Chlorpromazine affects the numbers of Sox-2, Musashi1 and DCX-expressing cells in the rat brain subventricular zone

Abstract Background Adult neurogenesis observed both in the subventricular zone (SVZ) and hippocampus may be regulated and modulated by several endogenous factors, xenobiotics and medications. Classical and atypical antipsychotic drugs are able to affect neuronal and glial cell proliferation in the rat brain. The main purpose of this structural study was to determine whether chronic chlorpromazine treatment affects adult neurogenesis in the canonical sites of the rat brain. At present, the clinical application of chlorpromazine is rather limited; however, it may still represent an important model in basic neuropharmacological and toxicological studies. Methods The number of neural progenitors and immature neurons was enumerated using immunofluorescent detection of Sox2, Musashi1 and doublecortin (DCX) expression within SVZ. Results Chlorpromazine has a depressive effect on the early phase of adult neurogenesis in the rat subventricular zone (SVZ), as the mean number of Sox-2 immunoexpressing cells decreased following treatment. Conclusion Collectively, these results may suggest that long-term treatment with chlorpromazine may decrease neurogenic stem/progenitor cell formation in the rat SVZ and may affect rostral migratory stream formation.

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Long-term Treatment with Olanzapine Increases the Number of Sox2 and Doublecortin Expressing Cells in the Adult Subventricular Zone

CNS & Neurological Disorders - Drug Targets ◽

10.2174/1871527317666180627113544 ◽

2018 ◽

Vol 17 (6) ◽

pp. 458-463 ◽

Cited By ~ 2

Author(s):

Barbara Lasut ◽

Artur Palasz ◽

Lukasz Filipczyk ◽

Oscar Arias-Carrion ◽

Ewa Rojczyk ◽

...

Keyword(s):

Subventricular Zone ◽

Cell Formation ◽

Term Treatment ◽

Adult Brain ◽

Mood Stabilizers ◽

Fluorescent Detection ◽

Subgranular Zone ◽

Adult Rat ◽

Long Term Treatment

Background & Objective: Continuously active neurogenic regions in the adult brain are located in the subventricular zone (SVZ) of the lateral ventricles and subgranular zone of the hippocampal dentate gyrus. Neurogenesis is modulated by many factors such as growth factors, neurotransmitters and hormones. Neuropsychiatric drugs, especially antidepressants, mood stabilizers and antipsychotics may also affect the origin of neuronal cells. Method: The purpose of this study was to determine the effects of chronic olanzapine treatment on adult rat neurogenesis at the level of the SVZ. The number of neuroblasts was evaluated using immunohistochemical and fluorescent detection of sex determining region Y-box 2 and doublecortin expressing cells. Results & Conclusion: The results indicate that olanzapine has proneurogenic effects on the adult rat SVZ, as the mean number of sex determining region Y-box 2 and doublecortin-positive cells increased significantly, while there was a similar tendency in the subgranular zone. Collectively, these results suggest that long-term treatment with olanzapine may stimulate neurogenic stem cell formation in the SVZ which supports adult neurogenesis.

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