scholarly journals Isolation and characterization of novel plant growth-promoting rhizobacteria (PGPR) isolates from tomato (Solanum lycopersicum L.) rhizospherical soil: A novel IAA producing bacteria

2021 ◽  
Vol 48 (2) ◽  
Author(s):  
Narjes H. Dashti ◽  
◽  
Nedaa Y. Ali Al-Sarraf ◽  
Vineetha M. Cherian ◽  
Magdy S. Montasser ◽  
...  
Keyword(s):  
Acetic Acid ◽  
Plant Growth ◽  
Solanum Lycopersicum ◽  
Indole Acetic Acid ◽  
Plant Growth Promoting Rhizobacteria ◽  
Nucleotide Sequence Analysis ◽  
Iaa Production ◽  
Solanum Lycopersicum L ◽  
Plant Growth Promoting ◽  
Growth Promoting

Indole acetic acid (IAA) production is one of the distinctive traits of plant growth-promoting rhizobacteria (PGPR). Ten different rhizobacterial strains were isolated from the roots of tomato crops (Solanum lycopersicum L.), grown in Kuwait, and then screened for their plant growth-promoting abilities, with emphasis on IAA production. The isolated strains were identified by 16S partial r-RNA sequencing and further characterized by conventional biochemical tests. Based on the nucleotide sequence analysis and homology of the isolates, a phylogenetic tree was constructed. The plant growth-promoting abilities of the strains were determined by testing for ammonium production, hydrogen cyanide and acid production, growth in nitrogen-free media, phosphate solubilization, and IAA production. The potential IAA PGPRs were re-applied onto the rhizosphere of green-house grown tomato plants to determine their effects on tomato growth. The results have indicated that all isolated strains were able to produce indole acetic acid even without the presence of a tryptophan precursor.

scholarly journals ISOLASI DAN KARAKTERISASI BAKTERI PENGHASIL IAA (INDOLE ACETIC ACID) DARI RHIZOSFER TANAMAN AKASIA (Acacia mangium)

2020 ◽  
Vol 3 (2) ◽  
pp. 210-219
Author(s):  
Ika Agus Rini ◽  
Indah Oktaviani ◽  
Muhammad Asril ◽  
Revi Agustin ◽  
Fina Khaerunissa Frima
Keyword(s):  
Acetic Acid ◽  
Plant Growth ◽  
Indole Acetic Acid ◽  
Acacia Mangium ◽  
Plant Growth Promoting Rhizobacteria ◽  
Genus Bacillus ◽  
Plant Growth Promoting ◽  
Growth Promoting

IAA adalah produk paling umum dari metabolisme L-triptofan yang dapat diproduksi oleh beberapa mikroorganisme. Beberapa mikroorganisme yang memiliki potensi menghasilkan IAA adalah bakteri rhizosfer pada tanaman Leguminosae, salah satunya adalah akasia. Acacia mangium, juga dikenal sebagai akasia, adalah pohon yang tumbuh cepat. Namun, akasia adalah tanaman invasif. Tanaman akasia memiliki bintil yang merupakan hasil simbiosis akar tanaman dan bakteri. Simbiosis ini dapat mempengaruhi kesuburan tanah. Banyak potensi yang dapat digali dari bakteri tanah, khususnya di rhizosfer. Tujuan dari penelitian ini adalah untuk mengisolasi dan mengidentifikasi bakteri pada rizosfer tanaman akasia yang mampu menghasilkan IAA sebagai salah satu potensi untuk kandidat PGPR (Plant Growth Promoting Rhizobacteria). Metode yang digunakan meliputi pengambilan sampel, isolasi bakteri penghasil IAA, pemurnian bakteri, identifikasi bakteri dan uji biokimia, pembuatan kurva tumbuha bakteri, dan uji isolat bakteri pelarut fosfat. Hasil isolasi bakteri rhizosfer diperoleh sebanyak 10 isolat bakteri yang memiliki karakteristik berbeda secara morfologi.  Berdasarkan hasil identifikasi bakteri berdasarkan pewarnaan Gram, bakteri tersebut masuk ke dalam genus Bacillus dan terdapat 5 isolat bakteri yang memiliki kemampuan menghasilkan IAA dan melarutkan fosfat sehingga bakteri tersebut memiliki potensi sebagai pupuk hayati.

scholarly journals Extraction of Indole-3-acetic Acid from Plant Growth Promoting Rhizobacteria of Bamboo Rhizosphere and Its Effect on Biosynthesis of Chlorophyll in Bamboo Seedlings

2020 ◽  
Author(s):  
Janardan Lamichhane ◽  
Bishnu Maya K.C. ◽  
Dhurva Prasad Gauchan ◽  
Sanjay Nath Khanal ◽  
Sharmila Chimouriya
Keyword(s):  
Acetic Acid ◽  
Plant Growth ◽  
Biochemical Analysis ◽  
Chlorophyll Biosynthesis ◽  
Plant Growth Promoting Rhizobacteria ◽  
Agitation Rate ◽  
Iaa Production ◽  
Plant Growth Promoting ◽  
Growth Promoting ◽  
Indole 3 Acetic Acid

Background: Indole-3-acetic acid (IAA), a principal phytohormone, controls several crucial physiological processes of plants. It ameliorates plant growth by stimulating cell elongation, root initiation, seed germination and seedling growth. Alteration of IAA level by plant growth promoting rhizobacteria leads to varied impacts on plant growth and development. Methods: Soil samples were collected from bamboo (Bambusa tulda, B. nutans subsp. cupulata, B. balcooa and Dendrocalamus strictus) rhizosphere. Altogether five bacterial isolates were screened by serial dilution method and subjected to biochemical analysis. The isolate BUX1 with high IAA production capacity was optimized for IAA production. IAA was partially purified and quantified from the bacterial extract by thin layer chromatography (TLC). The influence of extracted bacterial IAA on chlorophyll biosynthesis in bamboo seedlings of B. tulda was compared with uninoculated control plants. Results: Biochemical analysis revealed that all the isolates belonged to genus Bacillus which were found capable of producing IAA. During optimization, BUX1 isolate produced 99.13 µg ml-1 of IAA at 37°C, pH 7, 3 mg l-1 concentration of L-tryptophan and 150 rpm agitation rate after 192 hour of incubation. The Rf value of the bacterial IAA during TLC was identical to that of standard IAA (0.425) indicating that IAA was present in crude extract of Bacillus (BUX1). The influence of bacterial IAA on chlorophyll biosynthesis in bamboo seedlings was significant in comparison to uninoculated plants. Therefore, this isolate could be a prospective candidate to be employed as biofertilizer.

scholarly journals Use of plant growth promoting rhizobacteria (PGPRs) in the mitigation of water deficiency of tomato plants (Solanum lycopersicum L.)

2021 ◽  
Vol 22 (1) ◽  
pp. 167-177
Author(s):  
Andras Nemenyi ◽  
Bulgan ANDRYEI ◽  
Kitti Zsuzsanna HORVÁTH ◽  
Stella AGYEMANG DUAH ◽  
Sándor TAKÁCS ◽  
...  
Keyword(s):  
Plant Growth ◽  
Solanum Lycopersicum ◽  
Plant Growth Promoting Rhizobacteria ◽  
Water Deficiency ◽  
Tomato Plants ◽  
Solanum Lycopersicum L ◽  
Plant Growth Promoting ◽  
Growth Promoting
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