scholarly journals Ion Mobility Spectrometry-Hydrogen Deuterium Exchange Mass Spectrometry of Anions: Part 1. Peptides to Proteins

2014 ◽  
Vol 26 (4) ◽  
pp. 564-576 ◽  
Author(s):  
Gregory C. Donohoe ◽  
Mahdiar Khakinejad ◽  
Stephen J. Valentine
Keyword(s):  
Mass Spectrometry ◽  
Ion Mobility Spectrometry ◽  
Ion Mobility ◽  
Deuterium Exchange ◽  
Hydrogen Deuterium Exchange ◽  
Exchange Mass Spectrometry ◽  
Hydrogen Deuterium ◽  
Deuterium Exchange Mass Spectrometry

scholarly journals Distinguishing Between Isobaric Ions Using Microdroplet Hydrogen-Deuterium Exchange Mass Spectrometry

2021 ◽  
Author(s):  
Xiaowei Song ◽  
Jia Li ◽  
Mohammad Mofidfar ◽  
Richard N. Zare
Keyword(s):  
Mass Spectrometry ◽  
Ion Mobility ◽  
Deuterium Exchange ◽  
Charge Ratio ◽  
Hydrogen Deuterium Exchange ◽  
Exchange Mass Spectrometry ◽  
Different Types ◽  
Hydrogen Deuterium ◽  
Deuterium Exchange Mass Spectrometry ◽  
Droplet Spray

Isobaric ions having the same mass-to-charge ratio cannot be separately identified by mass spectrometry (MS) alone, but this limitation can be overcome using hydrogen-deuterium exchange (HDX) in microdroplets. Because isobaric ions may contain a varied number of exchangeable sites and different types of functional groups, each one produces a unique MS spectral pattern after droplet spray HDX without the need for MS/MS experiments or introduction of ion mobility measurements. As an example of the power of this approach, isobaric ions in urinary metabolic profiles are identified and used to distinguish between healthy individuals and those having bladder cancer.

scholarly journals Distinguishing between Isobaric Ions Using Microdroplet Hydrogen–Deuterium Exchange Mass Spectrometry

Metabolites ◽  
2021 ◽  
Vol 11 (11) ◽  
pp. 728
Author(s):  
Xiaowei Song ◽  
Jia Li ◽  
Mohammad Mofidfar ◽  
Richard N. Zare
Keyword(s):  
Mass Spectrometry ◽  
Ion Mobility ◽  
Deuterium Exchange ◽  
Charge Ratio ◽  
Hydrogen Deuterium Exchange ◽  
Exchange Mass Spectrometry ◽  
Different Types ◽  
Hydrogen Deuterium ◽  
Deuterium Exchange Mass Spectrometry ◽  
Droplet Spray

Isobaric ions having the same mass-to-charge ratio cannot be separately identified by mass spectrometry (MS) alone, but this limitation can be overcome by using hydrogen–deuterium exchange (HDX) in microdroplets. Because isobaric ions may contain a varied number of exchangeable sites and different types of functional groups, each one produces a unique MS spectral pattern after droplet spray HDX without the need for MS/MS experiments or introduction of ion mobility measurements. As an example of the power of this approach, isobaric ions in urinary metabolic profiles are identified and used to distinguish between healthy individuals and those having bladder cancer.

Sign in / Sign up

Export Citation Format

Share Document