato seedlings, similar symptoms developed in all plants, suggesting the causal agent might be a viroid. RT-PCR tests with RNA from symptomatic tomato leaves and universal (3) and various specific Pospiviroid primer pairs were negative. However, equivalent RT-PCR tests conducted with the pCLV4/pCLVR4 primer pair specific for Columnea latent viroid (CLVd) (2) generated a DNA fragment of the expected size (~370 bp). The sequence of this DNA fragment (GenBank Accession No. JQ362419) was 99% identical with those of CLVd isolates from the Netherlands (AY373446 and AY372396). In host range studies, the CLVd isolate from Mali induced symptoms in all 48 mechanically-inoculated tomato plants, whereas no symptoms developed (up to 90 days after inoculation) in inoculated Chenopodium quinoa, C. amaranticolor, Nicotiana benthamiana, N. tabacum (cvs. Havana, Glurk and Turkish), N. glutinosa, Datura stramonium, common bean (cvs. Topcrop and Pinto bean), pumpkin (cv. Small Sugar), pepper (Capsicum annuum, cv. Yolo Wonder) and cucumber (cvs. Emparator and Poinsett 76) plants (results of three independent experiments with six plants per experiment). Symptomless infections were detected in pepper (24 of 30), N. benthamiana (25 of 25), and N. tabacum cv. Turkish (11 of 24) plants by RT-PCR with the pCLV4/pCLVR4 primer pair. To our knowledge, this is the first report of CLVd infecting tomato in Mali. RT-PCR tests of seeds collected from CLVd-infected tomato, pepper, and N. benthamiana plants also detected CLVd (1). Thus, it is possible that CLVd was introduced into Mali in association with seed. References: (1) O. Batuman and R. L. Gilbertson. Phytopathology 102:S4.9, 2012. (2) R. L. Spieker. Arch. Virol. 141:1823, 1996. (3) J. T. J. Verhoeven et al. Eur. J. Plant Pathol. 110:823, 2004.

Download Full-text

First Report of Tobacco streak virus Infecting Safflower (Carthamus tinctorius) in Maharashtra, India

Plant Disease ◽

10.1094/pdis.2003.87.11.1396b ◽

2003 ◽

Vol 87 (11) ◽

pp. 1396-1396 ◽

Cited By ~ 2

Author(s):

S. Chander Rao ◽

R. D. V. J. Prasada Rao ◽

V. Manoj Kumar ◽

Divya S. Raman ◽

M. A. Raoof ◽

...

Keyword(s):

Carthamus Tinctorius ◽

Tobacco Streak Virus ◽

Enzyme Linked Immunosorbent Assay ◽

Streak Virus ◽

Leaf Extracts ◽

Systemic Necrosis ◽

First Report ◽

Veinal Necrosis ◽

Terminal Bud ◽

Leaf Necrosis

Safflower, Carthamus tinctorius L. (Asteraceae), is extensively cultivated in India, China, and other parts of Asia for edible oil, dyeing agent, and its medicinal value. In 2003, safflower entry (NARI-6) in the All-India Coordinated Research Project on Oilseeds (Safflower) grown in the experimental fields of M/s Syngenta India Ltd., Aurangabad (Maharashtra State, India) exhibited symptoms of veinal and leaf necrosis, necrotic streaks on the stem, necrosis of the terminal bud, and ultimately plant death. The disease was attributed to Tobacco streak virus (TSV) because sunflower growing adjacent to safflower showed similar symptoms caused by TSV (1). Mechanical inoculations of sap from symptomatic safflower leaves caused typical symptoms of TSV (local, irregular, necrotic rings, veinal necrosis, and systemic veinal necrosis) on Vigna unguiculata (L.) Walp. cv. C-152 and Phaseolus vulgaris (L.) cv. Topcrop, and symptoms of local, necrotic lesions, veinal necrosis, and systemic necrosis of leaf and growing bud on Arachis hypogaea L. cv. JL-24. Sap-inoculated safflower cv. Manjeera showed chlorotic and necrotic local lesions followed by systemic leaf necrosis, leading to necrosis and death of the terminal bud. Safflower cvs. A-1, BIP-2, Co-1, and Bheema (10 plants of each cultivar) inoculated with sap from safflower plants showing typical TSV symptoms did not show any visible symptoms except stunting, but six to nine plants of each cultivar tested positive for TSV using enzyme-linked immunosorbent assay (ELISA) tests. In direct antigen coating-ELISA, the virus reacted positively with antiserum produced to an isolate of TSV from peanut (2) and to antiserum to TSV (ATCC-PVAS 276 for Datura stramonium), but did not react to peanut bud necrosis tospovirus antiserum. Examination of leaf extracts using leaf-dips and immunosorbent electron microscopy with the antiserum of TSV-peanut isolate showed isometric particles resembling those in the genus Ilarvirus. To our knowledge, this is the first report of an isolate of Tobacco streak virus infecting safflower. References: (1) R. D. V. J. Prasada Rao et al. J. Oilseeds Res. 17:400, 2000. (2) A. S. Reddy et al. Plant Dis. 86:173, 2002.

Download Full-text

First Report of Bean pod mottle virus in Soybean in Canada

Plant Disease ◽

10.1094/pdis.2002.86.3.330a ◽

2002 ◽

Vol 86 (3) ◽

pp. 330-330 ◽

Cited By ~ 6

Author(s):

R. Michelutti ◽

J. C. Tu ◽

D. W. A. Hunt ◽

D. Gagnier ◽

T. R. Anderson ◽

...

Keyword(s):

Soybean Mosaic Virus ◽

Mottle Virus ◽

Tobacco Streak Virus ◽

Enzyme Linked Immunosorbent Assay ◽

Streak Virus ◽

Growth Stages ◽

Essex County ◽

Bean Pod Mottle Virus ◽

First Report ◽

Soybean Plants

In 2001, soybean fields were surveyed to determine the incidence of viruses because soybean aphids (Aphis glycines Matsamura), known to transmit Soybean mosaic virus (SMV) (2), were found in Ontario. In addition, bean leaf beetle (Cerotoma trifurcata Forster) was found during 2000 to be contaminated with Bean pod mottle virus (BPMV), although soybean plants, on which the beetles were feeding, tested negative (3). In the current survey, young soybean leaves were selected at random in July and August from 20 plants per site at growth stages R4 to R5 (1) from 415 sites representing the entire soybean-producing area in Ontario. Samples were maintained under cool conditions until received at the laboratory, where they were promptly processed. A combined sub-sample was obtained from the 20 plants per site. The 415 sub-samples were tested for SMV, BPMV, Tobacco ringspot virus (TRSV), and Tobacco streak virus (TSV) using polyclonal antibody kits for double-antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA) (Agdia Inc., Elkart, IN). The ELISA plates were read with a plate reader (MRX, Dynex Technologies Inc., Chantilly, VA), and results were analyzed using ELISA software (Leading Edge Research, Merrickville, Ontario) and compared positive and negative controls (Agdia). TRSV was detected in one sample from Essex County and another sample from Middlesex County. SMV, BPMV, and TSV were not found in commercial soybean fields. However, SMV and BPMV were found in samples originating from two soybean breeding nurseries, one in Essex County and one in Kent County. Seedlings of soybean cv. Williams 82 were inoculated in the greenhouse with sap from leaf samples that tested positive for BPMV. Leaves of plants that developed mosaic symptoms were retested using ELISA and confirmed to be positive for BPMV. SMV and TRSV have been found previously in commercial soybean fields in Ontario (4). To our knowledge, this is the first report of BPMV on soybean plants in Canada. References: (1) W. R. Fehr et al. Merr. Crop. Sci. 11:929, 1971. (2) J. H. Hill et al. Plant Dis. 85:561, 2001. (3) A. U. Tenuta. Crop Pest. 5 (11):8, 2000. (4) J. C. Tu. Can. J. Plant Sci. 66:491, 1986.

Download Full-text