Biochemical and molecular evaluation of pigeonpea against Fusarium wilt

The present study aimed to estimate the efficiency of both a cellular bovine pericardium and bovine urinary bladder matrix sheets in the reconstruction of large ventro-lateral hernias in Iraqi bucks by using of molecular evaluation depending on real time-polymerase chain reaction technique to investigate the level of basic-fibroblast growth factor and vascular endothelial growth factor genes during the healing process and reconstruction of the abdominal defects. Under sedation and local anesthesia, (6cm X 8cm size) of ventro-lateral hernias were induced in 24 of Iraqi bucks. The animals were divided randomly into two main equal groups. In bovine pericardium-treatment group, the hernias were treated with onlay implantation of bovine pericardium. While, the hernias in UBM-treatment group were treated with onlay implantation of urinary bladder matrix, 30 days post-inducing of hernias. The molecular evaluation along the period of following-up recorded a significant up-regulation of the level of basic-fibroblast growth factor gene specific for presence of fibroblasts, myofibroblasts and collagen deposition in urinary bladder matrix -treatment group in comparison to bovine pericardium -treatment group with significant difference even at the end of the study. While, a significant up regulation of the levels of angiogenesis classic gene vascular endothelial growth factor were recorded in the bucks of bovine pericardium -treatment group compared to urinary bladder matrix -treatment group. In conclusion; molecular detection of the level of growth factors in target tissue can be used as an important criterion.

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MORPHOLOGICAL AND MOLECULAR EVALUATION OF THE FAR EAST FRUIT GENETIC RESOURCES OF LONICERA CAERULEA L.

PROCEEDINGS ON APPLIED BOTANY GENETICS AND BREEDING ◽

10.30901/0202-3628-2015-3-325-335 ◽

2015 ◽

Vol 176 (3) ◽

pp. 325-335

Author(s):

V. Holubec ◽

◽

T. Smekalova ◽

L. Leisova–Svobodova ◽

◽

...

Keyword(s):

Genetic Resources ◽

Far East ◽

Lonicera Caerulea ◽

The Far East ◽

Fruit Genetic Resources ◽

Molecular Evaluation

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Analysis and Visualisation of Research Trends in Fusarium Wilt

International Journal of Pharmaceutical Research ◽

10.31838/ijpr/2020.sp3.022 ◽

2020 ◽

Vol 12 (sp3) ◽

Keyword(s):

Fusarium Wilt ◽

Research Trends

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Uji Toksisitas Beberapa Fungisida Nabati terhadap Penyakit Layu Fusarium (Fusarium oxysporum) pada Tanaman Kentang (Solanum tuberosum L.) secara In Vitro (Toxicity Test of several Biofungicides in controlling Fusarium wilt (Fusarium oxysporum) in Potato Plants (Solanum tuberosum L.) by In Vitro)

JURNAL BIOS LOGOS ◽

10.35799/jbl.9.2.2019.24416 ◽

2019 ◽

Vol 9 (2) ◽

pp. 91

Author(s):

Ghea Dotulong ◽

Stella Umboh ◽

Johanis Pelealu

Keyword(s):

Solanum Tuberosum ◽

Fusarium Oxysporum ◽

Fusarium Wilt ◽

Leaf Extract ◽

Solanum Tuberosum L ◽

In Vitro Toxicity ◽

Potato Plants ◽

Colony Diameter ◽

In Vitro Toxicity Test

Uji Toksisitas Beberapa Fungisida Nabati terhadap Penyakit Layu Fusarium (Fusarium oxysporum) pada Tanaman Kentang (Solanum tuberosum L.) secara In Vitro (Toxicity Test of several Biofungicides in controlling Fusarium wilt (Fusarium oxysporum) in Potato Plants (Solanum tuberosum L.) by In Vitro) Ghea Dotulong1*), Stella Umboh1), Johanis Pelealu1), 1) Program Studi Biologi, FMIPA Universitas Sam Ratulangi, Manado 95115*Email korespondensi: [email protected] Diterima 9 Juli 2019, diterima untuk dipublikasi 10 Agustus 2019 Abstrak Tanaman kentang (Solanum tuberosum L.) adalah salah satu tanaman hortikultura yang sering mengalami penurunan dari segi produksi dan produktivitasnya, akibat adanya serangan penyakit layu yang salah satunya disebabkan oleh Fusarium oxysporum. Tujuan penelitian ini adalah mengidentifikasi toksisitas beberapa fungisida nabati dalam mengendalikan penyakit Layu Fusarium (F. oxysporum) pada tanaman kentang (Solanum tuberosum L.) secara In Vitro. Metode Penelitian yang digunakan yaitu metode umpan beracun. Data dianalisis dengan Rancangan Acak Lengkap (RAL) dengan Analisis Varian (ANAVA) yang dilanjutkan dengan menggunakan metode BNT (Beda Nyata Terkecil). Hasil Penelitian, diperoleh nilai toksisitas fungisida nabati tertinggi yaitu pada ekstrak daun sirsak dengan nilai HR (69,44%), kategori berpengaruh, ditandai dengan diameter koloni 2,75 cm (100ppm) dan yang terendah toksisitasnya yaitu pada ekstrak daun jeruk purut dengan nilai HR (49,81%), kategori cukup berpengaruh ditandai dengan diameter koloni 3,75 cm (25ppm). Semakin tinggi konsentrasi yang diujikan maka semakin tinggi toksisitas dari fungisida nabati yang diberikan.Kata Kunci: fungisida nabati, Fusarium oxysporum, tanaman kentang, In Vitro Abstract Potato plants (Solanum tuberosum L.) is one of the horticulture plants which often decreases in terms of production and productivity, due to the attack of wilt, one of which is caused by Fusarium oxysporum. The purpose of this study was to determine the toxicity of several biofungicides in controlling Fusarium wilt (F. oxysporum) in potato plants (Solanum tuberosum L.) in Vitro. The research method used was the In Vitro method with the poison bait method. Data were analyzed by Completely Randomized Design with Variant Analysis (ANAVA), followed by the BNT method. The results showed that the highest biofungicide toxicity value was soursop leaf extract with HR values (69.44%), influential categories, characterized by colony diameter 2.75 cm (100ppm) and the lowest toxicity, namely in kaffir lime leaf extract with a value of HR (49.81%), quite influential category was characterized by colony diameter of 3.75 cm (25ppm). The higher the concentration tested, the higher the toxicity of the biofungicide given.Keywords: biofungicides, Fusarium oxysporum, Potato Plants, In Vitro.

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