Source input elucidation in aquatic systems by factor and principal component analysis of molecular marker data

1993 ◽  
Vol 278 (1) ◽  
pp. 159-176 ◽  
Author(s):  
Joan O. Grimalt ◽  
Joaquim Olivé
Keyword(s):  
Principal Component Analysis ◽  
Molecular Marker ◽  
Principal Component ◽  
Component Analysis ◽  
Aquatic Systems ◽  
Marker Data

scholarly journals Elucidation of Genetic Diversity as Morphological, Phytochemical and Molecular Markers in Some Hawthorn (Crataegus) Genotypes

2021 ◽  
Author(s):  
Ercan YILDIZ ◽  
Mehmet YAMAN ◽  
Ahmet SÜMBÜL ◽  
Ahmet SAY
Keyword(s):  
Genetic Diversity ◽  
Principal Component Analysis ◽  
Molecular Marker ◽  
Geographical Location ◽  
Similarity Index ◽  
Principal Component ◽  
Component Analysis ◽  
Biochemical Properties ◽  
Total Phenolic ◽  
Fruit Species

Abstract Backround: Thanks to its ecological and geographical location, Turkey is the homeland of many fruit species and allows many fruit species to be grown. Hawthorn, which is understood to be important in human health and nutrition, is one of these fruit types. This study was carried out to identify morphological, biochemical and molecular genetic variations of 22 hawthorn genotypes belonging to three different species collected from Kayseri province. Methods and Results: Morphological, biochemical and molecular marker (ISSR) techniques were used to determine genetic diversity. The fruit and leaf characteristics of the genotypes showed differences. Among the biochemical properties of the genotypes, the antioxidant activity ranged from 23.13–61.59%, the total flavonoid content ranged from 16.63 to 57.22 mg QE/100 g, and the total phenolic content ranged from 277.28 to 310.80 mg GAE/100 g. Genotypes were compared with principal component analysis according to their morphological and biochemical characteristics. In the principal component analysis, species generally formed similar clusters. In molecular marker analysis, 101 bands were obtained from 13 ISSR primers. 76 of the bands are polymorphic and the polymorphism rate was calculated as 75.24%. The similarity index in the UPGMA dendogram obtained as a result of the molecular analysis ranged between 0.71 and 0.88. In the dendrogram, genotypes did not show a dense clustering by species. Conclusion The results obtained may benefit researchers in the determination and protection of gene sources in breeding studies on hawthorn species.

A German version of the Intermittent Claudication Questionnaire (ICQ): cultural adaptation and validation

VASA ◽  
2012 ◽  
Vol 41 (5) ◽  
pp. 333-342 ◽  
Author(s):  
Kirchberger ◽  
Finger ◽  
Müller-Bühl
Keyword(s):  
Principal Component Analysis ◽  
Intermittent Claudication ◽  
Completion Time ◽  
Short Form ◽  
Principal Component ◽  
Component Analysis ◽  
German Version ◽  
Average Completion Time ◽  
Sf 36 ◽  
Related Quality

Background: The Intermittent Claudication Questionnaire (ICQ) is a short questionnaire for the assessment of health-related quality of life (HRQOL) in patients with intermittent claudication (IC). The objective of this study was to translate the ICQ into German and to investigate the psychometric properties of the German ICQ version in patients with IC. Patients and methods: The original English version was translated using a forward-backward method. The resulting German version was reviewed by the author of the original version and an experienced clinician. Finally, it was tested for clarity with 5 German patients with IC. A sample of 81 patients were administered the German ICQ. The sample consisted of 58.0 % male patients with a median age of 71 years and a median IC duration of 36 months. Test of feasibility included completeness of questionnaires, completion time, and ratings of clarity, length and relevance. Reliability was assessed through a retest in 13 patients at 14 days, and analysis of Cronbach’s alpha for internal consistency. Construct validity was investigated using principal component analysis. Concurrent validity was assessed by correlating the ICQ scores with the Short Form 36 Health Survey (SF-36) as well as clinical measures. Results: The ICQ was completely filled in by 73 subjects (90.1 %) with an average completion time of 6.3 minutes. Cronbach’s alpha coefficient reached 0.75. Intra-class correlation for test-retest reliability was r = 0.88. Principal component analysis resulted in a 3 factor solution. The first factor explained 51.5 of the total variation and all items had loadings of at least 0.65 on it. The ICQ was significantly associated with the SF-36 and treadmill-walking distances whereas no association was found for resting ABPI. Conclusions: The German version of the ICQ demonstrated good feasibility, satisfactory reliability and good validity. Responsiveness should be investigated in further validation studies.

Multifactorial analysis of clinical laboratory signs, the levels of IL-17A, IL-23, IL-33, IL-35, and specific antibodies in the serum of patients with Lyme borreliosis without erythema migrans

2020 ◽  
Vol 4 (11) ◽  
pp. 676-681
Author(s):  
V.V. Sapozhnikova ◽  
◽  
A.L. Bondarenko ◽  
Keyword(s):  
Principal Component Analysis ◽  
Lyme Borreliosis ◽  
Clinical Laboratory ◽  
Acute Infection ◽  
Erythema Migrans ◽  
Principal Component ◽  
Component Analysis ◽  
Control Group ◽  
Multifactorial Analysis ◽  
Specific Antibodies

Aim: to determine the association between clinical laboratory parameters, the production of cytokines (IL-17A, -23, -33, -35), and specific IgM and IgG in the serum of patients with Lyme borreliosis without erythema migrans. Patients and Methods: complete blood count, the concentrations of IL-17A, -23, -33, -35, and the levels of specific IgM and IgG were measured during acute infection and convalescence (n=30). The control group included age- and sex-matched healthy individuals (n=30). Statistical analysis was performed using the StatSoft Statistica v 10.0 software (parametric and non-parametric methods and multifactorial analysis, i.e., principal component analysis). Results: most (80%) patients with Lyme borreliosis without erythema migrans are the people of working age. In most patients, the combination of the specific antibodies against Borrelia afzelii and Borrelia garinii (76.7%) and severe intoxication and inflammatory process (100%) were detected. Moderate and severe disease associated with meningism was diagnosed in 90% and 10%, respectively. The mean duration of hectic period was 8.3±1.27 days. Abnormal ECG was reported in 40% of patients, i.e., conduction abnormalities in 20%, sinus bradycardia in 16.7%,and sinus tachycardia in 3.3%. The clinical laboratory signs of hepatitis without jaundice were identified in 26.7%. During treatment, the significant reduction in band and segmented neutrophil counts as well as the significant increase in platelet count were revealed compared to these parameters at admission. Abnormal cytokine levels (i.e., the increase in IL-17A, -23, -33 and the deficiency of IL-35) were detected. Conclusions: multifactorial analysis has demonstrated that the severity of immunological abnormalities in patients with Lyme borreliosis without erythema migrans is associated with fever, cardiac and liver disorders, the high levels of IL-23 and IL-33, and the lack of IL-35 and specific IgM and IgG. KEYWORDS: tick-borne borreliosis, Lyme disease without erythema migrans, clinical laboratory signs, cytokines, specific antibodies, multifactorial analysis, principal component analysis. FOR CITATION: Sapozhnikova V.V., Bondarenko A.L. Multifactorial analysis of clinical laboratory signs, the levels of IL-17A, IL-23, IL-33, IL-35, and specific antibodies in the serum of patients with Lyme borreliosis without erythema migrans. Russian Medical Inquiry. 2020;4(11):676–681. DOI: 10.32364/2587-6821-2020-4-11-676-681.

Oxypropylation of Brazilian Pine-Fruit Shell Evaluated by Principal Component Analysis

2018 ◽  
Vol 6 (7) ◽  
pp. 715-723 ◽  
Author(s):  
Stephany C. de Rezende ◽  
Jo鉶 A. Pinto ◽  
Isabel P. Fernandes ◽  
Fernanda V. Leimann and Maria-Filomena Barreiro
Keyword(s):  
Principal Component Analysis ◽  
Principal Component ◽  
Component Analysis
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