kdr type mutations and enhanced GST based insecticide resistance in dengue vector mosquitoes Aedes aegypti and Aedes albopictus

Background In Malaysia, dengue remains a top priority disease and usage of insecticides is the main method for dengue vector control. Limited baseline insecticide resistance data in dengue hotspots has prompted us to conduct this study. The present study reports the use of a map on the insecticide susceptibility status of Aedes aegypti and Aedes albopictus to provide a quick visualization and overview of the distribution of insecticide resistance. Method and results The insecticide resistance status of Aedes populations collected from 24 dengue hotspot areas from the period of December 2018 until June 2019 was proactively monitored using the World Health Organization standard protocol for adult and larval susceptibility testing was conducted, together with elucidation of the mechanisms involved in observed resistance. For resistance monitoring, susceptibility to three adulticides (permethrin, deltamethrin, and malathion) was tested, as well as susceptibility to the larvicide, temephos. Data showed significant resistance to both deltamethrin and permethrin (pyrethroid insecticides), and to malathion (organophosphate insecticide) in all sampled Aedes aegypti populations, while variable resistance patterns were found in the sampled Aedes albopictus populations. Temephos resistance was observed when larvae were tested using the diagnostic dosage of 0.012mg/L but not at the operational dosage of 1mg/L for both species. Conclusion The present study highlights evidence of a potential threat to the effectiveness of insecticides currently used in dengue vector control, and the urgent requirement for insecticide resistance management to be integrated into the National Dengue Control Program.

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Insecticide resistance in the major dengue vectors Aedes albopictus and Aedes aegypti

Pesticide Biochemistry and Physiology ◽

10.1016/j.pestbp.2012.05.008 ◽

2012 ◽

Vol 104 (2) ◽

pp. 126-131 ◽

Cited By ~ 188

Author(s):

J. Vontas ◽

E. Kioulos ◽

N. Pavlidi ◽

E. Morou ◽

A. della Torre ◽

...

Keyword(s):

Aedes Aegypti ◽

Insecticide Resistance ◽

Aedes Albopictus ◽

Dengue Vectors

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Exploring the molecular basis of insecticide resistance in the dengue vector Aedes aegypti: a case study in Martinique Island (French West Indies)

BMC Genomics ◽

10.1186/1471-2164-10-494 ◽

2009 ◽

Vol 10 (1) ◽

pp. 494 ◽

Cited By ~ 108

Author(s):

Sébastien Marcombe ◽

Rodolphe Poupardin ◽

Frederic Darriet ◽

Stéphane Reynaud ◽

Julien Bonnet ◽

...

Keyword(s):

Aedes Aegypti ◽

Insecticide Resistance ◽

West Indies ◽

Molecular Basis ◽

Dengue Vector ◽

French West Indies

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Insecticide resistance of Dengue vectors in South East Asia: a systematic review

African Health Sciences ◽

10.4314/ahs.v21i3.21 ◽

2021 ◽

Vol 21 (3) ◽

pp. 1124-1140

Author(s):

Mohd Rohaizat Hassan ◽

Noor Atika Azit ◽

Suhaiza Mohd Fadzil ◽

Siti Rasidah Abd Ghani ◽

Norfazilah Ahmad ◽

...

Keyword(s):

Aedes Aegypti ◽

Insecticide Resistance ◽

East Asia ◽

Aedes Albopictus ◽

Pyrethroid Resistance ◽

Resistance Management ◽

Genetic Mutation ◽

Peninsular Malaysia ◽

South East Asia ◽

Dengue Vectors

Background: The insecticides used widely has led to resistance in the vector and impose a challenge to vector control op- eration. Objectives: This review aims to analyse the distribution of insecticide resistance of dengue vectors in South East Asia and to describe the mechanism of insecticide resistance. Methods: Literature search for articles published on 2015 to 2019 from PubMed, Scopus and ProQuest was performed. Total of 37 studies included in the final review from the initial 420 studies. Results: Pyrethroid resistance was concentrated on the west coast of Peninsular Malaysia and Northern Thailand and scat- tered at Java Island, Indonesia while organophosphate resistance was seen across the Java Island (Indonesia), West Sumatera and North Peninsular Malaysia. Organochlorine resistance was seen in Sabah, Malaysia and scattered distribution in Nusa Tenggara, Indonesia. V1016G, S989P, F1269C gene mutation in Aedes Aegypti were associated with Pyrethroid resistance in Singapore and Indonesia. In Malaysia, over-expressed with monooxygenase P450 genes (CYP9J27, CYP6CB1, CYP9J26 and CYP9M4) Glutathione S-transferases, carboxylesterases commonly associated with pyrethroids resistance in Aedes Aegypti and CYP612 overexpressed in Aedes Albopictus. The genetic mutation in A302S in Aedes Albopictus was associated with organochlorine resistance in Malaysia. Conclusions: Rotation of insecticide, integration with synergist and routine assessment of resistance profile are recom- mended strategies in insecticide resistance management. Keywords: Insecticide resistance; vector management; Aedes; pyrethroid; mortality.

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