β-Glucosidase production by Trichoderma reesei and Thermoascus aurantiacus by solid state cultivation and application of enzymatic cocktail for saccharification of sugarcane bagasse

Aims: The aim of this research was to evaluate the SSC of Aspergillus niger and Trichoderma reesei alone and in consortium for the citric acid production from sugarcane bagasse. Methods: Solid-State Cultivation (SSC) is characterized by microbial growth on solid supports often agroindustrial by-products in the near absence of free water conditions. Citric acid, an important by-product used in various sectors of the economy, can be obtained from the SSC using sugarcane bagasse, minimizing the cost of its production with the use of this industrial by-product. Results: The fungal consortiums have advantages over the isolated cultures, highlighting the best use of substrates due to enzyme supplementation, especially hydrolases. Conclusion: The results indicated a specific production of citric acid of 2.51 mg per g of biomass per hour, which was higher than that obtained when using the two fungi alone.

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Comparative secretome analysis of Trichoderma asperellum S4F8 and Trichoderma reesei Rut C30 during solid-state fermentation on sugarcane bagasse

Biotechnology for Biofuels ◽

10.1186/1754-6834-6-172 ◽

2013 ◽

Vol 6 (1) ◽

pp. 172 ◽

Cited By ~ 46

Author(s):

Isa Marx ◽

Niël van Wyk ◽

Salome Smit ◽

Daniel Jacobson ◽

Marinda Viljoen-Bloom ◽

...

Keyword(s):

Solid State ◽

Trichoderma Reesei ◽

Sugarcane Bagasse ◽

Solid State Fermentation ◽

Trichoderma Asperellum ◽

Secretome Analysis ◽

Trichoderma Reesei Rut C30 ◽

Rut C30

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Solid-state cultivation of Aspergillus niger–Trichoderma reesei from sugarcane bagasse with vinasse in bench packed-bed column bioreactor

10.21203/rs.3.rs-158166/v1 ◽

2021 ◽

Author(s):

Laura Macedo Rocha ◽

Beatriz Silva Campanhol ◽

Reinaldo Gaspar Bastos

Keyword(s):

Citric Acid ◽

Solid State ◽

Aspergillus Niger ◽

Trichoderma Reesei ◽

Sugarcane Bagasse ◽

Maximum Yield ◽

Hydrolytic Enzymes ◽

Packed Bed ◽

Industrial Applications ◽

Acid Yield

Abstract Solid-state cultivation (SSC) is microbial growth on solid supports under limited water conditions. Citric acid, one of the products obtained by SSC, is a microbial aerobic metabolic product with various industrial applications. Several wastes from agro-industries are used in SSC, such as sugarcane bagasse and vinasse. As xylanolytic enzymes of inoculum breakdown the lignocellulosic material (bagasse), mixed fungal cultures or co-cultures are used in these SSC. Thus, this study aims to evaluate the effect of inoculum (Aspergillus niger and Trichoderma reesei consortium) in the production of citric acid from sugarcane bagasse impregnated with vinasse using bench packed bed reactors (PBR). The results show the importance of T. reesei in inoculum with A. niger at a ratio of 50:50 and 25:75, suggesting the use of solid support due to the complementation of the hydrolytic enzymes. The highest concentration of approximately 1000 mg L− 1 of citric acid yield for 100 mm of bed height in 48 and 72 h, with the maximum yield from glucose to citric acid (2.2 mg citric acid mg glucose−1). kLa indicates that maintaining solid moisture and liquid film thickness is important to keep the oxygen transfer in SSC.

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