scholarly journals Long-term warming does not affect soil ecoenzyme activity and original microbial nutrient limitation on the Qinghai—Tibet Plateau

Author(s):  
Yuanze Li ◽  
Huakun Zhou ◽  
Wenjing Chen ◽  
Yang Wu ◽  
LeiLei Qiao ◽  
...  
2021 ◽  
Author(s):  
Wenjing Chen ◽  
Huakun Zhou ◽  
Leilei Qiao ◽  
Yuanze Li ◽  
Yang Wu ◽  
...  

Abstract Background and aims Global warming has increasingly serious impacts on the structure and function of the Tibetan Plateau ecosystem. However, the mechanism by which warming affects the biogeochemical processes, and consequently the microbial nutrient limitation in soil aggregates, is not clear. Methods In the present study, we used open-top chamber experiments to simulate warming in an alpine meadow and an alpine shrubland on the Qinghai-Tibet Plateau, and we measured the C, N, and P-acquiring enzyme (β-1, 4-glucosidase, BG; leucine aminopeptidase, LAP; β-N-acetylglucosaminidase, NAG; alkali phosphatase, AP) activities and their stoichiometry to understand how warming affects microorganism-limiting mechanisms in soil aggregates. Results The results showed that long-term warming treatment significantly decreased soil organic carbon (SOC) and total nitrogen (TN) concentrations of large macroaggregates (LMGA) and small macroaggregates (SMGA) in alpine meadows, but significantly increased SOC concentration of LMGA in alpine shrubland. The SOC and TN concentrations of alpine meadows increased with the decrease of soil aggregate size and the concentrations in microaggregate (MIGA) were significantly higher than those LMGA. Soil enzyme activity increased with the decrease in aggregate size and was not significantly affected by warming treatment. Enzyme stoichiometry results demonstrated that soil microbes in alpine meadows and shrubland were limited by nutrient P relative to nitrogen; moreover, the long-term warming treatment aggravated the P limitation of soil microorganisms in the shrubland, and it had significant differences in LMGA and MIGA. At the same time, the long-term warming treatment had no significant effect on C limitation in the alpine shrubland and alpine meadows, but soil aggregate size affected the C limitation patterns of microorganisms and showed the greatest limitations in MIGA. Conclusions The microbial P limitation in shrubland is more sensitive to warming than that in meadow. Soil aggregates mediate the acquisition of C by microorganisms, and the C limitation in MIGA is the greatest. By providing a new perspective on this topic, our study increased our understanding of the effects of warming on microbial nutrient utilization and restriction patterns in soil aggregates.


2018 ◽  
Vol 19 (3) ◽  
pp. 1109-1119 ◽  
Author(s):  
Xiaolei Sun ◽  
Meng Li ◽  
Guoxi Wang ◽  
Marios Drosos ◽  
Fulai Liu ◽  
...  

2021 ◽  
Vol 290 ◽  
pp. 03001
Author(s):  
Qiang Peng ◽  
Yongjuan Sun

Magnetic susceptibility is an important means to quickly and economically monitor the enrichment of surrounding environmental elements. As a regional central city on the Qinghai-Tibet Plateau, the relationship between atmospheric dust magnetic susceptibility and heavy metal content in Xining area is relatively lacking. In this paper, the magnetic susceptibility and geochemical element content of the collected dust samples, loess samples, surface soil samples and soil profile samples obtained through long-term observations are analyzed. The results show that the magnetic susceptibility of the dust-fall samples is the highest, the soil samples are the second, and the loess samples are the lowest; the magnetic susceptibility of the dust-fall samples in Xining City has a significant correlation with the elements Cd, Cr, Ni, Cu, Pb, Zn and Hg, and the magnetic susceptibility can be used as an indicator of heavy metal in the soil; the elements Cr, Ni, Cu, Zn and As in the soil profile are enriched at 20 cm or 30 cm, which is related to the leaching of surface elements.


2019 ◽  
Vol 5 (6) ◽  
pp. 1227-1234 ◽  
Author(s):  
Xuejun Chen ◽  
Lei Wang ◽  
Zhikui Liu ◽  
Yinghong Qin

The construction of roadways in permafrost regions modifies ground-surface conditions and consequently, negatively varies thermal stability of the underlying frozen soils. To avoid the thawing of the permafrost layer under the scenario of global warming, roadways are usually laid on a built-up embankment, which not only disperses the traffic loads to underlying layers but also minimize the thermal disturbance. In the embankment, duct ventilation, or called air duct, can be embedded to further cool the underlying permafrost. While the thermal performance of duct ventilations has been well documented, the long-term structural stability of duct ventilation remains unknown. This study examines the structural stress of ventilation ducts that are placed in harsh weather such as the Qinghai-Tibet Plateau. The ducts are currently buried in the embankment filler, with the wind-outlet and -inlet ends exposed and cantilevered out of the embankment. Field studies found that the exposed parts have plagued cracking and even failures, especially at the fixed end of the cantilevered part. Damages of these concrete ducts are attributed to cyclic freezing-thawing attack, thermally-induced stresses, moisture-induced stresses, and concrete swelling. These physical attacks are caused by the harsh weather in the Qinghai-Tibet plateau. It is recommended to insulate the exposed part of the ducts and to fabricate durable and dense concrete ducts.


2021 ◽  
Vol 12 ◽  
Author(s):  
Yan Wang ◽  
Xin-Yu Li ◽  
Cai-Xia Li ◽  
Yuan He ◽  
Xin-Yi Hou ◽  
...  

Poa crymophila Keng is highly adaptable to long-term low temperature and drought conditions, making it a desirable foraging grass of the Qinghai-Tibet Plateau. Here, the widely targeted metabolomics and comparative transcriptome analyses were utilized for the discovery of metabolites and genes in P. crymophila in response to cold and drought stresses. P. crymophila were exposed to −5°C for 24 h and recovered to 22°C for 48 h, as well as drought for 10 days followed by re-watering for 1 day. In total, 779 metabolic features were assigned to metabolites and 167,845 unigenes were generated. Seventeen compounds showed significant up-regulation (variable importance in project >1) under both stresses in the metabolic profiling, mainly annotated as carbohydrates, flavones, and phenylpropanoids. The genes which were positively correlated with these metabolites were assigned to pathways (sucrose-starch, raffinose, phenylpropanoid, and flavone metabolism) using the Mapman software package. Alpha-amylase, beta-fructofuranosidase, and sugar transport genes degraded the glucose and starch to small molecule sugars for the purpose of osmotic adjustment and to provide more energy for the growth of P. crymophila in an adverse environment. The induction of cinnamoyl-CoA reductase (CCR) and the MYB gene as well as the sharp increase in schizandrin, a kind of lignan, showed that this likely has the closest connection with the tolerance to both stresses. Four significantly induced flavone compounds are probably involved in reducing oxidative damage. Our results indicated that activation of the phenlypropanoid pathway plays the primary role in P. crymophila adapting to harsh environments. This study showed the mechanism of P. crymophila responding to both cold and drought stresses and showed the discovery of a new biological regulator against stresses.


2021 ◽  
Author(s):  
Wenjing Chen ◽  
Huakun Zhou ◽  
Leilei Qiao ◽  
Yuanze Li ◽  
Yang Wu ◽  
...  

Abstract Background and aims Global warming has increasingly serious impacts on the structure and function of the Tibetan Plateau ecosystem. However, the mechanism by which warming affects the biogeochemical processes and consequently the microbial nutrient limitation in soil aggregates is not clear. Methods In the present study, we used open-top chamber experiments to simulate warming in an alpine meadow and an alpine shrubland on the Qinghai-Tibet Plateau to understand how warming affects nutrient utilization and microorganism-limiting mechanisms in soil aggregates. Results The results showed that long-term warming treatment had contrasting effects on soil organic carbon (SOC) content of the alpine meadow and that of the shrubland. This difference was more pronounced with the increase in soil aggregate size, and the SOC content in microaggregates (MIGA) was significantly higher than that in large macroaggregates (LMGA). Soil enzyme activity increased with the decrease in aggregate size and was not significantly affected by warming treatment. Enzyme stoichiometry demonstrated that microbial P limitation is widespread on the Tibetan Plateau, and the long-term warming treatment exacerbated it, which has significant differences in shrubland. At the same time, the long-term warming treatment had no significant effect on C limitation in the alpine shrubland and the alpine meadow, but soil aggregate size affected the C limitation patterns of microorganisms and showed strong limitations in MIGA. Conclusions The microbial P limitation in shrubland is more sensitive to warming than that of grassland. Soil aggregates mediate the acquisition of carbon by microorganisms, and the carbon limitation in MIGA is the greatest. By providing a new perspective on this topic, our study increased our understanding of the effects of warming on microbial nutrient utilization and restriction patterns in soil aggregates.


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