Mitigating in vitro induced macro- and micro-morphological anomalies using seismic stress in Vitex negundo L.

2020 ◽  
Vol 143 (2) ◽  
pp. 411-420 ◽  
Author(s):  
M. Manokari ◽  
S. Priyadharshini ◽  
N. Arumugam ◽  
Mahipal S. Shekhawat
Keyword(s):  
Vitex Negundo ◽  
Morphological Anomalies ◽  
Seismic Stress

Micro-Structural Stability of Micropropagated Plants of Vitex negundo L.

2021 ◽  
pp. 1-9
Author(s):  
M. Manokari ◽  
S. Priyadharshini ◽  
Mahipal S. Shekhawat
Keyword(s):  
Structural Changes ◽  
Growth Conditions ◽  
Culture Industry ◽  
Vascular Bundles ◽  
Vitex Negundo ◽  
Structural Variations ◽  
Structural Repair ◽  
Micropropagated Plants ◽  
Palisade Cells

Abstract Micropropagation techniques allow producing large numbers of clones of genetically identical plants. However, there is evidence of disorders in internal structures due to sophisticated in vitro conditions. Such variations are responsible for the mortality of plantlets in the field and cause huge loss to the tissue culture industry. Anatomical evaluation at different growth conditions allows for understanding structural repair of in vitro raised plantlets. Therefore, the present study was aimed to identify the structural changes that occurred in micropropagated plants of Vitex negundo under heterotrophic, photomixotrophic, and photoautotrophic conditions. To achieve this, structural variations were analyzed in the plantlets obtained from in vitro, greenhouse and field transferred stages using light microscopy. Underdeveloped dermal tissues, palisade cells, intercellular spaces, mechanical tissues, vascular bundles, and ground tissues were observed with the plants growing under in vitro conditions. The self-repairing of structural disorders and transitions in vegetative anatomy was observed during hardening under the greenhouse environment. Field transferred plantlets were characterized by well-developed internal anatomy. These findings showed that the micropropagated plantlets of V. negundo were well-adapted through a series of self-repairing the in vitro induced structural abnormalities at the subsequent stages of plant development.

Biotechnological Approaches for In-Vitro Propagation of a Valuable Medicinal Plant - Vitex Negundo L

2021 ◽  
Vol 12 (3) ◽  
pp. 126-132
Author(s):  
Mahakur Bhaswatimayee ◽  
Begum Rosanara ◽  
Barik Durga Prasad
Keyword(s):  
Medicinal Plant ◽  
In Vitro Propagation ◽  
Vitex Negundo

Green Synthesis of Gold Nanoparticles from Vitex negundo Leaf Extract to Inhibit Lipopolysaccharide-Induced Inflammation Through In Vitro and In Vivo

2019 ◽  
Vol 31 (2) ◽  
pp. 463-477 ◽  
Author(s):  
Ningaraju Sunayana ◽  
Munawer Uzma ◽  
Rudra Prasad Dhanwini ◽  
Mellappa Govindappa ◽  
Harischandra Sripathy Prakash ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Green Synthesis ◽  
Leaf Extract ◽  
Vitex Negundo

Evaluation of ethnopharmacologically selected Vitex negundo L. for In vitro antimalarial activity and secondary metabolite profiling

2021 ◽  
Vol 275 ◽  
pp. 114076
Author(s):  
Manish Kumar Dwivedi ◽  
Ravindra Shukla ◽  
Naveen Kumar Sharma ◽  
Ashan Manhas ◽  
Kumkum Srivastava ◽  
...  
Keyword(s):  
Secondary Metabolite ◽  
Metabolite Profiling ◽  
Antimalarial Activity ◽  
Vitex Negundo

scholarly journals SEED MYCOFLORA OF Lens culinaris MEDDIK. FROM DIFFERENT STORAGE CONDITIONS AND IN-VITRO INHIBITION USING PLANT EXTRACTS

2016 ◽  
Vol 23 ◽  
pp. 19-27 ◽  
Author(s):  
Roshan Chhetri ◽  
Sanjay Kumar Jha
Keyword(s):  
Aspergillus Niger ◽  
Fusarium Oxysporum ◽  
Alternaria Alternata ◽  
Lens Culinaris ◽  
Agricultural Research ◽  
Research Centre ◽  
Local Market ◽  
Vitex Negundo ◽  
Eclipta Prostrata

Nine hundred sixty seeds of lentils (Lens culinaris) from three different storage sources were tested for seed fungi using Potato Dextrose Agar (PDA) and Blotter technique. Thirty five different fungi were isolated, PDA method being better than Blotter technique. Seeds from traditional village storage were found to be more infested with fungi than source local market and source Nepal Agricultural Research Centre (NARC). The fungal infestation on seeds from village storage was 49.37%, source NARC was 44.37% and source local market was 30.31%. Aspergillus flavus, Aspergillus niger, Alternaria alternata, Penicillium chrysogenum and Fusarium oxysporum were the fungi having higher incidence and frequency. Surface disinfection by 2% NaOCl reduced the fungal incidence and frequency. The aqueous extract of three plants viz, Ageratum houstonianum, Eclipta prostrata and Vitex negundo at 25%, 50% and 100% concentration were assessed in-vitro for antifungal activity against Alternaria alternata, Aspergillus niger, Chaetomium funicola and Fusarium oxysporum. Among these fungi, Alternaria alternata was best controlled by Ageratum houstonianum; Aspergillus niger by Eclipta prostrata; Fusarium oxysporum by Vitex negundo and Ageratum houstonianum. The inhibition increased from lower concentration (25%) to higher (100%).ECOPRINT 23: 19-27, 2016

scholarly journals In Vitro Regeneration of Vitex negundo L., a Woody Valuable Medicinal Plant through High Frequency Axillary Shoot Proliferation

1970 ◽  
Vol 43 (3) ◽  
pp. 345-352 ◽  
Author(s):  
Farhana Afroz ◽  
AKM Sayeed Hassan ◽  
Laila Shamroze Bari ◽  
Rebeka Sultana ◽  
John Liton Munshi ◽  
...  
Keyword(s):  
Medicinal Plant ◽  
Large Scale ◽  
Shoot Proliferation ◽  
Shoot Multiplication ◽  
Multiple Shoots ◽  
Nodal Segments ◽  
Axillary Shoot ◽  
Vitex Negundo ◽  
Axillary Shoot Proliferation

An efficient protocol was established for rapid and large scale propagation of woody aromatic medicinal plant Vitex negundo L. by in vitro shoot multiplication from shoot tips and nodal segments of mature plant. Of the four different growth regulators BA, Kn, GA3, NAA and coconut water, MS fortified with BA 1.0 mg/l was found to be the most effective for inducing multiple shoots from nodal explants. The percentage (96%) of shoot multiplication per node (21.83) was highest up to second subculture passages, after which there was a gradual decline in shoot development. Best rooting was induced (93%) in excised shoots on half strength MS medium supplemented with an optimal combination of NAA (0.3 mg/l). Soil, compost and sand (1:1:1) mixture was the most suitable planting substrate for hardening. The survival rate was 80% and the regenerated plants were successfully transferred to the soil.Key words: Vitex negundo, Medicinal plant, Shoot proliferation, Micropropagation, RegenerationDOI = 10.3329/bjsir.v43i3.1149Bangladesh J. Sci. Ind. Res. 43(3), 345-352, 2008

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