Agrobacterium-mediated In-planta transformation of bread wheat (Triticum aestivum L.)

2021 ◽  
Author(s):  
Priyanka Singh ◽  
Kanika Kumar
Keyword(s):  
Triticum Aestivum ◽  
Bread Wheat ◽  
Triticum Aestivum L ◽  
In Planta Transformation ◽  
In Planta

Development of simple and efficient in Planta transformation method for wheat (Triticum aestivum L.) using Agrobacterium tumefaciens

2006 ◽  
Vol 102 (3) ◽  
pp. 162-170 ◽  
Author(s):  
Putu Supartana ◽  
Tsutomu Shimizu ◽  
Masahiro Nogawa ◽  
Hidenari Shioiri ◽  
Tadashi Nakajima ◽  
...  
Keyword(s):  
Triticum Aestivum ◽  
Agrobacterium Tumefaciens ◽  
Triticum Aestivum L ◽  
Transformation Method ◽  
In Planta Transformation ◽  
In Planta

scholarly journals Genetic transformation of promising genotypes of winter bread wheat by in planta method

2021 ◽  
Vol 28 ◽  
pp. 106-111
Author(s):  
L. V. Slivka ◽  
O. V. Dubrovna
Keyword(s):  
Triticum Aestivum ◽  
Transgenic Plants ◽  
Genetic Transformation ◽  
Bread Wheat ◽  
Optical Density ◽  
Triticum Aestivum L ◽  
In Planta ◽  
Agrobacterium Mediated Transformation ◽  
Winter Bread Wheat ◽  
Inoculation Medium

Aim. Optimization of conditions and genetic transformation of new promising genotypes of winter bread wheat (Triticum aestivum L.) by in planta method. Methods. Agrobacterium-mediated transformation by the in planta method using strain AGL0 and vector construct pBi-OAT. Results. The influence of air temperature, optical density of cells of agrobacterial suspension, inoculation day and composition of inoculation medium on the frequency of obtaining transgenic plants of new promising genotypes of winter wheat was studied. The dependence of the frequency of obtaining transgenic plants on environmental conditions, in particular temperature, has been established. It was found that the temperature regime of 20-22 °C provided the largest number (4.4%) of wheat transformants, and when the temperature is reduced to 16-18 °C there is a decrease in the efficiency of T-DNA transfer into the plant genome and the lowest frequency of transformation is observed. Conclusions. The largest number of transformants was obtained using a inoculation medium without sucrose, the optical density of cells of the agrobacterial suspension of 0.4 op.od. and inoculation on the third day after castration of ears. Keywords: Triticum aestivum L., Agrobacterium-mediated transformation, ornithine-δ-aminotransferase gene.

Effect of Cold Pretreatment and Incubation Temperature on Bread Wheat (Triticum aestivum L.) Anther Culture

2001 ◽  
Vol 29 (3-4) ◽  
pp. 331-338 ◽  
Author(s):  
I. N. Xynias ◽  
I. A. Zamani ◽  
E. Gouli-Vavdinoudi ◽  
D. G. Roupakias
Keyword(s):  
Triticum Aestivum ◽  
Anther Culture ◽  
Bread Wheat ◽  
Incubation Temperature ◽  
Triticum Aestivum L ◽  
Cold Pretreatment

scholarly journals QTL mapping of seedling biomass and root traits under different nitrogen conditions in bread wheat (Triticum aestivum L.)

2021 ◽  
Vol 20 (5) ◽  
pp. 1180-1192
Author(s):  
Meng-jiao YANG ◽  
Cai-rong WANG ◽  
Muhammad Adeel HASSAN ◽  
Yu-ying WU ◽  
Xian-chun XIA ◽  
...  
Keyword(s):  
Triticum Aestivum ◽  
Qtl Mapping ◽  
Bread Wheat ◽  
Root Traits ◽  
Triticum Aestivum L ◽  
Seedling Biomass ◽  
Nitrogen Conditions

Genetic relationships between resistances to Fusarium head blight and crown rot in bread wheat (Triticum aestivum L.)

2010 ◽  
Vol 121 (5) ◽  
pp. 941-950 ◽  
Author(s):  
Hao Bing Li ◽  
Guo Qiang Xie ◽  
Jun Ma ◽  
Gui Ru Liu ◽  
Shu Min Wen ◽  
...  
Keyword(s):  
Triticum Aestivum ◽  
Fusarium Head Blight ◽  
Bread Wheat ◽  
Genetic Relationships ◽  
Triticum Aestivum L ◽  
Crown Rot ◽  
Head Blight

scholarly journals Genetic and in silico comparative mapping of the polyphenol oxidase gene in bread wheat (Triticum aestivum L.)

2005 ◽  
Vol 6 (1) ◽  
pp. 81-81
Author(s):  
Rosy Raman ◽  
Harsh Raman ◽  
Katie Johnstone ◽  
Chris Lisle ◽  
Alison Smith ◽  
...  
Keyword(s):  
Triticum Aestivum ◽  
Bread Wheat ◽  
Polyphenol Oxidase ◽  
Comparative Mapping ◽  
In Silico ◽  
Triticum Aestivum L ◽  
Oxidase Gene
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