scholarly journals Solid-state cultivation of Aspergillus niger–Trichoderma reesei from sugarcane bagasse with vinasse in bench packed-bed column bioreactor

2021 ◽  
Author(s):  
Laura Macedo Rocha ◽  
Beatriz Silva Campanhol ◽  
Reinaldo Gaspar Bastos
Keyword(s):  
Citric Acid ◽  
Solid State ◽  
Aspergillus Niger ◽  
Trichoderma Reesei ◽  
Sugarcane Bagasse ◽  
Maximum Yield ◽  
Hydrolytic Enzymes ◽  
Packed Bed ◽  
Industrial Applications ◽  
Acid Yield

Abstract Solid-state cultivation (SSC) is microbial growth on solid supports under limited water conditions. Citric acid, one of the products obtained by SSC, is a microbial aerobic metabolic product with various industrial applications. Several wastes from agro-industries are used in SSC, such as sugarcane bagasse and vinasse. As xylanolytic enzymes of inoculum breakdown the lignocellulosic material (bagasse), mixed fungal cultures or co-cultures are used in these SSC. Thus, this study aims to evaluate the effect of inoculum (Aspergillus niger and Trichoderma reesei consortium) in the production of citric acid from sugarcane bagasse impregnated with vinasse using bench packed bed reactors (PBR). The results show the importance of T. reesei in inoculum with A. niger at a ratio of 50:50 and 25:75, suggesting the use of solid support due to the complementation of the hydrolytic enzymes. The highest concentration of approximately 1000 mg L− 1 of citric acid yield for 100 mm of bed height in 48 and 72 h, with the maximum yield from glucose to citric acid (2.2 mg citric acid mg glucose−1). kLa indicates that maintaining solid moisture and liquid film thickness is important to keep the oxygen transfer in SSC.

scholarly journals Citric Acid Production by the Solid-State Cultivation Consortium of Aspergillus Niger and Trichoderma Reesei from Sugarcane Bagasse

2020 ◽  
Vol 14 (1) ◽  
pp. 32-41
Author(s):  
Reinaldo G. Bastos ◽  
Hiléia C. Ribeiro
Keyword(s):  
Citric Acid ◽  
Solid State ◽  
Aspergillus Niger ◽  
Trichoderma Reesei ◽  
Sugarcane Bagasse ◽  
Acid Production ◽  
Free Water ◽  
Citric Acid Production ◽  
Solid Supports ◽  
The Cost

Aims: The aim of this research was to evaluate the SSC of Aspergillus niger and Trichoderma reesei alone and in consortium for the citric acid production from sugarcane bagasse. Methods: Solid-State Cultivation (SSC) is characterized by microbial growth on solid supports often agroindustrial by-products in the near absence of free water conditions. Citric acid, an important by-product used in various sectors of the economy, can be obtained from the SSC using sugarcane bagasse, minimizing the cost of its production with the use of this industrial by-product. Results: The fungal consortiums have advantages over the isolated cultures, highlighting the best use of substrates due to enzyme supplementation, especially hydrolases. Conclusion: The results indicated a specific production of citric acid of 2.51 mg per g of biomass per hour, which was higher than that obtained when using the two fungi alone.

scholarly journals Citric Acid Production by Aspergillus niger through Solid-State Fermentation on Sugarcane Bagasse

1970 ◽  
Vol 25 (1) ◽  
pp. 9-12 ◽  
Author(s):  
Abdullah Al-Mahin ◽  
Shek Mahdi Hasan ◽  
Mahboob Hossain Khan ◽  
Rehana Begum
Keyword(s):  
Citric Acid ◽  
Solid State ◽  
Aspergillus Niger ◽  
Sugarcane Bagasse ◽  
Solid State Fermentation ◽  
Acid Production ◽  
Sucrose Solution ◽  
Citric Acid Production ◽  
Sugar Utilization ◽  
Ig G

Four isolates of Aspergillus niger (viz., CA16, 14/20, HB3 and 318) were used for citric acid production using sugarcane bagasse as a substrate and sucrose solution as a moistening agent. Using 3 g of sugarcane bagasse as substrate, the highest citric acid production was obtained when 10 ml of 14% sucrose solution was used as moistening agent. Maximum citric acid production was found after 11 days fermentation for all isolates of A. niger. Both sugar utilization as well as citric acid production was found highest in the presence of Prescott salt by A. niger 318 (196.21 ìg/g), followed next by A. niger 14/20 (103.06 ìg/g). However, in absence of Prescott salt both sugar utilization and citric acid production was highest (50.01 ìg/g) by A. niger 14/20. In general, extension of the fermentation (up to 11 days) resulted in an increase in citric acid, total titratable acid and biomass, and decrease in both residual sugar concentrations. Keywords: Citric acid; Solid-state fermentation; Aspergillus niger; Sugarcane bagasseDOI: http://dx.doi.org/10.3329/bjm.v25i1.4664 Bangladesh J Microbiol, Volume 25, Number 1, June 2008, pp 9-12

scholarly journals Valorization of Banana Peel for Citric Acid Production under Solid State Fermentation with Aspergillus niger

2020 ◽  
Vol 34 (1) ◽  
pp. 49-57
Author(s):  
Teerin Chysirichote
Keyword(s):  
Citric Acid ◽  
Solid State ◽  
Aspergillus Niger ◽  
Solid State Fermentation ◽  
Packed Bed ◽  
Aeration Rate ◽  
Banana Peel ◽  
Initial Ph ◽  
Glass Column ◽  
Cooling Air

Valorization of banana peel (BP) through solid state fermentation (SSF) was aimed<br /> in this research. The appropriate conditions of citric acid (CA) production by SSF of<br /> Aspergillus niger were investigated. Firstly, the optimum initial pH of the BP and the<br /> aeration rate were studied by conducting SSF in a 250-mL flask and 2-L glass column,<br /> respectively. It was found that the initial pH of the BP and aeration rates affected the CA<br /> production. The results showed that the initial pH of 5.0 and 1.0 vvm aeration were appropriate for the CA production of A. niger using BP as a substrate. The problem of rising temperature during SSF was found when applying the optimum condition to the SSF<br /> in the 20-L packed bed bioreactor (PBB), which caused a decrease in the CA production<br /> compared to that of the glass column. The cooling air jacket constructed to the PBB to<br /> remove the heat during the SSF helped increase the CA production from that in the PBB.<br /> The maximum CA production in the 20-L air-jacketed PBB was 124.0±19.2 mg g–1DS.

scholarly journals Comparative Studies on Production of Cell Wall-Degrading Hydrolases by Trichoderma reesei and T. viride in Submerged and Solid-State Cultivations

1970 ◽  
Vol 23 (2) ◽  
pp. 149-155 ◽  
Author(s):  
Isidore Gomes ◽  
Mohammad Shaheen ◽  
Sabita Rezwana Rahman ◽  
Donald James Gomes
Keyword(s):  
Solid State ◽  
Trichoderma Reesei ◽  
Soluble Sugars ◽  
Trichoderma Viride ◽  
Hydrolytic Enzymes ◽  
Tween 80 ◽  
Industrial Applications ◽  
Ionic Surfactant ◽  
Submerged Cultures ◽  
Culture Methods

Lignocellulose-degrading organisms have been used for the conversion of lignocellulosic materials into soluble sugars or solvents in several biotechnological and industrial applications. Complete degradation of complex highly structured lignocellosics requires a concerted action of a wide array hydrolytic enzymes. In this study, two newly isolated fungi, Trichoderma reesei and T. viride, were examined for their ability to produce cellulolytic, xylanolytic and pectinolytic enzymes in submerged and solid-state fermentations. The fungi produced appreciable amounts of the enzymes when grown on potentially low cost lignocellulosic agricultural residues like wheat bran, sugar cane bagasse and corncobs. T. viride seems to be superior to T. reesei with respect to enzyme productions both in submerged and solid-state cultivations. There was a distinct influence of culture methods on the production of the enzymes by the fungi. The enzyme productions were higher in solid-state fermentations than in submerged fermentations. However, taking into consideration of enzyme yields per gram substrate, it was found that the yields were many-fold higher in submerged cultures than in solid-state fermentations. The recovery of the enzymes from fermented slurries in solidstate fermentations was enhanced by using non-ionic surfactant Tween 80 as leaching agent. The enzymes produced by the fungi displayed optimum activities at pH range between 4.5 and 5.5, and at temperatures between 50 and 55°C. The fungi merit further attention as potential sources of industrial enzymes, as they exhibited some excellent properties including the ability to synthesize a wide array of hydrolytic enzymes while grown on cheap and readily available lignocellulosic residues. Keywords: Lignocellulosics, cellulase, xylanase, pectinase, Trichoderma reesei, Trichoderma viride, submerged culture, solid-state fermentationDOI: http://dx.doi.org/10.3329/bjm.v23i2.882 Bangladesh J Microbiol, Volume 23, Number 2, December 2006, pp 149-155

scholarly journals Research on fermentation process of citric acid in solid state using sugarcane bagasse and Aspergillus Niger

2014 ◽  
Vol 17 (1) ◽  
pp. 94-101
Author(s):  
Hanh Thi Minh Truong ◽  
Tuan Anh Nguyen
Keyword(s):  
Citric Acid ◽  
Solid State ◽  
Aspergillus Niger ◽  
Sugarcane Bagasse ◽  
Food Industry ◽  
Fermentation Process ◽  
Fixed Amount ◽  
Fermentation Time ◽  
Content Change ◽  
Wide Range

Citric acid is an organic acid that has a wide range of applications in food industry and other industries. In the pharmaceutical industry, citric acid is used to produce different metal citrate salts so that these minerals can be biologically used in many medicines. For example, iron citrate made from citric acid provides iron to protect human blood, or is used to produce medicines in tablet or cream forms and cosmetics. In this research, we investigated effects of technological factors on the fermentation process of citric acid in the solid state using sugarcane bagasse and Aspergillus Niger. We conducted an experiment to determine the content of citric acid obtained in fermented liquid using HPLC. The result showed that substrate humidity, culture ratio and duration significantly influence on the fermentation process. Through the investigation of water content change in solid state from sugarcane bagasse, we indicated that during the fermentation time, a fixed amount of water to be supplemented every day to maintain the initial humidity. Based on the results, we recommend a fermentation process of citric acid using sugarcane bagasse under the following condition: substrate humidity 80%, temperature 30OC, 9-day duration, A.niger ratio 10% (v/w). The content of citric acid that can be obtained under the above condition is 12.02g/100g of sugarcane bagasse.

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