scholarly journals Rapid Screening of Phospholipid Biomarker Candidates from Prostate Cancer Urine Samples by Multiple Reaction Monitoring of UPLC-ESI-MS/MS and Statistical Approaches

2014 ◽  
Vol 35 (4) ◽  
pp. 1133-1138 ◽  
Author(s):  
Sangsoo Lim ◽  
Dae Young Bang ◽  
Koon Ho Rha ◽  
Myeong Hee Moon
Keyword(s):  
Prostate Cancer ◽  
Multiple Reaction Monitoring ◽  
Urine Samples ◽  
Rapid Screening ◽  
Reaction Monitoring ◽  
Esi Ms ◽  
Statistical Approaches

Sensitive Determination of C-Alkylated Flavonoids by HPLC-ESI-MS/MS Using Multiple Reaction Monitoring Approach: Pseudarthria hookeri as a Case Study

2019 ◽  
Vol 57 (10) ◽  
pp. 944-949
Author(s):  
Ina Faraz ◽  
Arslan Ali ◽  
Faraz Ul Haq ◽  
Joseph Tchamgoue ◽  
Simeon F Kouam ◽  
...  
Keyword(s):  
Quality Control ◽  
Method Development ◽  
Control Method ◽  
Raw Materials ◽  
Multiple Reaction Monitoring ◽  
Herbal Medicines ◽  
Reaction Monitoring ◽  
Linear Calibration ◽  
Esi Ms ◽  
Plant Raw Materials

Abstract One of the major problems with the formulation of herbal medicines is the quality control of plant material to ensure its efficacy and safety. Quality control of medicinal plants requires analysis of many bioactive compounds present in the plant. C-alkylated flavonoids are an important bioactive subclass of flavonoids. A simple, rapid, sensitive and selective method is presented here for the quantification of bioactive C-alkylated flavonoids. This is the first quantitative method for analysis of C-alkylated flavonoids based on the multiple reaction monitoring (MRM) approach so far. This study focuses on method development for quantification of bioactive C-alkylated flavonoids. Quantification of a total of five C-alkylated flavonoids was done employing the MRM approach on an HPLC-QqQ-MS instrument. LODs and LOQs for quantified flavonoids were in the range of 0.41–1.32 and 1.23–3.96 ng/mL, respectively. Linear calibration curves between 25 and 1500 ng/mL were obtained with the regression coefficients of ≥0.996. Accuracy (% bias) and precision (% RSD) of the analyses were found to be less than 5%. Developed HPLC-ESI-MS/MS can be employed as a quality control method of plant raw materials.

scholarly journals Quantification of seminolipid by LC-ESI-MS/MS-multiple reaction monitoring: compensatory levels inCgtmice

2010 ◽  
Vol 51 (12) ◽  
pp. 3548-3558 ◽  
Author(s):  
Kessiri Kongmanas ◽  
Hongbin Xu ◽  
Arman Yaghoubian ◽  
Laura Franchini ◽  
Luigi Panza ◽  
...  
Keyword(s):  
Multiple Reaction Monitoring ◽  
Reaction Monitoring ◽  
Esi Ms

Quantification of the Polyisoprenylated Benzophenones Garcinol and Isogarcinol Using Multiple Reaction Monitoring LC/Electrospray Ionization-MS/MS Analysis of Ultrasound-Assisted Extracts of Garcinia indica Fruits

2014 ◽  
Vol 97 (5) ◽  
pp. 1317-1322 ◽  
Author(s):  
Jaideep Bibishan Bharate ◽  
Ram Ashrey Vishwakarma ◽  
Sandip Bibishan Bharate ◽  
Manoj Kushwaha ◽  
Ajai Prakash Gupta
Keyword(s):  
Electrospray Ionization ◽  
Multiple Reaction Monitoring ◽  
Extraction Procedure ◽  
Extraction Process ◽  
Reaction Monitoring ◽  
Anticancer Compounds ◽  
Esi Ms ◽  
Garcinia Indica ◽  
Highly Sensitive ◽  
Positive Ion

Abstract This paper describes a method that includes an optimized extraction process and identification and quantification of two anticancer compounds (garcinol and isogarcinol) by LC/electrospray ionization (ESI)-MS/MS in the multiple reaction monitoring (MRM) mode. The study aimed to develop a fast, accurate, and sensitive method for the quantification of garcinol and isogarcinol in different extracts of Garcinia indica fruits. The compounds were detected using LC/ESI-MS/MS in the positive-ion mode and quantified in the MRM mode using a transition mass of m/z 603.3/411 taken as the quantifier and 603.3/343.2 as the qualifier for garcinol and isogarcinol. Five point calibration curves were linear in the range of 2 to 10 ng/mL for garcinol and 0.5 to 6 ng/mL for isogarcinol, with a correlation coefficient of ≥0.990 for both. LOQ for garcinol and isogarcinol was 0.06 and 0.05 ng/mL, respectively, while LOD was 0.021 and 0.017 ng/mL respectively. Our work demonstrated optimization of extraction procedure, fast and highly sensitive quantification (pg level LOQ), and validation of the developed method for the investigated compounds in fruit extracts of G. indica.

Development of Fast and Simple LC-ESI-MS/MS Technique for the Quantification of Regorafenib; Application to Pharmacokinetics in Healthy Rabbits.

2019 ◽  
Vol 16 ◽  
Author(s):  
Yatha Ravi ◽  
D.V.R.N. Bhikshapathi ◽  
Shankar Cheruku ◽  
Bigala Rajkamal
Keyword(s):  
Mobile Phase ◽  
Ammonium Acetate ◽  
Multiple Reaction Monitoring ◽  
Research Work ◽  
Internal Standard ◽  
Oral Route ◽  
Reaction Monitoring ◽  
Mobile Phase Composition ◽  
Esi Ms ◽  
Bioavailability Study

Background: A simple quantification technique by liquid chromatography–electrospray ionization-tandem mass spectrometry (LC-ESI-MS/MS) is required for regorafenib in biological matrices with bioavailability studies in healthy rabbits, when compared with reported techniques. Objective: The main aim of the research work is to develop a validated LC-ESI-MS/MS technique for the quantification of regorafenib and application to bioavailability studies in healthy rabbits. Methods: Chromatographic separation was achieved with hypersil-C18 analytical column (50mm×4.6 mm, 4µm) and a mobile phase composition of acetonitrile and 5mM ammonium acetate in the proportion of 70:30. The mobile phase was infused into the column with high pressure to get 0.7 ml/min flow rate. The total retention time of the analyte is promising when compared with the existed methods for regorafenib. Quantitation was processed by monitoring transitions of m/z -483.0/262.0 and 450.0/260.0 for regorafenib and internal standard respectively in multiple reaction monitoring. Results: The linearity equation and correlation coefficient (R2) findings were y =0.9948x+2.6624 and 0.998 respectively. The intra and inter-day precision of the developed technique was found between 1.00 – 8.50% for the QC-samples (2, 4, 240 and 480ng/ml). From bioavailability study, the drug was shown Tmax of 3.688 ± 0.754; average AUC0͢α and AUC0͢t was 6476.81 ± 259.59 and 6213.845 ± 257.892 and Cmax was found to be 676.91 ± 22.045 in healthy rabbits. Conclusion: The developed technique was validated and successfully applied in the pharmacokinetic study of drug (40 mg tablet) administered through oral route in healthy rabbits.

Simultaneous detection of phillyrin and its three metabolites in rat bile, excrement and urine via HPLC-ESI-MS/MS quantitative method

2021 ◽  
Vol 17 ◽  
Author(s):  
Xizhe Liu ◽  
Feigao Li ◽  
Lantong Zhang
Keyword(s):  
Multiple Reaction Monitoring ◽  
Simultaneous Detection ◽  
The Body ◽  
Urine Samples ◽  
Negative Ion ◽  
Mass System ◽  
Esi Ms ◽  
Forsythia Suspensa ◽  
Accuracy And Repeatability ◽  
Rat Bile

Introduction: Phillyrin, the main pharmacological component of Forsythia suspensa, exhibits a wide variability of therapeutic activities, such as anti-oxidative stress, free radical-clearing, antibacterial activity, hepatic protection, restoration of endothelial glycocalyx damage, prevention of bone loss, attenuation of inflammatory responses, and so on. Previous research has found that phillyrin is not easily absorbed by the body and is rarely excreted into bile, excrement and urine, suggesting that phillyrin circulates primarily in the form of metabolites. Materials and Methods: In the present study, HPLC-ESI-MS/MS method was used for the simultaneous detection of phillyrin and its three metabolites in rat bile, excrement and urine samples. Liquid-liquid extraction with ethyl acetate was carried out for the pretreatment of bile and urine samples, while excrement samples were subjected to ultrasonic pretreatment with acetone. Chromatographic separation was performed on a C18 column with gradient elution. A tandem mass system coupled with a TurboIonSpray interface operating in simultaneous positive and negative ion multiple reaction monitoring modes was employed for the simultaneous detection of the analytes. Results: The proposed method demonstrated excellent accuracy and repeatability. Conclusion: This method was successfully applied for the pharmacokinetic evaluation of phillyrin and its three metabolites simultaneously.

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