Sensitive Determination of C-Alkylated Flavonoids by HPLC-ESI-MS/MS Using Multiple Reaction Monitoring Approach: Pseudarthria hookeri as a Case Study

2019 ◽  
Vol 57 (10) ◽  
pp. 944-949
Author(s):  
Ina Faraz ◽  
Arslan Ali ◽  
Faraz Ul Haq ◽  
Joseph Tchamgoue ◽  
Simeon F Kouam ◽  
...  
Keyword(s):  
Quality Control ◽  
Method Development ◽  
Control Method ◽  
Raw Materials ◽  
Multiple Reaction Monitoring ◽  
Herbal Medicines ◽  
Reaction Monitoring ◽  
Linear Calibration ◽  
Esi Ms ◽  
Plant Raw Materials

Abstract One of the major problems with the formulation of herbal medicines is the quality control of plant material to ensure its efficacy and safety. Quality control of medicinal plants requires analysis of many bioactive compounds present in the plant. C-alkylated flavonoids are an important bioactive subclass of flavonoids. A simple, rapid, sensitive and selective method is presented here for the quantification of bioactive C-alkylated flavonoids. This is the first quantitative method for analysis of C-alkylated flavonoids based on the multiple reaction monitoring (MRM) approach so far. This study focuses on method development for quantification of bioactive C-alkylated flavonoids. Quantification of a total of five C-alkylated flavonoids was done employing the MRM approach on an HPLC-QqQ-MS instrument. LODs and LOQs for quantified flavonoids were in the range of 0.41–1.32 and 1.23–3.96 ng/mL, respectively. Linear calibration curves between 25 and 1500 ng/mL were obtained with the regression coefficients of ≥0.996. Accuracy (% bias) and precision (% RSD) of the analyses were found to be less than 5%. Developed HPLC-ESI-MS/MS can be employed as a quality control method of plant raw materials.

Cleaning laser spark spectroscopy for online cleaning quality control method development

2010 ◽  
Author(s):  
T. Y. Mutin ◽  
V. N. Smirnov ◽  
V. P. Veiko ◽  
S. A. Volkov
Keyword(s):  
Quality Control ◽  
Method Development ◽  
Control Method ◽  
Quality Control Method ◽  
Laser Spark

scholarly journals Recent Development of Quality Control Methods for Herbal Derived Drug Preparations

2018 ◽  
Vol 13 (12) ◽  
pp. 1934578X1801301 ◽  
Author(s):  
Gunawan Indrayanto
Keyword(s):  
Quality Control ◽  
Raw Materials ◽  
Herbal Medicines ◽  
Complex Nature ◽  
Chemical Marker ◽  
Chemical Profiling ◽  
Chemical Content ◽  
Pharmaceutical Industries ◽  
Microbial Toxins

Pharmaceutical industries should apply rigorous QC (quality control) to ensure the consistency, safety, and efficacy of their herbal derived drug-preparations. QC must be performed at every stage of the production line i.e. incoming raw materials, extractions, in-process control, finished products and keeping samples. Due to the complex nature of the chemical content of herbal drugs, two approaches to QC should be taken, that is quantitative determination of the selected marker(s) compound(s), and metabolite profiling. Contamination of herbal medicines by heavy metals, pesticides, toxic metabolites, microbial toxins, pathogenic microorganisms and other foreign matter should also be evaluated. A combination of chemical profiling and multivariate analysis (MVA) is recommended as the QC tool for the botanical identification method (BIM) of herbs, extracts, herb materials, and herbal drug preparations. Microscopic methods, DNA profiling or chemical marker(s) are not recommended for use as the sole BIM due to the lack of specificity. Only markers that meet certain criteria i.e. quality active (QA) markers can be utilized as a QC tool. The limit specification range of markers used as QC tools should be described in the analytical target profile (ATP). To gain reliable results of any analysis that has been performed at any QC laboratory, the analysis method must be validated according to the newest guidance. Sample detection limit of any toxic compound(s) should be lower than its cut-off value and MPL. The reliability of any results of analysis of a QC laboratory must be evaluated by using QC-samples for each series of measurements.

scholarly journals Application of multiple reaction monitoring for quantitation of sweeteners in food products

2019 ◽  
Vol 10 (3) ◽  
pp. 263-266
Author(s):  
Wafaa Abdou Zaghary ◽  
Ahmed Mostafa Abdalla ◽  
Emily Tawfik Hanna ◽  
Nermeen Abdallah Shoukry
Keyword(s):  
Mass Spectrometry ◽  
Quality Control ◽  
Mobile Phase ◽  
Ammonium Acetate ◽  
Detection Method ◽  
Multiple Reaction Monitoring ◽  
Gradient Flow ◽  
Reaction Monitoring ◽  
Accuracy And Precision ◽  
Ich Guidelines

A coupled UPLC-MS/MS method has been developed and validated for the simultaneous quantitation of food sweeteners stevioside (STV) and aspartame (ASP). Good chromato-graphic separation was achieved on a Hypersil gold 50×2.1 mm (1.9 μm) column, using a gradient flow of 10 mM ammonium acetate, pH = 2.9 adjusted with formic acid, and 10 mM ammonium acetate in acetonitrile:water (95:5, v:v) as the mobile phase. The eluate was introduced to ESI-Mass spectrometer and scanned using multiple reaction monitoring (MRM). The method was robust, reproducible and easy to use and was validated according to ICH guidelines for the accuracy and precision giving acceptable ranges. The utilization of multiple reaction monitoring improved the selectivity of detection. Method was linear in the ranges of 2-250 ng/mL for STV and ASP. Application of this method on laboratory mixtures of the selected sweeteners was successful. The using of mass spectrometry make the method selective and measurement did not affect the presence of impurities, additive and other ingredients of detection due to the simplicity and sensitivity of this method allows the utilization of method in quality control of STV and ASP.

scholarly journals Standardization of Eucalyptus globulus leaves and Cetraria islandica slan

2021 ◽  
pp. 56-63
Author(s):  
Iryna Fito ◽  
Nataliya Stadnytska
Keyword(s):  
Eucalyptus Globulus ◽  
Raw Materials ◽  
Herbal Medicines ◽  
Biologically Active Substances ◽  
Biologically Active ◽  
Qualitative And Quantitative ◽  
Plant Raw Materials ◽  
Further Development ◽  
Layer Chromatography ◽  
Active Substances

The aim. The aim of this study was to propose methods of standardization of Eucalyptus globulus leaves and Cetraria islandica slan by the main group of biologically active substances (BAS). This was done for further development of complex medicines based on Eucalyptus globulus leaves and Cetraria islandica slan. These studies provide an opportunity for further development and standardization of extracts from these plants, and the main aim of this study is the development and standardization of a complex medicine. Materials and methods. Thin layer chromatography was used to determine the presence of these markers. Quantitative values of these biologically active substances were determined by spectrophotometric method according to the relevant methods of the State Pharmacopoeia of Ukraine. Results. Markers for the certification of Cetraria islandica and Eucalyptus globulus have been proposed in this study. Chlorophylls and 1,8-cineole were chosen as a marker for the study of Eucalyptus globulus, and polysaccharides were chosen as a marker for Cetraria islandica. The qualitative and quantitative content of these markers in the studied objects was studied. Further research in this direction will be aimed at developing methods for control and standardization of water and alcohol extracts from these plants. Conclusions. Biologically active substances in medicinal plant raw materials were studied. It is proposed to study extracts and finished products from Eucalyptus globulus leaves and Cetraria islandica slan using these methods. The method of qualitative and quantitative determination of polysaccharides in Cetraria islandica slan is easy to apply and easily reproducible. It is important to be able to standardize Eucalyptus globulus leaves for essential oils and chlorophylls. The biologically active substances-markers found in Eucalyptus globulus leaves and Cetraria islandica slan will be used for the development of herbal medicines, the standardization of which will be carried out for the same compounds.

scholarly journals Quantification of seminolipid by LC-ESI-MS/MS-multiple reaction monitoring: compensatory levels inCgtmice

2010 ◽  
Vol 51 (12) ◽  
pp. 3548-3558 ◽  
Author(s):  
Kessiri Kongmanas ◽  
Hongbin Xu ◽  
Arman Yaghoubian ◽  
Laura Franchini ◽  
Luigi Panza ◽  
...  
Keyword(s):  
Multiple Reaction Monitoring ◽  
Reaction Monitoring ◽  
Esi Ms

scholarly journals NMR Fingerprint Comparison of Cultivated Sideritis spp. from Cyprus

Agronomy ◽  
2021 ◽  
Vol 11 (8) ◽  
pp. 1503
Author(s):  
Ekaterina-Michaela Tomou ◽  
Krystalia Lytra ◽  
Antonios Chrysargyris ◽  
Nikolaos Tzortzakis ◽  
Helen Skaltsa
Keyword(s):  
Positive Impact ◽  
Raw Materials ◽  
Research Work ◽  
Herbal Medicines ◽  
2D Nmr ◽  
Medicinal And Aromatic Plants ◽  
Plant Raw Materials ◽  
Chemical Fingerprints ◽  
Cultivation Practices ◽  
Nmr Methods

Medicinal and Aromatic Plants (MAPs) play an essential role in global health systems, since more than 80% of population use natural products in primary healthcare. Given that the global use of herbal medicines is exponentially increasing, as well as many MAPs products are introduced into the market, many cultivation practices are applied to produce high quality and standardized plant raw materials. Thus, the present study focuses on the chemical fingerprints of two cultivated Sideritis species. In Cyprus, Sideritis cypria Post and S. perfoliata L. subsp. perfoliata are widely used in traditional medicine. To date, there is no research work presenting the comparative chemical profiling between the aforesaid species using NMR methods. In this study, 1D and 2D NMR experiments were used to compare the chemical fingerprints of these species originated from conventional cultivation practices. Iridoids (ajugol, monomelittoside, and melittoside) and one flavone (4′-methyl-isoscutellarein 7-O-[6‴-O-acetyl]-β-D-allosyl(1→2)glucoside) were present in the infusion of S. perfoliata subsp. perfoliata. The phenylethanoid glycoside, acteoside, was detected in both samples. The phytochemical profiles of these cultivated species were similar to those of Sideritis species, indicating the positive impact of cultivation practices in MAPs.

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