An improved batchwise chromatographic procedure for creatine phosphokinase (CPK) isoenzyme separation

SUMMARY Since 1949 about 10,000 urinary corticoid analyses have been performed routinely in our laboratory. The method used for this purpose was described in 1950 (Sprechler). We determine the corticoids which can be extracted from the urine with chloroform immediately after acidification to pH 1. The extract is washed with sodium hydroxide and water, a Girard separation is performed, and finally the reducing power of the ketonic fraction is measured by means of the phosphomolybdic acid reagent reaction. During the last few years two other chemical reactions have been used for comparison: The formaldehyde and the Porter-Silber method. After a thorough examination of the above methods a standard technique was followed. In the formaldehyde method a microdiffusion in a Conway unit was used instead of distillation of the formaldehyde following the oxidation with periodic acid. The calibration curve was corrected for loss of material by taking the standard doses of DOC through all the procedures of the method. A micromodification of the Porter-Silber method was chosen. Furthermore attempts were made to determine how specific the chromatographic procedure is in the determination of steroids in urinary extracts. For this purpose the Florisil column was used, and the technique described by Nelson & Samuels was followed. Finally we have investigated the glucuronide-bound corticoids in urine in a smaller series of objects.

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Improved Cleanup and Derivatization for Gas Chromatographic Determination of Monosodium Glutamate in Foods

Journal of AOAC INTERNATIONAL ◽

10.1093/jaoac/66.6.1528 ◽

1983 ◽

Vol 66 (6) ◽

pp. 1528-1531 ◽

Cited By ~ 1

Author(s):

Hiroshi Nakanishi

Keyword(s):

Glutamic Acid ◽

Acid Derivative ◽

Monosodium Glutamate ◽

Chromatographic Determination ◽

Flame Ionization Detection ◽

Gas Chromatograph ◽

Flame Ionization ◽

Acetone Water ◽

Chromatographic Procedure

Abstract A gas chromatographic procedure is described for determining monosodium glutamate (MSG) in several types of food. A sample is extracted with acetone- water (1 + 1). Acetone is evaporated and an aliquot of the extract is buffered with 1M NH4OH-1M NH4CI pH 9 solution, and chromatographed directly on a column of QAE Sephadex A-25 that has been pretreated with the same buffer. MSG is eluted with 0.1N HC1, and a portion of the eluate is evaporated to dryness and reacted with dimethylformamide( DMF)-dimethylacetal to form the glutamic acid derivative, which is injected into a gas chromatograph and measured by flame ionization detection. Recoveries of MSG from sample fortified at 5-500 mg ranged from 92.8 to 100%.

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Coupled in vivo activity of creatine phosphokinase and the membrane-bound (Na+,K+)-ATPase in the resting and stimulated electric organ of the electric fish Narcine brasiliensis

Journal of Biological Chemistry ◽

10.1016/s0021-9258(18)99218-5 ◽

1991 ◽

Vol 266 (16) ◽

pp. 10254-10259

Author(s):

H. Blum ◽

J.A. Balschi ◽

R.G. Johnson

Keyword(s):

Electric Fish ◽

Creatine Phosphokinase ◽

Electric Organ ◽

Membrane Bound

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Eignung der Kapillarmikroskopie zur Diagnostik und Differenzierung einer ILD

Karger Kompass Pneumologie ◽

10.1159/000514899 ◽

2021 ◽

pp. 1-3

Author(s):

Sabine Adler

Keyword(s):

Computed Tomography ◽

Creatine Phosphokinase ◽

Correct Diagnosis ◽

Diagnostic Assessment ◽

Idiopathic Inflammatory Myopathies ◽

Inflammatory Myopathies ◽

Nailfold Videocapillaroscopy ◽

High Resolution Computed Tomography ◽

Diagnostic Role

Nailfold videocapillaroscopy (NVC) is an easy tool used for the assessment of patients with Raynaud’s phenomenon (RP) as possibly associated with systemic sclerosis (SSc). Recent insights have also highlighted its role in the diagnostic assessment of idiopathic inflammatory myopathies (IIMs). The aim of this study is to describe the diagnostic role of NVC in a series of 361 consecutive patients with interstitial lung disease (ILD). All the patients were assessed by clinical pulmonary and rheumatic examinations, blood exams, high-resolution computed tomography and NVC. NVC was considered positive only in the presence of avascular areas or giant capillaries, but also, the presence of bushy capillaries (BCs) was recorded. NVC was positive in 17.7% of ILD patients and in 78.1% of ILD patients associated with a diagnosis of connective tissue disease (CTD). In 25% of SSc-ILD patients, NVC proved necessary for a correct diagnosis. The presence of BCs and/or NVC positivity in ILD patients with normal levels of creatine phosphokinase is associated with amyopathic IIM, regardless the presence of RP. In conclusion, NVC is useful for the diagnostic assessment of incomplete forms of CTD and in amyopathic IIMs. NVC should be considered in the diagnostic assessment of ILD patients regardless of the presence of RP.

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One-step chromatographic procedure for purification of B-phycoerythrin from Porphyridium cruentum

Protein Expression and Purification ◽

10.1016/j.pep.2016.01.018 ◽

2016 ◽

Vol 123 ◽

pp. 70-74 ◽

Cited By ~ 20

Author(s):

Zhihong Tang ◽

Jilu zhao ◽

Bao Ju ◽

Wenjun Li ◽

Shaohong Wen ◽

...

Keyword(s):

Porphyridium Cruentum ◽

Chromatographic Procedure ◽

One Step

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Simultaneous liquid-chromatographic determination of vitamin K1 and vitamin E in serum.

Clinical Chemistry ◽

10.1093/clinchem/35.12.2285 ◽

1989 ◽

Vol 35 (12) ◽

pp. 2285-2289 ◽

Cited By ~ 13

Author(s):

B E Cham ◽

H P Roeser ◽

T W Kamst

Keyword(s):

Vitamin E ◽

Human Serum ◽

High Performance ◽

Chromatographic Determination ◽

High Performance Liquid Chromatographic ◽

Tocopheryl Acetate ◽

Vitamin K1 ◽

Chromatographic Procedure ◽

Liquid Chromatographic Determination ◽

Liquid Chromatographic

Abstract We describe a high-performance liquid chromatographic procedure for the simultaneous measurement of vitamins K1 and E in human serum. Delipidated human serum (free of vitamins K1 and E) was used to make standard solutions of these vitamins, and cetyl naphthoate and alpha-tocopheryl acetate were the internal standards for vitamin K1 and vitamin E, respectively. A simple, novel separation method utilizing liquid-liquid partition chromatography was used as a preparative "clean-up" procedure. Cetyl naphthoate and vitamin K1 (after post-column reduction) were detected by fluorescence, alpha-tocopheryl acetate and vitamin E by ultraviolet absorption. Sensitivity (detection limit) of the assay was 30 pg for vitamin K1 and 5 ng for vitamin E per injection. The method is specific, precise, and more rapid than previously described procedures. Within- and between-assay CVs were 8.1% and 12.9%, respectively, for vitamin K1; 3.5% and 6.0%, respectively, for vitamin E. Analytical recoveries of vitamins K1 and E were 80% and 93%, respectively, from serum and from delipidated serum (standards). The average neonatal serum concentration of vitamin K1 was 83 ng/L, 2.5 mg/L for vitamin E; for normolipidemic adults, the values were 343 ng/L and 7.9 mg/L, respectively, and for hyperlipidemic adults, 541 ng/L and 11.1 mg/L, respectively.

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