Enhancement of undecylprodigiosin production from marine endophytic recombinant strain Streptomyces sp. ALAA-R20 through low-cost induction strategy

2021 ◽  
Author(s):  
Nourah Hassan Alzahrani ◽  
Alaa Ahmed Mohamed El-Bondkly ◽  
Mervat Morsy Abbas Ahmed El-Gendy ◽  
Ahmed Mohamed El-Bondkly
Keyword(s):  
Recombinant Strain ◽  
Low Cost ◽  
Streptomyces Sp ◽  
Induction Strategy

scholarly journals Antibiotics production in optimized culture condition using low cost substrates from Streptomyces sp. AS4 isolated from mangrove soil sediment

2020 ◽  
Vol 32 (2) ◽  
pp. 1528-1535 ◽  
Author(s):  
Dunia A. Al Farraj ◽  
Rakesh Varghese ◽  
Csaba Vágvölgyi ◽  
Mohamed Soliman Elshikh ◽  
A.M. Alokda ◽  
...  
Keyword(s):  
Culture Condition ◽  
Low Cost ◽  
Streptomyces Sp ◽  
Mangrove Soil ◽  
Soil Sediment

scholarly journals Use of a Recombinant Strain of Mycobacterium avium Expressing β-Galactosidase To Evaluate the Activities of Antimycobacterial Agents inside Macrophages

2001 ◽  
Vol 45 (1) ◽  
pp. 356-358 ◽  
Author(s):  
Giuseppantonio Maisetta ◽  
Giovanna Batoni ◽  
Manuela Pardini ◽  
Antonella Boschi ◽  
Daria Bottai ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Mycobacterium Avium ◽  
Recombinant Strain ◽  
Antimicrobial Agents ◽  
Low Cost ◽  
Rapid Screening ◽  
Murine Macrophages ◽  
Antimycobacterial Agents

ABSTRACT A reliable and low-cost method that enables rapid screening of the activity exerted by new antimicrobial agents on intracellularly growingMycobacterium avium has been developed. To this aim, a recombinant (lacZ) strain of M. aviumexpressing the Escherichia coli β-galactosidase gene was used to evaluate, in murine macrophages, the susceptibility of M. avium to common antimycobacterial agents. β-Galactosidase levels, measured in the presence of each of the antibiotics tested, were closely correlated with the number of CFU recovered from theM. avium lacZ strain-infected macrophages.

scholarly journals Production of streptomycete inoculum in sterilized rice

2007 ◽  
Vol 64 (6) ◽  
pp. 641-644 ◽  
Author(s):  
Ana Cristina Fermino Soares ◽  
Carla da Silva Sousa ◽  
Marlon da Silva Garrido ◽  
Jane Oliveira Perez
Keyword(s):  
Plant Disease ◽  
Growth Promotion ◽  
Low Cost ◽  
Field Work ◽  
Alternative Substrate ◽  
Streptomyces Species ◽  
Streptomyces Sp ◽  
Plant Disease Control ◽  
Counting Chamber ◽  
Develop Technology

Actinomycetes are important plant disease control and growth promotion agents, which makes it necessary to develop technology to produce large quantities of inoculum for green-house and field work. The present study had the objective of evaluating the growth of several isolates of Streptomyces in sterile rice for inoculum production. The sterile rice was inoculated with isolates of S. thermotolerans, S. griseus subsp. griseus, Streptomyces sp. N0035, S. purpurascens, and Streptomyces sp., and incubated at 28 ± 2ºC. Five days after its inoculation, mycelial growth and sporulation was observed for all Streptomyces isolates on the rice grains. Twelve days after incubation, the colonized rice was transferred to envelopes of dark brown paper and let to dry in an incubator at 30ºC for three days. After drying, 1g of colonized rice was added to 200 mL of sterile distilled water and the number of spores was counted under a microscope with a Newbauer counting chamber. Spore production varied from 0.14 × 10(9) to 1.47 × 10(9) spores per gram of rice and differed among the Streptomyces species. Sterile rice can be an alternative substrate for low cost mass production of Streptomyces inoculum.

scholarly journals Medium Formulation and its Optimization to Enhance Protease Production by Streptomyces sp. Isolated from Mangroves

2018 ◽  
Vol 15 (3) ◽  
pp. 719-728 ◽  
Author(s):  
M. Parthasarathy ◽  
J. Joel Gnanadoss
Keyword(s):  
Sodium Nitrate ◽  
Tamil Nadu ◽  
Low Cost ◽  
Fermentation Medium ◽  
Protease Production ◽  
New Approach ◽  
Streptomyces Sp ◽  
Protease Enzyme ◽  
Medium Formulation ◽  
Important Enzyme

Streptomyces sp. LCJ12A was isolated from the soil and sediments of Pichavaram Mangrove Forest, Tamil Nadu, India. Production of protease from the Streptomyces sp. LCJ12A was carried out by using submerged fermentation. To enhance the protease production, the fermentation medium was formulated and optimized. Different carbon, nitrogen and inducer sources were used for the optimization. In that fructose, sodium nitrate and red gram husk showed greater quantity of protease production and their different concentrations were optimized in Protease production broth (PPB). Response surface methodology (RSM) was employed for the medium optimization at a low cost for the production of industrially important enzyme. The optimized values showed that fructose at 2.0 g/L enhances the yield of protease up to 120.08±2.2 U/mL, sodium nitrate at 2.0 g/L maximize the protease production up to 180.35±1.9 U/mL and red gram husk at 2.0 g/L yields 194.16±2.2 U/mL which was 1.6 times higher when compared to the unoptimized medium. Statistical optimization by using RSM predicted that 327.16 U/mL of protease enzyme can be produced. Through experimentation based on RSM, the protease yield reached up to 323.4 U/mL. When compared to unoptimized medium, the statistically optimized medium produced 3 times higher yield. As a result of the optimization studies, an increase in protease activity was reached compared to the unoptimized conditions and thus offers a new approach for industrial enzyme production.

scholarly journals Development and Field Performance of a Broad-Spectrum Nonviable Asporogenic Recombinant Strain of Bacillus thuringiensis with Greater Potency and UV Resistance

1999 ◽  
Vol 65 (9) ◽  
pp. 4032-4039 ◽  
Author(s):  
Vincent Sanchis ◽  
Michel Gohar ◽  
Josette Chaufaux ◽  
Olivia Arantes ◽  
Alain Meier ◽  
...  
Keyword(s):  
Bacillus Thuringiensis ◽  
Recombinant Strain ◽  
European Corn Borer ◽  
Low Cost ◽  
Tobacco Budworm ◽  
Beet Armyworm ◽  
Cotton Leaf ◽  
Uv Degradation ◽  
Corn Borer ◽  
Activity Spectrum

ABSTRACT The main problems with Bacillus thuringiensis products for pest control are their often narrow activity spectrum, high sensitivity to UV degradation, and low cost effectiveness (high potency required). We constructed a sporulation-deficient SigK− B. thuringiensis strain that expressed a chimericcry1C/Ab gene, the product of which had high activity against various lepidopteran pests, including Spodoptera littoralis (Egyptian cotton leaf worm) and Spodoptera exigua (lesser [beet] armyworm), which are not readily controlled by other Cry δ-endotoxins. The SigK− host strain carried the cry1Ac gene, the product of which is highly active against the larvae of the major pests Ostrinia nubilalis (European corn borer) and Heliothis virescens (tobacco budworm). This new strain had greater potency and a broader activity spectrum than the parent strain. The crystals produced by the asporogenic strain remained encapsulated within the cells, which protected them from UV degradation. Thecry1C/Ab gene was introduced into the B. thuringiensis host via a site-specific recombination vector so that unwanted DNA was eliminated. Therefore, the final construct contained no sequences of non-B. thuringiensis origin. As the recombinant strain is a mutant blocked at late sporulation, it does not produce viable spores and therefore cannot compete with wild-typeB. thuringiensis strains in the environment. It is thus a very safe biopesticide. In field trials, this new recombinant strain protected cabbage and broccoli against a pest complex under natural infestation conditions.

Magnesium-induced preparation of boron nitride nanotubes and their application in thermal interface materials

Nanoscale ◽  
2019 ◽  
Vol 11 (24) ◽  
pp. 11457-11463 ◽  
Author(s):  
Chaowei Li ◽  
Xiaoyang Long ◽  
Songfeng E ◽  
Qichong Zhang ◽  
Taotao Li ◽  
...  
Keyword(s):  
Boron Nitride ◽  
Low Cost ◽  
Boron Nitride Nanotubes ◽  
Thermal Interface Materials ◽  
Thermal Interface ◽  
Induction Strategy ◽  
Interface Materials

The Mg induction strategy is low cost and efficiently generates BNNTs, which can be widely used in thermal interface materials.

Promotion of feather waste recycling by enhancing reducing power and keratinase activity of Streptomyces sp. SCUT-3

2021 ◽  
Author(s):  
Shuang Liang ◽  
Jun-Jin Deng ◽  
Ming-Shu Zhang ◽  
Zi-Yang Luo ◽  
Wen-Jun Lu ◽  
...  
Keyword(s):  
Low Cost ◽  
Reducing Power ◽  
Waste Recycling ◽  
Streptomyces Sp ◽  
Feather Waste ◽  
Poultry Farming

As a potential renewable and nitrogen-rich resource, millions of tons of feather waste are generated from poultry farming, which has been steadily increasing. Bioconversion is the most promising low-cost and...

scholarly journals O POTENCIAL DO STREPTOMYCES ISOLADOS NA REGIÃO DE MAUÉS NA PRODUÇÃO DE ENZIMAS HIDROLÍTICAS

2017 ◽  
Vol 39 (2) ◽  
pp. 202
Author(s):  
Leila Viviane Araujo Coelho ◽  
Camila Beatriz Atanásio Borba ◽  
Erik Jonne Vieira de Melo ◽  
Leonor Alves de Oliveira Da Silva
Keyword(s):  
Enzyme Production ◽  
Specific Activity ◽  
Low Cost ◽  
Hydrolytic Enzymes ◽  
Industrial Biotechnology ◽  
Streptomyces Sp ◽  
Brazil Nuts ◽  
Production Area ◽  
Semi Solid ◽  
Micro Organisms

The enzymes of microbial production area has grown in the last decades due to use in various industrial fields, and with that the research needs to innovations of existing products. The hydrolytic enzymes are biological catalysts of great commercial importance for low cost and high availability. This enzyme class has received special attention about his versatility in several areas of industrial biotechnology. The Brazil-nuts is a possible source of nutrients for the growth of micro-organisms in search of effective enzyme production and low cost. Were used two strains of Streptomyces sp. or production of protease, lipase, amylase and alkaline phosphatase using the residue Brazil-nut as carbon source in fermentation, semi-solid and liquid for the medium ISP-2 at pH 7.2 at a temperature 37°C for 15 days. The results showed that the best enzymatic activity was to protease secreted by Streptomyces sp. (1N) in semi solid fermentation. Concomitantly, was carried out an enzyme production kinetics with peak activity of 0.196 U / ml activity for 144 hours. The specific activity was observed a value of 2.17 U / mg protein for 240 hours. The production of enzymes excreted by Streptomyces sp. using the residue of Brazil-nuts is a resource for obtaining enzymes with high potential for solid state fermentation.

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