Direct determination of distribution volume and disappearance rate of native creatine kinase in humans

1983 ◽  
Vol 105 (3) ◽  
pp. 402-407 ◽  
Author(s):  
Giorgio Tommasini ◽  
Ronald P. Karlsberg ◽  
Franco Tamagni ◽  
Rinaldo Berra ◽  
Alessandro Oddone ◽  
...  
Keyword(s):  
Creatine Kinase ◽  
Direct Determination ◽  
Distribution Volume ◽  
Disappearance Rate

Direct measurement of creatine kinase-MB activity in serum after extraction with a monoclonal antibody specific to the MB isoenzyme.

1986 ◽  
Vol 32 (4) ◽  
pp. 657-663 ◽  
Author(s):  
H C Vaidya ◽  
Y Maynard ◽  
D N Dietzler ◽  
J H Ladenson
Keyword(s):  
Monoclonal Antibody ◽  
Creatine Kinase ◽  
Monoclonal Antibodies ◽  
Room Temperature ◽  
Direct Determination ◽  
Myeloma Cell ◽  
Myeloma Cell Line ◽  
Latex Beads ◽  
Creatine Kinase Mb

Abstract Fusion of splenocytes from A/J mice immunized by creatine kinase (EC 2.7.3.2)-MB isoenzyme (CK-MB) with SP2/0-Ag14 myeloma cell line generated hybridomas producing monoclonal antibodies specific to CK-MB. One of these monoclonal antibodies ("Conan-MB") was used to develop a direct assay for CK-MB activity. In the assay, serum is incubated for 30 min at room temperature with "Conan-MB" immobilized on latex beads. The beads are then washed, and CK-MB activity bound to the antibody is measured after incubation with CK enzyme reagent for 30 min at 37 degrees C. Results with the assay correlated well (r = 0.997) with those for CK-MB concentration as measured by a two-site immunoassay. Neither CK-MM, CK-BB, mitochondrial CK, nor a hemolysate of erythrocytes interfered. Use of this unique monoclonal antibody allows rapid, precise, and direct determination of CK-MB activity.

The Direct Determination of I131 in Heterogeneous Fecal Specimens

1961 ◽  
Vol 41 (4) ◽  
pp. 380-384 ◽  
Author(s):  
Arthur F. Dratz ◽  
James C. Coberly
Keyword(s):  
Direct Determination

A New Method for Direct Determination of the Recoilless Fraction with Application to Magnetic Nanoparticles

2002 ◽  
Vol 721 ◽  
Author(s):  
Monica Sorescu
Keyword(s):  
Room Temperature ◽  
Average Particle Size ◽  
Direct Determination ◽  
Average Particle ◽  
Lattice Method ◽  
Recoilless Fraction ◽  
Single Room ◽  
Magnetite Particles ◽  
Physical Mixtures

AbstractWe propose a two-lattice method for direct determination of the recoilless fraction using a single room-temperature transmission Mössbauer measurement. The method is first demonstrated for the case of iron and metallic glass two-foil system and is next generalized for the case of physical mixtures of two powders. We further apply this method to determine the recoilless fraction of hematite and magnetite particles. Finally, we provide direct measurement of the recoilless fraction in nanohematite and nanomagnetite with an average particle size of 19 nm.

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