Echinocytosis and microvesiculation of human erythrocytes induced by insertion of merocyanine 540 into the outer membrane leaflet

Long acyl chain sphingomyelin and saturated phospholipid tails in the outer membrane leaflet deplete cholesterol from the inner leaflet in mammalian membranes.

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Factors affecting the amount and the mode of merocyanine 540 binding to the membrane of human erythrocytes. A comparison with the binding to leukemia cells

Biochimica et Biophysica Acta (BBA) - Biomembranes ◽

10.1016/0005-2736(95)80032-b ◽

1995 ◽

Vol 1235 (2) ◽

pp. 428-436 ◽

Cited By ~ 33

Author(s):

Johan W.M. Lagerberg ◽

Karl-Josef Kallen ◽

Cees W.M. Haest ◽

John VanSteveninck ◽

Tom M.A.R. Dubbelman

Keyword(s):

Human Erythrocytes ◽

Leukemia Cells ◽

Factors Affecting ◽

Merocyanine 540

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Phosphatidylserine, a Lipid Present at the Outer Membrane Leaflet of Cancer Cells, Hinders the Insertion of pHLIP, a Potential Cancer Cell Marker

Biophysical Journal ◽

10.1016/j.bpj.2014.11.3027 ◽

2015 ◽

Vol 108 (2) ◽

pp. 552a

Author(s):

Haden Scott

Keyword(s):

Cancer Cells ◽

Outer Membrane ◽

Cancer Cell ◽

Cell Marker ◽

Potential Cancer ◽

Outer Membrane Leaflet

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The bovine seminal plasma protein PDC-109 extracts phosphorylcholine-containing lipids from the outer membrane leaflet

European Biophysics Journal ◽

10.1007/s00249-006-0105-3 ◽

2006 ◽

Vol 36 (4-5) ◽

pp. 461-475 ◽

Cited By ~ 15

Author(s):

Astrid Tannert ◽

Anke Kurz ◽

Karl-Rudolf Erlemann ◽

Karin Müller ◽

Andreas Herrmann ◽

...

Keyword(s):

Outer Membrane ◽

Plasma Protein ◽

Seminal Plasma ◽

Outer Membrane Leaflet

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Selective removal of lipids from the outer membrane layer of human erythrocytes without hemolysis. Consequences for bilayer stability and cell shape

Biochimica et Biophysica Acta (BBA) - Biomembranes ◽

10.1016/0005-2736(81)90174-7 ◽

1981 ◽

Vol 649 (3) ◽

pp. 701-708 ◽

Cited By ~ 46

Author(s):

C.W.M. Haest ◽

G. Plasa ◽

B. Deuticke

Keyword(s):

Outer Membrane ◽

Cell Shape ◽

Human Erythrocytes ◽

Selective Removal ◽

Membrane Layer ◽

Bilayer Stability

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Restricted diffusion of integral membrane proteins and polyphosphoinositides leads to their depletion in microvesicles released from human erythrocytes

Biochemical Journal ◽

10.1042/bj2710831 ◽

1990 ◽

Vol 271 (3) ◽

pp. 831-834 ◽

Cited By ~ 48

Author(s):

C Hagelberg ◽

D Allan

Keyword(s):

Lipid Bilayer ◽

Phospholipid Composition ◽

Human Erythrocytes ◽

Atp Depletion ◽

Ionophore A23187 ◽

Merocyanine 540 ◽

Dimyristoyl Phosphatidylcholine ◽

Normal Human ◽

Original Cell ◽

Membrane Components

The protein and phospholipid composition of microvesicles released from normal human erythrocytes after ATP depletion, on aging or by treatment with merocyanine 540, dimyristoyl phosphatidylcholine or Ca2+/ionophore A23187 has been compared with the composition of the original cell membrane. It has been shown that these microvesicles are depleted of band 3, glycophorin and phosphatidylinositol 4,5-bisphosphate relative to phospholipid by 40% or more. These data are interpreted to mean that less than half of these membrane components are free to diffuse laterally in the lipid bilayer. Acetylcholinesterase was found to be enriched 2-3-fold in microvesicles, possibly because the removal of non-diffusing proteins from the vesiculating region of the lipid bilayer allows more space for freely diffusing proteins like acetylcholinesterase to enter the microvesicle membrane.

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