Regulatory effects of insulin and experimental diabetes on neutral amino acid transport in the perfused rat exocrine pancreas. Kinetics of unidirectional l-serine influx and efflux at the basolateral plasma membrane

1984 ◽  
Vol 778 (3) ◽  
pp. 618-622 ◽  
Author(s):  
G.E. Mann ◽  
P.S.R. Norman
Keyword(s):  
Plasma Membrane ◽  
Amino Acid ◽  
Amino Acid Transport ◽  
Exocrine Pancreas ◽  
Experimental Diabetes ◽  
Acid Transport ◽  
Basolateral Plasma Membrane ◽  
Rat Exocrine Pancreas ◽  
Regulatory Effects ◽  
Kinetics Of

Amino acid transport in the rat exocrine pancreas

1977 ◽  
Vol 180 (1) ◽  
pp. 45-62 ◽  
Author(s):  
Wilfried Bieger ◽  
S. Peter ◽  
A. Völkl ◽  
H. F. Kern
Keyword(s):  
Amino Acid ◽  
Amino Acid Transport ◽  
Exocrine Pancreas ◽  
Acid Transport ◽  
Rat Exocrine Pancreas

Amino acid transport in the rat exocrine pancreas

1978 ◽  
Vol 194 (3) ◽  
Author(s):  
Guido Adler ◽  
Wilfried Bieger ◽  
HorstF. Kern
Keyword(s):  
Amino Acid ◽  
Amino Acid Transport ◽  
Exocrine Pancreas ◽  
Acid Transport ◽  
Rat Exocrine Pancreas

Amino acid transport in the rat exocrine pancreas

1977 ◽  
Vol 180 (1) ◽  
pp. 31-44 ◽  
Author(s):  
Wilfried Bieger ◽  
Stephan Peter ◽  
Horst F. Kern
Keyword(s):  
Amino Acid ◽  
Amino Acid Transport ◽  
Exocrine Pancreas ◽  
Acid Transport ◽  
Rat Exocrine Pancreas

scholarly journals Expression of solute carrier 7A4 (SLC7A4) in the plasma membrane is not sufficient to mediate amino acid transport activity

2002 ◽  
Vol 364 (3) ◽  
pp. 767-775 ◽  
Author(s):  
Sabine WOLF ◽  
Annette JANZEN ◽  
Nicole VÉKONY ◽  
Ursula MARTINÉ ◽  
Dennis STRAND ◽  
...  
Keyword(s):  
Plasma Membrane ◽  
Amino Acid ◽  
Protein Expression ◽  
Amino Acid Transport ◽  
Fluorescent Protein ◽  
Xenopus Laevis Oocytes ◽  
Amino Acid Transporters ◽  
Acid Transport ◽  
Transport Activity ◽  
Solute Carrier

Member 4 of human solute carrier family 7 (SLC7A4) exhibits significant sequence homology with the SLC7 subfamily of human cationic amino acid transporters (hCATs) [Sperandeo, Borsani, Incerti, Zollo, Rossi, Zuffardi, Castaldo, Taglialatela, Andria and Sebastio (1998) Genomics 49, 230–236]. It is therefore often referred to as hCAT-4 even though no convincing transport activity has been shown for this protein. We expressed SLC7A4 in Xenopus laevis oocytes, but could not detect any transport activity for cationic, neutral or anionic amino acids or for the polyamine putrescine. In addition, human glioblastoma cells stably overexpressing a fusion protein between SLC7A4 and the enhanced green fluorescent protein (EGFP) did not exhibit an increased transport activity for l-arginine. The lack of transport activity was not due to a lack of SLC7A4 protein expression in the plasma membrane, as in both cell types SLC7A4-EGFP exhibited a similar subcellular localization and level of protein expression as functional hCAT-EGFP proteins. The expression of SLC7A4 can be induced in NT2 teratocarcinoma cells by treatment with retinoic acid. However, also for this endogenously expressed SLC7A4, we could not detect any transport activity for l-arginine. Our data demonstrate that the expression of SLC7A4 in the plasma membrane is not sufficient to induce an amino acid transport activity in X. laevis oocytes or human cells. Therefore, SLC7A4 is either not an amino acid transporter or it needs additional (protein) factor(s) to be functional.

Kinetics of Neutral Amino Acid Transport Across the Blood-Brain Barrier

1987 ◽  
Vol 49 (5) ◽  
pp. 1651-1658 ◽  
Author(s):  
Quentin R. Smith ◽  
Seiji Momma ◽  
Masaki Aoyagi ◽  
Stanley I. Rapoport
Keyword(s):  
Amino Acid ◽  
Blood Brain Barrier ◽  
Amino Acid Transport ◽  
Neutral Amino Acid ◽  
Brain Barrier ◽  
Acid Transport ◽  
Blood Brain ◽  
Kinetics Of
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