Fungi of the genus Fusarium, known as toxigenic species, are very of- ten parasites and contaminants of brewer's barley. In this paper, the composition of the genus Fusarium species in brewer's barley samples and their potential in the zearalenone synthesis were investigated. The tests were done on different brewer's barley varieties, crop 2003, samples (SSK1, SSK2, SSK3 SSK4, SSK5, SSK6, SSK7, SSK8, SSK9, SSK10 and SSK12) from Kragujevac locality. The isolation and identification of the Fusarium species were done according to the methods described by N e l s o n et al. (1983). The identified Fusarium species (6) were tested for their capacity to synthesise zearalenone. The isolates were cultivated on sterilised barley grains at the temperature of 25?C for 14 days, and then the zearalenone concentration was determined by the fluorometric method on the fluorometer "VI- CAM" series 4. The following seven Fusarium species were isolated from barley samples: F. acuminatum, F. avenaceum, F. culmorum, F. equiseti, F. poae, F. sporotrichioides and F. tricinctum. F. poae was the most distributed species (10.26%). The zearalenone concentration within the range of 12.0 to 430.0 g kg-1 was determined in cultures of barley grain inoculated with F. avenacuem (SSK6 and SSK12), F. culmorum (SSK8), F. tricinctum (SSK1), F. sporotrichioides (SSK7 and SSK12) and F. poae (SSK5, SSK9 and SSK10). Isolates of F. equiseti (SSK2) and F. poae (SSK6) did not express capacity to synthesise this toxic metabolite.