scholarly journals Optimization of Indole Acetic Acid Production by Pseudomonas putida UB1 and its Effect as Plant Growth-Promoting Rhizobacteria on Mustard (Brassica nigra)

2013 ◽  
Vol 2 (3) ◽  
pp. 215-221 ◽  
Author(s):  
Umang Bharucha ◽  
Kamlesh Patel ◽  
Ujjval B. Trivedi
Keyword(s):  
Acetic Acid ◽  
Plant Growth ◽  
Pseudomonas Putida ◽  
Acid Production ◽  
Indole Acetic Acid ◽  
Plant Growth Promoting Rhizobacteria ◽  
Acetic Acid Production ◽  
Indole Acetic Acid Production ◽  
Plant Growth Promoting ◽  
Growth Promoting

scholarly journals ISOLASI DAN KARAKTERISASI BAKTERI PENGHASIL IAA (INDOLE ACETIC ACID) DARI RHIZOSFER TANAMAN AKASIA (Acacia mangium)

2020 ◽  
Vol 3 (2) ◽  
pp. 210-219
Author(s):  
Ika Agus Rini ◽  
Indah Oktaviani ◽  
Muhammad Asril ◽  
Revi Agustin ◽  
Fina Khaerunissa Frima
Keyword(s):  
Acetic Acid ◽  
Plant Growth ◽  
Indole Acetic Acid ◽  
Acacia Mangium ◽  
Plant Growth Promoting Rhizobacteria ◽  
Genus Bacillus ◽  
Plant Growth Promoting ◽  
Growth Promoting

IAA adalah produk paling umum dari metabolisme L-triptofan yang dapat diproduksi oleh beberapa mikroorganisme. Beberapa mikroorganisme yang memiliki potensi menghasilkan IAA adalah bakteri rhizosfer pada tanaman Leguminosae, salah satunya adalah akasia. Acacia mangium, juga dikenal sebagai akasia, adalah pohon yang tumbuh cepat. Namun, akasia adalah tanaman invasif. Tanaman akasia memiliki bintil yang merupakan hasil simbiosis akar tanaman dan bakteri. Simbiosis ini dapat mempengaruhi kesuburan tanah. Banyak potensi yang dapat digali dari bakteri tanah, khususnya di rhizosfer. Tujuan dari penelitian ini adalah untuk mengisolasi dan mengidentifikasi bakteri pada rizosfer tanaman akasia yang mampu menghasilkan IAA sebagai salah satu potensi untuk kandidat PGPR (Plant Growth Promoting Rhizobacteria). Metode yang digunakan meliputi pengambilan sampel, isolasi bakteri penghasil IAA, pemurnian bakteri, identifikasi bakteri dan uji biokimia, pembuatan kurva tumbuha bakteri, dan uji isolat bakteri pelarut fosfat. Hasil isolasi bakteri rhizosfer diperoleh sebanyak 10 isolat bakteri yang memiliki karakteristik berbeda secara morfologi.  Berdasarkan hasil identifikasi bakteri berdasarkan pewarnaan Gram, bakteri tersebut masuk ke dalam genus Bacillus dan terdapat 5 isolat bakteri yang memiliki kemampuan menghasilkan IAA dan melarutkan fosfat sehingga bakteri tersebut memiliki potensi sebagai pupuk hayati.

scholarly journals Use of termitarium soil as a viable source for biofertilizer and biocontrol

2021 ◽  
Vol 45 (1) ◽  
Author(s):  
Seun Owolabi Adebajo ◽  
Pius Olugbenga Akintokun ◽  
Emmanuel Ezaka ◽  
Abidemi Esther Ojo ◽  
Donald Uzowulu Olannye ◽  
...  
Keyword(s):  
Acetic Acid ◽  
Plant Growth ◽  
Indole Acetic Acid ◽  
Phosphate Solubilization ◽  
Soil Samples ◽  
Production Test ◽  
Bacterial Isolates ◽  
Acetic Acid Production ◽  
Plant Growth Promoting ◽  
Growth Promoting

Abstract Background Environmental deterioration arising from the misuse of pesticides and chemical fertilizers in agriculture has resulted in the pursuit of eco-friendly means of growing crop. Evidence has shown that biofertilizers and biocontrol can boost soil fertility and suppress soil pathogens without compromising the safety of the environment. Hence, the study investigated the use of termitarium soil as a viable source for biofertilizer and biocontrol. Results Twenty-seven soil samples were collected from nine different mound soil (household, farm and water bodies in a sterile sample bag). Aliquots of serially diluted samples were plated on nutrient agar, plate count agar, eosin methylene blue agar and MacConkey agar plates. Isolates were identified using standard microbiological techniques. Identified isolates were screened for plant growth-promoting properties using phosphate solubilization test, potassium solubilization test and indole acetic acid production test. Activities of the plant growth-promoting bacteria were carried out using antagonism by diffusible substance method and antagonistic activity of cell-free culture filtrate of bacterial isolates against Ralstonia solanacearum and Fusarium oxysporum. Two hundred bacterial isolates were recovered from the 27 soil samples. The most predominant isolate was Bacillus spp. Out of the 200 bacterial isolates, 57 were positive for phosphate solubilization test, potassium solubilization test and indole acetic acid production test. Out of the 57 isolates, six bacterial isolates had antagonistic activities against Fusarium oxysporum, while seven bacterial isolates antagonized Ralstonia solanacearum. Conclusion The result showed that termite mound soil contains some useful bacteria that are capable of solubilizing phosphate and potassium and producing indole acetic acid which are the plant growth-promoting potentials and as well suppressing plant soil pathogen.

scholarly journals Isolation and characterization of novel plant growth-promoting rhizobacteria (PGPR) isolates from tomato (Solanum lycopersicum L.) rhizospherical soil: A novel IAA producing bacteria

2021 ◽  
Vol 48 (2) ◽  
Author(s):  
Narjes H. Dashti ◽  
◽  
Nedaa Y. Ali Al-Sarraf ◽  
Vineetha M. Cherian ◽  
Magdy S. Montasser ◽  
...  
Keyword(s):  
Acetic Acid ◽  
Plant Growth ◽  
Solanum Lycopersicum ◽  
Indole Acetic Acid ◽  
Plant Growth Promoting Rhizobacteria ◽  
Nucleotide Sequence Analysis ◽  
Iaa Production ◽  
Solanum Lycopersicum L ◽  
Plant Growth Promoting ◽  
Growth Promoting

Indole acetic acid (IAA) production is one of the distinctive traits of plant growth-promoting rhizobacteria (PGPR). Ten different rhizobacterial strains were isolated from the roots of tomato crops (Solanum lycopersicum L.), grown in Kuwait, and then screened for their plant growth-promoting abilities, with emphasis on IAA production. The isolated strains were identified by 16S partial r-RNA sequencing and further characterized by conventional biochemical tests. Based on the nucleotide sequence analysis and homology of the isolates, a phylogenetic tree was constructed. The plant growth-promoting abilities of the strains were determined by testing for ammonium production, hydrogen cyanide and acid production, growth in nitrogen-free media, phosphate solubilization, and IAA production. The potential IAA PGPRs were re-applied onto the rhizosphere of green-house grown tomato plants to determine their effects on tomato growth. The results have indicated that all isolated strains were able to produce indole acetic acid even without the presence of a tryptophan precursor.

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