Application of selected ion monitoring technique for quantitative determination of picomole levels of pyrrolidine in the brain

The quantitative determination of glutamate is very important for diagnostic of many diseases of the nervous system, severity of stroke of the brain and also for determination glutamate in the food stuffs [1,2]. To the present days for quantitative determination of glutamate is wide used to the enzyme preparation of glutamate dehydrogenase (GDh) from bovine liver. However the application of GDh has the next serious disadvantages: high lability of this preparation and many substances strong change the activity of GDh. For example there are nucleotides, amino acid, steroid and metal ions. As the results the application of GDh preparation dont give reliable determination of quantity of glutamate. Thus there is the high necessity to propose another enzyme preparation for quantitative determination of glutamate without above mentioned disadvantages. In this reason we propose absolutely new enzyme preparation for this aim. In the laboratory of the enzyme structure and regulation of the Institute of the molecular biology and biochemistry of the Ministry of the education and science of the Republic of Kazakhstan was discovered the new enzyme complex (EC), which consists of malate dehydrogenase (MDh) and glutamate-oxaloacetate aminotransferase (GOAT) and used it for quantitative determination of glutamate concentration [3].

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Comparison of a New GLC-AFID Method With a GLC-MS Selected Ion Monitoring Technique and a Radioimmunoassay for the Determination of Plasma Concentrations of Imipramine and Desipramine

Journal of Analytical Toxicology ◽

10.1093/jat/4.5.237 ◽

1980 ◽

Vol 4 (5) ◽

pp. 237-243 ◽

Cited By ~ 7

Author(s):

K.K. Midha ◽

C. Charette ◽

J.K. Cooper ◽

I.J. McGilveray

Keyword(s):

Plasma Concentrations ◽

Selected Ion Monitoring ◽

Monitoring Technique

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New Internal Standard for Quantitative Determination of Oxyphytosterols by Gas Chromatography

Journal of AOAC International ◽

10.1093/jaoac/87.2.481 ◽

2004 ◽

Vol 87 (2) ◽

pp. 481-484 ◽

Cited By ~ 1

Author(s):

André Grandgirard ◽

Stéphanie Cabaret ◽

Lucy Martine ◽

Olivier Berdeaux

Keyword(s):

Mass Spectrometry ◽

Gas Chromatography ◽

Mass Spectrum ◽

Quantitative Determination ◽

Analytical Procedure ◽

Internal Standard ◽

Selected Ion Monitoring ◽

Electronic Impact ◽

Impact Mass

Abstract A study was conducted to develop a new internal standard for the quantitative determination of oxyphytosterols. Tests on 5α-androsten-3β,17β-diol; 5α-androstan-3β, 17β-diol; 5-pregnen-3β,20α-diol; and 5α-pregnan-3β,20β-diolshowed that these compounds were not fully adequate. However, the compound 3β,22-dihydroxy-20-homo-5-pregnene, synthesized in 4 steps, resulted in a promising internal standard, with a molecule similar to hydroxysterols; retention time as trimethylsilyl in gas chromatography comprised between 5α-cholestane and 7α-hydroxycholesterol; clear mass spectrum in electronic impact mass spectrometry, with several intense ions suitable for selected ion monitoring-mass spectrometry. Further studies are necessary to observe the behavior of these compounds during the entire analytical procedure.

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