Evaluation of Scab and Mildew Resistance in the Gene Bank Collection of Apples in Dresden-Pillnitz

A set of 680 apple cultivars from the Fruit Gene bank in Dresden Pillnitz was evaluated for the incidence of powdery mildew and scab in two consecutive years. The incidence of both scab and powdery mildew increased significantly in the second year. Sixty and 43 cultivars with very low incidence in both years of scab and powdery mildew, respectively, were analysed with molecular markers linked to known resistance genes. Thirty-five cultivars were identified to express alleles or combinations of alleles linked to Rvi2, Rvi4, Rvi6, Rvi13, Rvi14, or Rvi17. Twenty of them, modern as well as a few traditional cultivars known before the introduction or Rvi6 from Malus floribunda 821, amplified the 159 bp fragment of marker CH_Vf1 that is linked to Rvi6. Alleles linked to Pl1, Pld, or Plm were expressed from five cultivars resistant to powdery mildew. Eleven cultivars were identified to have very low susceptibility to both powdery mildew and scab. The information on resistance/susceptibility of fruit genetic resources towards economically important diseases is important for breeding and for replanting traditional cultivars. Furthermore, our work provides a well-defined basis for the discovery of undescribed, new scab, and powdery mildew resistance.

Diversity of Native and Exotic Fruit Genetic Resources in Nepal

Diversity in fruit genetic resources in Nepal is contributed by wild, indigenous and exotic sources. This study was carried out to bring together the available fruit species and cultivars at various stations of Department of Agriculture (DoA), Nepal Agricultural Research Council (NARC), Agriculture and Forest University (AFU) and private farms until the Fiscal Year 2017/2018. Altogather there were 47 species of fruits from tropical zone of Terai (Tarahara, Janakpur, Sarlahi, Parwanipur and Khajura) to cold temperature zone of high hills (Marpha, Rajikot and Satbanj) across the country. Apple diversity was found at Horticulture Research Station, Rajikot, Jumla and has introduced 25 spur type cultivars. National Citrus Research Programme (NCRP), Dhankuta was citrus most diversity areas and has maintained 130 exotic and indigenous germplasms of citrus species followed by NCFD, Kirtipur. Mango diversity was noted at RARS, Tarahara (16 cultivars), RARS, Parwanipur (25 cultivars), Farm of DoA-Sarlahi (30 cultivars), Farms of DoA-Janakpur (18 cultivars), AFU-Rampur (17 cultivars). Some of the private nurseries like Everything Organic Nursery, Patlekhet, Kavre and international organization like Technology Demonstration Centre of ICIMOD, Godawari, Lalitpur were also found to be a diversity centre of many exotic and indigenous germplasms of fruit species. These indigenous fruit genetic resources were also used to develop varieties such as Sunkagati-1 and Sunkagati-2 and Tehrathum Local of acid lime, Khoku Local of mandarin orange, 'Malbhog' of banana which were notified by the National Seed Board, SQCC. The unique fruit genetic resources were ‘Pharping Local’ (Asian sand pear), ‘Sindhuli Junar’ (sweet orange), ‘Dhankuta Local’ and ‘Manakamana Local’ (mandarin), Local Malbhog (banana), Bhaktapure Lapsi (Nepalese hog plum) etc. which have superior traits than exotic fruits. Unique and wild fruit species were yellow, black and red raspberries (Rubus ellipticus, R. foliolosus and R. acuminatus respectively), bale (Aegle marmelos), pummelo (Citrus grandis), citron (Citrus medica), sweet lime (Citrus limettoides), butter tree or chiuri (Basia buttyacea), tamarind (Tamarindus indica), black plum (Syzygium cumini), wild apple (Mallus baccata), rough lemon (Citrus jambhiri), bayberry (Myrica esculanta), edimayal (Pyrus pashia), black and white ebony (Diospyrus malbarica), wild species of olive (Olea ferruginea and O. glandulifera), wild kiwifruit (Actinidia callosa) etc. Most of the diversity studies were based on phenotypic descriptions. We believe that the number of species and genotypes listed in this article would be increased if detail survey is further carried out. Way forward to utilize these valuable genetic resources has also been discussed in this manuscript.

Identifikasi dan Karakterisasi Sumber Daya Genetik Buah-buahan di Kabupaten Buleleng

Identification and Characterization of Fruit Genetic Resources in Buleleng Regency. Fruits have great potential to be developed both to meet the needs of domestic and foreign demand, because the fruits is a source of supply of vitamins, minerals, and fiber needed in the daily menu. Several types of fruit are also required as spa, medicines, and as a means of upakara. One of the prospective development of fruits in Bali is Buleleng. Types of fruit in Buleleng are grape, mango, durian, rambutan and banana. Until now there has been generally identified genetic resources fruits in Buleleng. This study aims to identify, profile and draw up a map of the geographic distribution of genetic resources of fruits in Buleleng. Implementation of the study consists of four activities, namely: secondary data collection, survey of the types of genetic resources and distribution, identification of morphological and agronomic characters of each type of genetic resources and the growing environment and the benefits of fruits. The seeded fruit is determined by Location Quotion (LQ). Based on the observations in the field, Buleleng has a wide range of genetic resources. In a direct observation to the field there are 32 kinds of fruit and 31 sub-types of fruit. Fruits are grouped based on the economic value and their role, including: commercial fruit, rare fruit, fruit for upakara and fruit for medicine. Based on the calculation of LQ method, Buleleng has four featured i.e. avocado, grape, mango and rambutan (LQ>1).