Repairing mechanism of foliar micro-morphological anomalies during acclimatization and field transfer of in vitro raised plantlets of Aerva lanata (L.) Juss. ex Schult.: a medicinally important plant

2021 ◽  
Author(s):  
M. Manokari ◽  
S. Priyadharshini ◽  
Mahipal S. Shekhawat
Keyword(s):  
Morphological Anomalies ◽  
Aerva Lanata

scholarly journals Leaf Extracts of Aerva lanata Inhibit the Activities of Type 2 Diabetes-Related Enzymes and Possess Antioxidant Properties

2018 ◽  
Vol 2018 ◽  
pp. 1-7 ◽  
Author(s):  
Musbau Adewunmi Akanji ◽  
Samson Olasunkanmi Olukolu ◽  
Mutiu Idowu Kazeem
Keyword(s):  
Antioxidant Activities ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Leaf Extracts ◽  
Chemically Induced ◽  
Radical Scavenging Ability ◽  
Aerva Lanata ◽  
Radical Scavenging Properties

The leaves of Aerva lanata are one of the indigenous medicinal plants used in the management of diabetes mellitus and its associated complications in Africa. However, its effect on the activities of diabetes-related enzymes has not been investigated. This study evaluated the in vitro inhibitory effects of different extracts of the A. lanata leaf on the activities of diabetes-related enzymes (α-amylase and α-glucosidase) and chemically induced free radicals. Aqueous, ethanol, and hydroethanol extracts of A. lanata leaves were subjected to a standard enzyme inhibition assay followed by determination of modes of inhibition of the enzymes. The antioxidant activities of the extracts were evaluated using 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 2,2-azino-bis-(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS). The results obtained showed that the hydroethanol extract of the A. lanata leaf optimally inhibited both α-amylase (IC50: 2.42 mg/mL) and α-glucosidase (IC50: 0.23 mg/mL). The Lineweaver-Burk plot revealed that the mode of inhibition of both enzymes by the hydroethanol extract was uncompetitive. However, the hydroethanol and aqueous extracts displayed the best DPPH and ABTS radical-scavenging ability, respectively. It can be concluded that the A. lanata extract inhibited the activities of both α-amylase and α-glucosidase uncompetitively, which may be attributed to its free radical-scavenging properties and rich phenolic composition.

scholarly journals Changes in the content of carotenoids and flavonoids in medicinal raw material of Digitalis purpurea, Polemonium caeruleum, and Aerva lanata, cultivated in vitro under the chronic action of ionizing radiation

2021 ◽  
Vol 22 (1) ◽  
pp. 85-92
Author(s):  
S.V. Litvinov ◽  
◽  
L.G. Lioshyna ◽  
O.V. Bulko ◽  
K.V. Lystvan ◽  
...  
Keyword(s):  
Low Dose ◽  
Raw Material ◽  
Small Range ◽  
Γ Irradiation ◽  
Digitalis Purpurea ◽  
Dose Irradiation ◽  
Post Radiation ◽  
Aerva Lanata ◽  
The Individual

Chronic low-dose γ-irradiation of Digitalis purpurea and Aerva lanata plants in vitro have led to an increase in the content of carotenoids and flavonoids in the leaves at the beginning of the post-radiation period. In the following, the content of pigments and flavonoids decreased in comparison to the control samples. For Polemonium caeruleum such changes had been not observed. The qualitative composition of the secondary metabolites has not been changed, but the ratio of the individual components of the extract varied within a small range. Ri-transformants of Digitalis purpurea have been shown smaller changes in response to irradiation. We assume that the observed effects are the manifestation of the adaptive response of plants to oxidative stress caused by chronic low-dose irradiation.

Mitigating in vitro induced macro- and micro-morphological anomalies using seismic stress in Vitex negundo L.

2020 ◽  
Vol 143 (2) ◽  
pp. 411-420 ◽  
Author(s):  
M. Manokari ◽  
S. Priyadharshini ◽  
N. Arumugam ◽  
Mahipal S. Shekhawat
Keyword(s):  
Vitex Negundo ◽  
Morphological Anomalies ◽  
Seismic Stress

scholarly journals An Improved liquid Culture System for Efficient Shoot Multiplication in Aerva lanata (L.) Juss. Ex Schult.

2021 ◽  
Vol 31 (1) ◽  
pp. 35-42
Author(s):  
Kandhan Varutharaju ◽  
Chandrasekaran Thilip ◽  
Palusamy Raja ◽  
Ganesan Thiagu ◽  
Abubakker Aslam ◽  
...  
Keyword(s):  
Liquid Medium ◽  
Solid Medium ◽  
Shoot Proliferation ◽  
Shoot Multiplication ◽  
Multiplication Rate ◽  
Mass Propagation ◽  
Liquid Culture System ◽  
Aerva Lanata ◽  
Better Than

An improved in vitro mass propagation protocol was developed for Aerva lanata using MS liquid medium. The influence of MS medium (solid and liquid) with cytokinin (TDZ and BAP, respectively) were studied for shoot proliferation and growth. The liquid medium perfomed better than solid medium in shoot multiplication. The maximum shoot multiplication rate was (29.37 ± 0.64 shoots per explant), obtained in MS liquid medium which is containing 0.6 mg/l TDZ, 0.3 mg/l NAA and 0.2 mg/l IBA. Different volumes of liquid medium have been used, 30 ml of medium flask showed the maximum number of shoots. Liquid medium is better suited for in vitro propagation of A. lanata since the enhanced multiplication rate was observed with shorter subculture intervals. Plant Tissue Cult. & Biotech. 31(1): 35-42, 2021 (June)

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