Production of colored foliage phenotypes in Kalanchoe blossfeldiana by ectopic expression of R2R3 MYB genes

Abstract Abstract Background: Anthocyanins contribute to coloration and antioxidation effects in different plant tissues. MYB transcription factors have been demonstrated to be a key regulator for anthocyanin synthesis in many plants. However, little information was available about the MYB genes in the halophyte species Eutrema salsugineum . Result: Here we report the identification of an important anthocyanin biosynthesis regulator Es MYB90 from Eutrema salsugineum , which is a halophyte tolerant to multiple abiotic stresses. Our phylogenetic and localization analyses supported that Es MYB90 is an R2R3 type of MYB transcription factor. Ectopic expression of EsMYB90 in tobacco and Arabidopsis enhanced pigmentation and anthocyanin accumulation in various organs. The transcriptome analysis revealed that 42 genes upregulated by Es MYB90 in 35S : EsMYB90 tobacco transgenic plants are required for anthocyanin biosynthesis. Moreover, our qRT-PCR results showed that Es MYB90 promoted expression of early ( PAL , CHS , and CHI ) and late ( DFR , ANS , and UFGT ) anthocyanin biosynthesis genes in stems, leaves, and flowers of 35S : EsMYB90 tobacco transgenic plants. Conclusions: Our results indicated that Es MYB90 is a MYB transcription factor, which regulates anthocyanin biosynthesis genes to control anthocyanin biosynthesis. Our work provides a new tool to enhance anthocyanin production in various plants. Keywords : Anthocyanin, flavonoid, Eutrema salsugineum , R2R3 MYB transcription factor, Es MYB90, transcriptional regulation, anthocyanin biosynthesis genes.

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Ectopic expression of the Anthurium andraeanum (Hort.) R2R3-MYB genes AaMYB4 and AaMYB5 enhance the flower color in transgenic tobacco

Plant Cell Tissue and Organ Culture (PCTOC) ◽

10.1007/s11240-019-01667-7 ◽

2019 ◽

Vol 139 (1) ◽

pp. 105-117

Author(s):

Chonghui Li ◽

Jian Qiu ◽

Guangsui Yang ◽

Surong Huang ◽

Junmei Yin

Keyword(s):

Transgenic Tobacco ◽

Ectopic Expression ◽

Flower Color ◽

Anthurium Andraeanum ◽

Myb Genes ◽

R2r3 Myb

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Identification of the Eutrema Salsugineum EsMYB90 gene important for anthocyanin biosynthesis

10.21203/rs.2.18301/v3 ◽

2020 ◽

Author(s):

Yuting Qi ◽

Caihong Gu ◽

Xingjun Wang ◽

Shiqing Gao ◽

Changsheng Li ◽

...

Keyword(s):

Transcription Factor ◽

Transgenic Plants ◽

Anthocyanin Biosynthesis ◽

Ectopic Expression ◽

Myb Transcription Factor ◽

Anthocyanin Synthesis ◽

Eutrema Salsugineum ◽

Tobacco Transgenic Plants ◽

Myb Genes ◽

R2r3 Myb

Abstract Background: Anthocyanins contribute to coloration and antioxidation effects in different plant tissues. MYB transcription factors have been demonstrated to be a key regulator for anthocyanin synthesis in many plants. However, little information was available about the MYB genes in the halophyte species Eutrema salsugineum . Result: Here we report the identification of an important anthocyanin biosynthesis regulator Es MYB90 from Eutrema salsugineum , which is a halophyte tolerant to multiple abiotic stresses. Our phylogenetic and localization analyses supported that Es MYB90 is an R2R3 type of MYB transcription factor. Ectopic expression of EsMYB90 in tobacco and Arabidopsis enhanced pigmentation and anthocyanin accumulation in various organs. The transcriptome analysis revealed that 42 genes upregulated by Es MYB90 in 35S : EsMYB90 tobacco transgenic plants are required for anthocyanin biosynthesis. Moreover, our qRT-PCR results showed that Es MYB90 promoted expression of early ( PAL , CHS , and CHI ) and late ( DFR , ANS , and UFGT ) anthocyanin biosynthesis genes in stems, leaves, and flowers of 35S : EsMYB90 tobacco transgenic plants. Conclusions: Our results indicated that Es MYB90 is a MYB transcription factor, which regulates anthocyanin biosynthesis genes to control anthocyanin biosynthesis. Our work provides a new tool to enhance anthocyanin production in various plants. Keywords : Anthocyanin, flavonoid, Eutrema salsugineum , R2R3 MYB transcription factor, Es MYB90, transcriptional regulation, anthocyanin biosynthesis genes.

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First Report of Powdery Mildew of Kalanchoe blossfeldiana caused by Sphaerotheca fuliginea (Podosphaera fuliginea) in the Pacific Northwest

Plant Health Progress ◽

10.1094/php-2003-0417-02-hn ◽

2003 ◽

Vol 4 (1) ◽

pp. 39

Author(s):

Dean A. Glawe ◽

Rita Hummel ◽

Grace Jack

Keyword(s):

Powdery Mildew ◽

Pacific Northwest ◽

Sphaerotheca Fuliginea ◽

First Report ◽

Major Disease ◽

The Pacific ◽

Kalanchoe Blossfeldiana ◽

Extension Center ◽

Kalanchoë Blossfeldiana

Kalanchoe blossfeldiana Poelln. is a common ornamental houseplant. Although powdery mildew is a major disease of this species, there are no published reports of it in the Pacific Northwest. In August, 2002, powdery mildew was observed on six indoor K. blossfeldiana plants in an office and adjacent laboratory at the Puyallup Research and Extension Center. Accepted for publication 25 March 2003. Published 17 April 2003.

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Antiplasmid Potential of Kalanchoe blossfeldiana Against Multidrug Resistance Pseudomonas aeruginosa

British Journal of Pharmacology and Toxicology ◽

10.19026/bjpt.5.5184 ◽

2014 ◽

Vol 5 (6) ◽

pp. 177-185

Author(s):

ZirakFaqe Ahmed Abdulrahman

Keyword(s):

Pseudomonas Aeruginosa ◽

Multidrug Resistance ◽

Kalanchoe Blossfeldiana ◽

Kalanchoë Blossfeldiana

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Genome-wide identification, functional prediction, and evolutionary analysis of the R2R3-MYB superfamily in Brassica napus

Genome ◽

10.1139/gen-2017-0059 ◽

2017 ◽

Vol 60 (10) ◽

pp. 797-814 ◽

Cited By ~ 23

Author(s):

Ali Hajiebrahimi ◽

Hajar Owji ◽

Shiva Hemmati

Keyword(s):

Brassica Napus ◽

Purifying Selection ◽

Gene Clusters ◽

Regulatory Elements ◽

Post Inoculation ◽

Evolutionary Analysis ◽

Salinity Treatment ◽

Myb Genes ◽

R2r3 Myb ◽

Leptosphaeria Biglobosa

R2R3-MYB transcription factors (TFs) have been shown to play important roles in plants, including in development and in various stress conditions. Phylogenetic analysis showed the presence of 249 R2R3-MYB TFs in Brassica napus, called BnaR2R3-MYB TFs, clustered into 38 clades. BnaR2R3-MYB TFs were distributed on 19 chromosomes of B. napus. Sixteen gene clusters were identified. BnaR2R3-MYB TFs were characterized by motif prediction, gene structure analysis, and gene ontology. Evolutionary analysis revealed that BnaR2R3-MYB TFs are mainly formed as a result of whole-genome duplication. Orthologs and paralogs of BnaR2R3-MYB TFs were identified in B. napus, B. rapa, B. oleracea, and Arabidopsis thaliana using synteny-based methods. Purifying selection was pervasive within R2R3-MYB TFs. Kn/Ks values lower than 0.3 indicated that BnaR2R3-MYB TFs are being functionally converged. The role of gene conversion in the formation of BnaR2R3-MYB TFs was significant. Cis-regulatory elements in the upstream regions of BnaR2R3-MYB genes, miRNA targeting BnaR2R3MYB TFs, and post translational modifications were identified. Digital expression data revealed that BnaR2R3-MYB genes were highly expressed in the roots and under high salinity treatment after 24 h. BnaMYB21, BnaMYB141, and BnaMYB148 have been suggested for improving salt-tolerant B. napus. BnaR2R3-MYB genes were mostly up regulated on the 14th day post inoculation with Leptosphaeria biglobosa and L. maculan. BnaMYB150 is a candidate for increased tolerance to Leptospheria in B. napus.

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Uso del fertilizante (15-20-25) en la etapa de floración de Kalanchoe blossfeldiana

Revista Biológico Agropecuaria Tuxpan ◽

10.47808/revistabioagro.v5i2.124 ◽

2017 ◽

Vol 5 (2) ◽

pp. 114-118

Author(s):

Ana Cristina Noriega Lucio ◽

Darío López Castro ◽

Rocío Rodríguez Cabrera ◽

Sergio Omar Nieto Rosaliano ◽

Pablo Elorza Martínez

Keyword(s):

Kalanchoe Blossfeldiana ◽

El Alto ◽

Kalanchoë Blossfeldiana

Se evaluaron 4 dosis del fertilizante 15-20-25 fueron 2, 3, 4 y 5 gramos por litro de agua más un testigo. El objetivo fue Determinar la mejor dosis de 15-20-25 para favorecer la floración de Kalanchoe blossfeldiana. Los resultados mostraron que la variable Contenido de clorofila a los 90 días el mejor tratamiento fue el No. 4, con54.95 unidades SPAD, con una diferencia significativa del tratamiento 5 que fue el testigo con 44.64. En la variable de Diámetro de tallo el tratamiento que tuvo mejor resultado fue el tratamiento 2 con 0.741 centímetro, teniendo una diferencia significativa con el testigo. En la variable de número de hojas, el Tratamiento que tuvo mayor número fue el 4 con un total de 20 hojas. En este sentido vemos que el tratamiento que funcionó de mejor manera y que obtuvo mayor contenido de clorofila, mayor diámetro de tallo y numero de hojas fue el tratamiento No. 4, con una dosis de 5 gramos por litro, donde se pudo observar plantas vigorosas de hojas gruesas y color verde obscuro por el alto contenido de nitrógeno registrado en la planta siendo además las que primero iniciaron la etapa de floración y mejor calidad de ramo floral.

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