scholarly journals Detection and Site Localization of Phosphorylcholine-Modified Peptides by NanoLC-ESI-MS/MS Using Precursor Ion Scanning and Multiple Reaction Monitoring Experiments

2014 ◽  
Vol 26 (3) ◽  
pp. 460-471 ◽  
Author(s):  
Thomas Timm ◽  
Christof Lenz ◽  
Dietrich Merkel ◽  
Christian Sadiffo ◽  
Julia Grabitzki ◽  
...  
Keyword(s):  
Multiple Reaction Monitoring ◽  
Reaction Monitoring ◽  
Esi Ms ◽  
Modified Peptides ◽  
Site Localization ◽  
Precursor Ion Scanning

Sensitive Determination of C-Alkylated Flavonoids by HPLC-ESI-MS/MS Using Multiple Reaction Monitoring Approach: Pseudarthria hookeri as a Case Study

2019 ◽  
Vol 57 (10) ◽  
pp. 944-949
Author(s):  
Ina Faraz ◽  
Arslan Ali ◽  
Faraz Ul Haq ◽  
Joseph Tchamgoue ◽  
Simeon F Kouam ◽  
...  
Keyword(s):  
Quality Control ◽  
Method Development ◽  
Control Method ◽  
Raw Materials ◽  
Multiple Reaction Monitoring ◽  
Herbal Medicines ◽  
Reaction Monitoring ◽  
Linear Calibration ◽  
Esi Ms ◽  
Plant Raw Materials

Abstract One of the major problems with the formulation of herbal medicines is the quality control of plant material to ensure its efficacy and safety. Quality control of medicinal plants requires analysis of many bioactive compounds present in the plant. C-alkylated flavonoids are an important bioactive subclass of flavonoids. A simple, rapid, sensitive and selective method is presented here for the quantification of bioactive C-alkylated flavonoids. This is the first quantitative method for analysis of C-alkylated flavonoids based on the multiple reaction monitoring (MRM) approach so far. This study focuses on method development for quantification of bioactive C-alkylated flavonoids. Quantification of a total of five C-alkylated flavonoids was done employing the MRM approach on an HPLC-QqQ-MS instrument. LODs and LOQs for quantified flavonoids were in the range of 0.41–1.32 and 1.23–3.96 ng/mL, respectively. Linear calibration curves between 25 and 1500 ng/mL were obtained with the regression coefficients of ≥0.996. Accuracy (% bias) and precision (% RSD) of the analyses were found to be less than 5%. Developed HPLC-ESI-MS/MS can be employed as a quality control method of plant raw materials.

scholarly journals Quantification of seminolipid by LC-ESI-MS/MS-multiple reaction monitoring: compensatory levels inCgtmice

2010 ◽  
Vol 51 (12) ◽  
pp. 3548-3558 ◽  
Author(s):  
Kessiri Kongmanas ◽  
Hongbin Xu ◽  
Arman Yaghoubian ◽  
Laura Franchini ◽  
Luigi Panza ◽  
...  
Keyword(s):  
Multiple Reaction Monitoring ◽  
Reaction Monitoring ◽  
Esi Ms

Quantification of the Polyisoprenylated Benzophenones Garcinol and Isogarcinol Using Multiple Reaction Monitoring LC/Electrospray Ionization-MS/MS Analysis of Ultrasound-Assisted Extracts of Garcinia indica Fruits

2014 ◽  
Vol 97 (5) ◽  
pp. 1317-1322 ◽  
Author(s):  
Jaideep Bibishan Bharate ◽  
Ram Ashrey Vishwakarma ◽  
Sandip Bibishan Bharate ◽  
Manoj Kushwaha ◽  
Ajai Prakash Gupta
Keyword(s):  
Electrospray Ionization ◽  
Multiple Reaction Monitoring ◽  
Extraction Procedure ◽  
Extraction Process ◽  
Reaction Monitoring ◽  
Anticancer Compounds ◽  
Esi Ms ◽  
Garcinia Indica ◽  
Highly Sensitive ◽  
Positive Ion

Abstract This paper describes a method that includes an optimized extraction process and identification and quantification of two anticancer compounds (garcinol and isogarcinol) by LC/electrospray ionization (ESI)-MS/MS in the multiple reaction monitoring (MRM) mode. The study aimed to develop a fast, accurate, and sensitive method for the quantification of garcinol and isogarcinol in different extracts of Garcinia indica fruits. The compounds were detected using LC/ESI-MS/MS in the positive-ion mode and quantified in the MRM mode using a transition mass of m/z 603.3/411 taken as the quantifier and 603.3/343.2 as the qualifier for garcinol and isogarcinol. Five point calibration curves were linear in the range of 2 to 10 ng/mL for garcinol and 0.5 to 6 ng/mL for isogarcinol, with a correlation coefficient of ≥0.990 for both. LOQ for garcinol and isogarcinol was 0.06 and 0.05 ng/mL, respectively, while LOD was 0.021 and 0.017 ng/mL respectively. Our work demonstrated optimization of extraction procedure, fast and highly sensitive quantification (pg level LOQ), and validation of the developed method for the investigated compounds in fruit extracts of G. indica.

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