The use of trinitrobenzenesulfonic acid in studies on the binding of fatty acid anions to bovine serum albumin

1971 ◽  
Vol 146 (2) ◽  
pp. 428-440 ◽  
Author(s):  
Lars-Olov Andersson ◽  
Johnny Brandt ◽  
Solveig Johansson
Keyword(s):  
Fatty Acid ◽  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Trinitrobenzenesulfonic Acid

scholarly journals Hypothyroidism in rats does not lower mitochondrial ADP/O and H+/O ratios

1988 ◽  
Vol 250 (2) ◽  
pp. 477-484 ◽  
Author(s):  
R P Hafner ◽  
M D Brand
Keyword(s):  
Fatty Acid ◽  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Adenylate Kinase ◽  
Bovine Serum ◽  
Mitochondrial Protein ◽  
Experimental Conditions ◽  
Low Concentrations ◽  
The Difference

We investigated reports that mitochondria isolated from hypothyroid rats have decreased ADP/O and H+/O ratios. We observed no decrease in the H+/O ratio in mitochondria from hypothyroid rats, in the presence of either 2% (w/v) fatty-acid-free bovine serum albumin or 100 nM free Ca2+. The ADP/O ratio in mitochondria isolated from hypothyroid rats in the presence of 2% fatty-acid-free bovine serum albumin was measured. Under normal experimental conditions we found no decrease in the ADP/O ratio, relative to that measured for littermate controls. At the low concentrations of mitochondrial protein used in the previously reported studies, the ADP/O ratio of mitochondria from hypothyroid rats was decreased, whereas that for control rats was only slightly decreased. The difference between the ADP/O ratios measured for mitochondria form hypothyroid rats and from control rats under these conditions was eliminated by inhibition of endogenous adenylate kinase. We suggest that the lowering of the apparent ADP/O ratio in mitochondria from hypothyroid rats at low concentrations of mitochondrial protein is an experimental artefact resulting from the breakdown of ADP to AMP.

Binding of some long-chain fatty acid anions and alcohols by bovine serum albumin

Biochemistry ◽  
1968 ◽  
Vol 7 (4) ◽  
pp. 1357-1361 ◽  
Author(s):  
Jacqueline A. Reynolds ◽  
Sarah. Herbert ◽  
Jacinto. Steinhardt
Keyword(s):  
Fatty Acid ◽  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Chain Fatty Acid ◽  
Long Chain Fatty Acid ◽  
Long Chain

Investigations of acetaminophen binding to bovine serum albumin in the presence of fatty acid: Fluorescence and 1H NMR studies

2009 ◽  
Vol 924-926 ◽  
pp. 332-337 ◽  
Author(s):  
B. Bojko ◽  
A. Sułkowska ◽  
M. Maciążek-Jurczyk ◽  
J. Równicka ◽  
W.W. Sułkowski
Keyword(s):  
Fatty Acid ◽  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
1H Nmr ◽  
Bovine Serum ◽  
Nmr Studies

Calcium ions reverse a latency period induced by bovine serum albumin in the time course of phospholipase A2 action on 1,2-dipalmitoyl-sn-glycero-3-phosphocholine liposomes

1993 ◽  
Vol 71 (9-10) ◽  
pp. 462-466 ◽  
Author(s):  
Fermín Pacheco ◽  
Ricardo Mejía ◽  
Marta S. Fernández
Keyword(s):  
Fatty Acid ◽  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Calcium Ions ◽  
Bovine Serum ◽  
Time Course ◽  
Latency Period ◽  
Phospholipase A ◽  
Ion Concentration ◽  
Lag Phase

The time course of hydrolysis of 1,2-dipalmitoyl-sn-glycero-3-phosphocholine liposomes in the gel phase catalyzed by porcine pancreatic phospholipase A2 was studied at 1 mM NaCl and variable CaCl2 concentrations, in the presence of delipidated bovine serum albumin. It has been found that the duration of the latency induced by bovine serum albumin shows an inverse dependence with CaCl2 concentration. As we showed previously, the induction of a lag phase by bovine serum albumin is related to its ability to sequester the fatty acid newly released by hydrolysis. Based on this and on our observation that there is an inverse dependence between the length of the latency period and the interfacial calcium ion concentration, it is interpreted that, while a direct effect of bovine serum albumin is the diminution of the liposome negative surface charge density by sequestration of the fatty acid released during hydrolysis, an indirect effect could be the decrease in the surface Ca2+ concentration. This, in turn, should diminish the enzyme binding to the lipid–water interface. The appearance of a latency phase seems to be the final consequence of these events.Key words: phospholipase A2, latency period, calcium ions, serum albumin.

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