Amino acid transport in a kidney epithelial cell line (MDCK): Characteristics of Na+/amino acid symport in membrane vesicles and basolateral localization in cell monolayers

1984 ◽  
Vol 234 (2) ◽  
pp. 330-340 ◽  
Author(s):  
Julia E. Lever ◽  
Brian G. Kennedy ◽  
Ranga Vasan
Keyword(s):  
Amino Acid ◽  
Epithelial Cell ◽  
Cell Line ◽  
Amino Acid Transport ◽  
Membrane Vesicles ◽  
Epithelial Cell Line ◽  
Acid Transport ◽  
Cell Monolayers ◽  
Kidney Epithelial Cell ◽  
Kidney Epithelial Cell Line

scholarly journals H+-gradient-dependent active transport of tetraethylammonium cation in apical-membrane vesicles isolated from kidney epithelial cell line LLC-PK1

1985 ◽  
Vol 227 (1) ◽  
pp. 199-203 ◽  
Author(s):  
K Inui ◽  
H Saito ◽  
R Hori
Keyword(s):  
Epithelial Cell ◽  
Cell Line ◽  
Apical Membrane ◽  
Membrane Vesicles ◽  
Epithelial Cell Line ◽  
Overshoot Phenomenon ◽  
Kidney Epithelial Cell ◽  
Kidney Epithelial Cell Line ◽  
Apical Membranes ◽  
Apical Membrane Vesicles

Transport of [3H]tetraethylammonium (NEt4+), an organic cation, has been studied by using apical-membrane vesicles isolated from cultured kidney epithelial cell line LLC-PK1. The uptake of NEt4+ by apical-membrane vesicles was osmotically sensitive, time-dependent and saturable. The presence of an H+ gradient ([H+]i greater than [H+]o) induced a marked stimulation of NEt4+ uptake against its concentration gradient (overshoot phenomenon), and this concentrative uptake was inhibited by HgCl2. These results suggest that apical membranes isolated from the LLC-PK1 cells retain the transport characteristics of NEt4+ similar to those observed in renal brush-border membranes.

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