Segregation ratio in selfed and crossed progenies demonstrates single dominant gene inheritance of day-neutrality in strawberry

Faba bean (Vicia faba L.) is one of the oldest grain legumes and is grown in many countries for both human consumption and animal feed. Faba bean rust, caused by Uromyces viciae-fabae, is a serious disease of faba beans in the subtropical agricultural region of Australia. Experiments were conducted to assess the genetic variation for rust resistance in Australian faba bean germplasm and to determine the genetic basis of rust resistance in selected germplasm. Resistant lines were identified, subsequently crossed to agronomically suitable parents and the ensuing progeny were evaluated for resistance. Many derived lines showed a higher level of resistance than the current cultivars, although none were rated immune. This level of resistance was considered adequate for reliable crop production when combined with limited fungicide application. Genetic studies from the seedling test of F2 and F3 progenies derived from two crosses based on two different sources of resistance showed three distinct responses; highly resistant, moderately resistant and susceptible. However, no homozygous family with a moderate response was found in the F3 progeny test, hence, this infection type could not be attributed to independent gene(s). The segregation ratio in both F2 and F3 in the population derived from Doza#12035, a selection from the commercial cultivar Doza, indicated a single dominant gene was responsible for conferring resistance. In the other population developed from the resistant parent Ac1655, it is likely that also a single dominant gene confers resistance even though the F3 segregation ratio deviated significantly from a one gene hypothesis. An allelism test revealed that each of the resistant parents (Doza#12035 and Ac1655) carried a single and independent gene for resistance, thus providing at least two genes for breeders to choose or pyramid for improving the rust resistance in faba bean.

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Inheritance of stem solidness in eight durum wheat crosses

Canadian Journal of Plant Science ◽

10.4141/p01-053 ◽

2002 ◽

Vol 82 (4) ◽

pp. 661-664 ◽

Cited By ~ 16

Author(s):

F. R. Clarke ◽

J. M. Clarke ◽

R. E. Knox

Keyword(s):

Yield Loss ◽

Triticum Turgidum ◽

Doubled Haploids ◽

Dominant Gene ◽

Segregation Ratio ◽

Field Trials ◽

Single Dominant Gene ◽

Gene Model ◽

Segregation Ratios ◽

Stem Solidness

Yield loss from sawfly (Cephus cinctus Norton) can be prevented by growing solid-stemmed wheat. No solid-stemmed durum cultivars (Triticum turgidumL. var. durum) are registered in Canada, even though solid-stemmed lines are available. Understanding the inheritance of stem solidness in such lines would be useful to breeders. Eight crosses were made between solid-stemmed and hollow-stemmed durums: Trinakria/DT369, Trinakria/DT367, Hugenot G/DT369, Hugenot W/DT369, 8678-1048A/Hugenot W, 8678-1048A/Hugen ot G, Kamilaroi/Hugenot G, and W9262-260D3/Kofa. Trinakria, Hugenot G, Hugenot W and W9262-260D3 are solid-stemmed and the other parents are hollow stemmed. Stem solidness was measured in field trials. The F1 were solid-stemmed, and the F2 of five of the seven crosses were not significantly different (P > 0.05) from a three solid-stemmed to one hollow-stemmed segregation ratio. The backcross of the solid-stemmed parents with the F1 was 100% solid-stemmed, and the backcross of the hollow-stemmed parents with the F1 was not significantly different (P > 0.05) from an expected one solid-stemmed to one hollow-stemmed segregation ratio. The F1 derived doubled haploids did not differ significantly (P > 0.05) from an expected one solid-stemmed to one hollow-stemmed segregation ratio. The expression of stem solidness fit the expected segregation ratios for a single dominant gene model in most of the crosses, which suggests that stem solidness in the four durums evaluated is controlled by a single dominant gene. Key words: Inheritance, sawfly, solid-stemmed, Triticum turgidum L. var. durum

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Powdery mildew resistance in soybean PI 243540 is controlled by a single dominant gene

Canadian Journal of Plant Science ◽

10.4141/cjps09070 ◽

2010 ◽

Vol 90 (6) ◽

pp. 939-942 ◽

Cited By ~ 1

Author(s):

S.-T. Kang ◽

M.A. Rouf Mian

Keyword(s):

Powdery Mildew ◽

Powdery Mildew Resistance ◽

Dominant Gene ◽

Genetic Resistance ◽

Segregation Ratio ◽

Common Disease ◽

Growth Stages ◽

Single Dominant Gene ◽

Sources Of Resistance ◽

Mildew Resistance

Powdery mildew (Microsphaera diffusa Cooke & Peck) is a common disease of soybean in many countries of the world, including the northern United States of America and parts of Canada. The genetic resistance of soybean to M. diffusa is known to be controlled by a single locus with three alleles designated as Rmd, Rmd-c and rmd. Identification and characterization of sources of resistance is a prerequisite for the development of resistant cultivars. The objective of this study was to determine the inheritance of powdery mildew resistance in a plant introduction (PI) from Japan, PI 243540. The inheritance of powdery mildew was determined in a segregating population from a cross between powdery mildew susceptible Ohio cultivar Wyandot and PI 243540. The parents and the progeny showed a consistent response to powdery mildew for all growth stages of plants. The two parents, the F1, F2, and F2:3 families from the cross were screened in a greenhouse and field following inoculation with M. diffusa. All F1 plants were resistant to M. diffusa and χ2 analysis for segregation in the population of 343 F2 plants indicated a tight fit for a 3:1 (P = 0.78) ratio, indicative of a single dominant gene. In the next generation, the 334 F2-derived families fit an expected 1 resistant:2 segregating:1 susceptible segregation ratio (P =0.88), which confirmed the results obtained in the F2 generation. Our results indicate that the powdery mildew resistance derived from PI 243540 is controlled by a single dominant gene linked to the Rmd/ Rmd-c/rmd locus. The simple inheritance of this gene should make it relatively easy to find linked DNA markers and transfer the gene to susceptible elite cultivars using the backcross breeding approach.

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Genetic analysis of sugarcane brown rust resistance genes in wild sugarcane germplasm Erianthus rockii ‘Yundian 95-19’ and Erianthus rockii ‘Yundian 95-20’

Plant Genetic Resources ◽

10.1017/s1479262119000200 ◽

2019 ◽

Vol 17 (5) ◽

pp. 460-463

Author(s):

Rong-Yue Zhang ◽

Wen-Feng Li ◽

Ying-Kun Huang ◽

Xin Lu ◽

Xiao-Yan Wang ◽

...

Keyword(s):

Genetic Analysis ◽

Rust Resistance ◽

Dominant Gene ◽

Segregation Ratio ◽

Brown Rust ◽

Inheritance Of Resistance ◽

Single Dominant Gene ◽

Sugarcane Germplasm ◽

Rust Resistance Genes ◽

Sugarcane Brown Rust

AbstractWe assessed inheritance of resistance to sugarcane brown rust (Puccinia melanocephala) in selfing F1 populations of wild sugarcane germplasm Erianthus rockii ‘Yundian 95-19’ and E. rockii ‘Yundian 95-20’. We tested parent and selfing F1 individuals for the brown rust resistance gene, Bru1, that has been shown to confer resistance to brown rust in sugarcane. The Bru1 gene was not detected in E. rockii ‘Yundian 95-19’, E. rockii ‘Yundian 95-20’ or their selfing F1 individuals, and we found there was segregation of resistance in the two selfing F1 populations (segregation ratio: 3:1). The results confirmed resistance in E. rockii ‘Yundian 95-19’ and E. rockii ‘Yundian 95-20’ to sugarcane brown rust is controlled by a novel, single dominant gene.

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Identification of Markers Linked to Seedlessness in Citrus kinokuni hort. ex Tanaka and Its Progeny Using Bulked Segregant Analysis

HortScience ◽

10.21273/hortsci.46.5.693 ◽

2011 ◽

Vol 46 (5) ◽

pp. 693-697 ◽

Cited By ~ 8

Author(s):

Dario J. Chavez ◽

José X. Chaparro

Keyword(s):

Molecular Markers ◽

Linkage Group ◽

Bulked Segregant Analysis ◽

Random Amplified Polymorphic Dna ◽

Dominant Gene ◽

Segregation Ratio ◽

Single Dominant Gene ◽

Citrus Breeding

Citrus kinokuni ‘Mukaku kishu’ PI539530 and its progeny were studied to identify random amplified polymorphic DNA (RAPD) primers associated with seedlessness. Ninety-one F1 [(Robinson op) × C. kinokuni] individuals showed a 1:1 segregation ratio between seedless and seeded phenotypes with seedless as a single dominant gene. Bulked segregant analysis was used to identify markers associated with the seedless locus. Eighteen RAPD primers were mapped into a partial linkage group (≈55.8 cM length) with four RAPD primers flanking the seedless locus: OPAI11-0.8 at 8.7 cM, OPAJ19-1.0 at 8.4 cM, OPM06r-0.85 at 4.3 cM, and OPAJ04r-0.6 at 6.4 cM. The identification of molecular markers linked to C. kinokuni Fs seedless locus constitutes an important and major tool for citrus breeding and selection.

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Resistance to Lettuce Infectious Yellows Virus in Melon

HortScience ◽

10.21273/hortsci.33.3.534b ◽

1998 ◽

Vol 33 (3) ◽

pp. 534b-534

Author(s):

James D. McCreight

Keyword(s):

Cucumis Melo ◽

Dominant Gene ◽

Field Tests ◽

Single Dominant Gene ◽

Limited Data ◽

Sweetpotato Whitefly ◽

Sources Of Resistance ◽

Lettuce Infectious Yellows Virus ◽

Greenhouse Tests

Yellowing of melon (Cucumis melo L.) incited by lettuce infectious yellows virus (LIYV) reduces yield and fruit quality of infected plants. LIYV is transmitted only by the sweetpotato whitefly (Bemisia tabaci Genn.). Two naturally infected field tests indicated several potential sources of resistance to LIYV. PI 124112 and `Snake Melon' had mild symptoms in both field tests whereas PI 313970 was asymptomatic in the test in which it was included. In greenhouse tests using controlled inoculation, PI 313970 was asymptomatic, had negative ELISA assays for LIYV, and was negative for LIYV in serial transfers to Chenopodium. `Top Mark' and `PMR 5' were symptomatic, had positive ELISA assays for LIYV, and were positive for LIYV in serial transfers to Chenopodium in these greenhouse tests. Limited data indicate that resistance in PI 313970 is conditioned by a single, dominant gene.

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Independent Resistant Reactions Expressed in Root and Tuber of Potato Breeding Lines with Introgressed Resistance to Meloidogyne chitwoodi

Phytopathology ◽

10.1094/phyto-99-9-1085 ◽

2009 ◽

Vol 99 (9) ◽

pp. 1085-1089 ◽

Cited By ~ 22

Author(s):

C. R. Brown ◽

H. Mojtahedi ◽

L.-H. Zhang ◽

E. Riga

Keyword(s):

Genetic Factors ◽

Dominant Gene ◽

Breeding Line ◽

Single Dominant Gene ◽

Solanum Bulbocastanum ◽

Chromosome 11 ◽

Meloidogyne Chitwoodi ◽

Tuber Resistance ◽

Advanced Backcross ◽

Resistance Breaking

Resistance to Meloidogyne chitwoodi was introgressed from Solanum bulbocastanum into the cultivated gene pool of potato. A single dominant gene is responsible for resistance to race 1 reproduction on the root system. An additional form of resistance was discovered in certain advanced backcross clones. A BC5 clone, PA99N82-4, resisted invasion of tubers by available nematode juveniles whether supplied by weeds or challenged by several root resistance-breaking pathotypes. This tuber resistance is inherited as a single dominant gene and is linked to RMc1(blb). Because this gene has been mapped to chromosome 11, tuber resistance genetic factors are inferred to be on the same chromosome in coupling phase. Among 153 progeny derived from crosses with PA99N82-4, 42 recombinants, comprising both resistant root/susceptible tuber and susceptible root/resistant tubers, were found while other progeny were doubly resistant (like PA99N82-4) or doubly susceptible. Therefore, the existence of two linked genetic factors controlling independently expressed traits is confirmed. The combination of the two phenotypes is likely to be a sufficient level of resistance to avoid tuber damage from circumstances that provide exogenous juveniles proximal to the tubers in the soil. These factors are weed hosts of M. chitwoodi host races and pathotypes of M. chitwoodi that overcome RMc1(blb). Under field conditions, where a resistance-breaking pathotype of M. chitwoodi was present, tuber-resistant PA99N82-4 breeding line produced tubers which were commercially acceptable and not culled. A related breeding line, root resistant but tuber susceptible, and Russet Burbank were severely tuber damaged and commercially unacceptable.

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INHERITANCE OF CROWN RUST RESISTANCE IN THREE ACCESSIONS OF AVENA STERILIS

Canadian Journal of Genetics and Cytology ◽

10.1139/g80-005 ◽

1980 ◽

Vol 22 (1) ◽

pp. 27-33 ◽

Cited By ~ 26

Author(s):

D. E. Harder ◽

R. I. H. McKenzie ◽

J. W. Martens

Keyword(s):

Rust Resistance ◽

Dominant Gene ◽

Crown Rust ◽

Inheritance Of Resistance ◽

Single Dominant Gene ◽

Avena Sterilis ◽

Oat Crown Rust ◽

Crown Rust Resistance ◽

Resistance Spectrum ◽

Dominant Genes

The inheritance of resistance to oat crown rust was studied in three accessions of Avena sterilis L. Accession CAV 4274 originated from Morocco, CAV 4540 from Algeria, and CAV 3695 from Tunisia. Seedling rust tests on F2 backcross families indicated the presence of two dominant genes for crown rust resistance in CAV 4274. One of these, a gene conditioning resistance to most races tested, was linked or allelic to gene Pc-38, and was designated gene Pc-62. The second gene conferred resistance only to one of the six races studied, and was not tested further. In CAV 4540, a single dominant gene, Pc-63 was possibly allelic with Pc-62 and linked or allelic to Pc-38. Genes Pc-62 and 63 are generally similar to Pc-38 in their resistance spectrum, but these three genes are differentiated by races CR 102, CR 103, and CR 107. A single dominant gene in CAV 3695 appeared to be Pc-50.

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THE INHERITANCE OF LOOSE SMUT RESISTANCE.: I. THE INHERITANCE OF RESISTANCE IN FOUR BARLEY VARIETIES IMMUNE TO RACE 2 OF LOOSE SMUT

Canadian Journal of Plant Science ◽

10.4141/cjps62-009 ◽

1962 ◽

Vol 42 (1) ◽

pp. 69-77 ◽

Cited By ~ 9

Author(s):

E. N. Larter ◽

H. Enns

Keyword(s):

Disease Resistance ◽

Dominant Gene ◽

The Other ◽

Inheritance Of Resistance ◽

Single Dominant Gene ◽

Loose Smut ◽

Race 2 ◽

Or Genes ◽

Dominant Genes

Four barley varieties, each immune to a Valki-attacking culture of loose smut (designated as race 2), were studied with respect to the inheritance of their resistance. Jet (C.I. 967) and Nigrinudum (C.I. 2222) were each found to possess two independent dominant genes determining resistance. Steudelli (C.I. 2266) proved to be immune to race 2 through the action of a single dominant gene, while resistance of Hillsa (C.I. 1604) was found to be conditioned by two complementary dominant genes. The absence of susceptible F3 families in crosses between Jet, Nigrinudum, and Steudelli indicated that these three varieties have in common a gene or genes for resistance to the race of smut used. The two complementary genes for resistance in Hillsa proved to be distinct from those of the other three varieties under study.The use of genetic analyses of disease resistance based upon classification of F3 families of the backcross to the resistant source is described and the merits of such a method are discussed.

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Soybean Aphid Resistance in Soybean Jackson Is Controlled by a Single Dominant Gene

Crop Science ◽

10.2135/cropsci2007.01.0003er ◽

2007 ◽

Vol 47 (1) ◽

pp. 463-463

Author(s):

Curtis B. Hill ◽

Yan Li ◽

Glen L. Hartman

Keyword(s):

Dominant Gene ◽

Soybean Aphid ◽

Aphid Resistance ◽

Single Dominant Gene

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