Improved Cleanup and Derivatization for Gas Chromatographic Determination of Monosodium Glutamate in Foods

1983 ◽  
Vol 66 (6) ◽  
pp. 1528-1531 ◽  
Author(s):  
Hiroshi Nakanishi
Keyword(s):  
Glutamic Acid ◽  
Acid Derivative ◽  
Monosodium Glutamate ◽  
Chromatographic Determination ◽  
Flame Ionization Detection ◽  
Gas Chromatograph ◽  
Flame Ionization ◽  
Acetone Water ◽  
Chromatographic Procedure

Abstract A gas chromatographic procedure is described for determining monosodium glutamate (MSG) in several types of food. A sample is extracted with acetone- water (1 + 1). Acetone is evaporated and an aliquot of the extract is buffered with 1M NH4OH-1M NH4CI pH 9 solution, and chromatographed directly on a column of QAE Sephadex A-25 that has been pretreated with the same buffer. MSG is eluted with 0.1N HC1, and a portion of the eluate is evaporated to dryness and reacted with dimethylformamide( DMF)-dimethylacetal to form the glutamic acid derivative, which is injected into a gas chromatograph and measured by flame ionization detection. Recoveries of MSG from sample fortified at 5-500 mg ranged from 92.8 to 100%.

Gas-chromatographic determination of cholesterol sulfate in plasma and erythrocytes, for the diagnosis of recessive X-linked ichthyosis.

1983 ◽  
Vol 29 (7) ◽  
pp. 1404-1407 ◽  
Author(s):  
F A Muskiet ◽  
G Jansen ◽  
B G Wolthers ◽  
A Marinkovic-Ilsen ◽  
P C van Voorst Vader
Keyword(s):  
Internal Standard ◽  
Dehydroepiandrosterone Sulfate ◽  
Chromatographic Determination ◽  
Anion Exchange Chromatography ◽  
Exchange Chromatography ◽  
Single Step ◽  
Flame Ionization Detection ◽  
Cholesterol Sulfate ◽  
Flame Ionization

Abstract We describe a rapid method for determining cholesterol sulfate in plasma and erythrocytes. After its single-step isolation by means of anion-exchange chromatography cholesterol sulfate is hydrolyzed, trimethylsilylated, and determined by gas chromatography with flame ionization detection. 5 beta-Cholestan-3 alpha-ol sulfate is used as internal standard. The method enables simultaneous determination of dehydroepiandrosterone sulfate in plasma. We applied it for the diagnosis of seven patients with recessive X-linked ichthyosis. Concentrations are given for plasma and erythrocytes from four unaffected relatives of patients with X-linked ichthyosis, a patient with placental sulfatase deficiency, two patients with other types of ichthyoses, and 20 controls. The method may also be of use for the rapid isolation of other organic sulfates from biological material, as illustrated by a comparison of gas chromatograms of urine from a normal pregnant woman and that from a patient with placental sulfatase deficiency.

Direct Blood-Injection Method for Gas Chromatographic Determination of Alcohols and Other Volatile Compounds

1971 ◽  
Vol 17 (2) ◽  
pp. 82-85 ◽  
Author(s):  
Naresh C Jain
Keyword(s):  
Sample Preparation ◽  
Volatile Compounds ◽  
Low Cost ◽  
Internal Standard ◽  
Chromatographic Determination ◽  
Gas Chromatograph ◽  
Ionization Detector ◽  
Flame Ionization ◽  
Blood Injection

Abstract An extremely simple, rapid method is described for simultaneously determining methanol, ethanol, acetone, isopropanol, and low-boiling hydrocarbons associated with glue sniffing. Less than 1 µl of blood, mixed with an internal standard, is injected directly into a low-cost gas chromatograph equipped with a flame-ionization detector. No extraction, distillation, and (or) sample preparation is required, and the method is sensitive to less than 10 µg of alcohol per ml.

Rapid High Performance Liquid Chromatographic Determination of Monosodium Glutamate in Food

1982 ◽  
Vol 65 (3) ◽  
pp. 567-571
Author(s):  
Peter Sporns
Keyword(s):  
Glutamic Acid ◽  
High Performance ◽  
Monosodium Glutamate ◽  
Chromatographic Determination ◽  
High Performance Liquid Chromatographic ◽  
Soy Sauce ◽  
Liquid Chromatographic Method ◽  
Liquid Chromatographic Determination ◽  
Liquid Chromatographic

Abstract A rapid, accurate high performance liquid chromatographic method is described for determination of glutamic acid in food. Average recovery of added glutamic acid was 99.2% by this method. The method could be used to analyze samples such as soy sauce, which contain a large amount of other potentially interfering soluble compounds.

Gas Chromatographic Determination of Ethanol and Isopropanol or Acetone in Drugs

1973 ◽  
Vol 56 (3) ◽  
pp. 684-688
Author(s):  
Nicholas Falcone
Keyword(s):  
Direct Injection ◽  
Chromatographic Determination ◽  
Pharmaceutical Products ◽  
Chromatographic Technique ◽  
Flame Ionization Detection ◽  
Commercial Products ◽  
Porapak Q ◽  
Flame Ionization ◽  
Concentration Levels

Abstract A gas-solid chromatographic technique, using flame ionization detection and a Porapak Q column, is presented for the identification and determination of ethanol and isopropanol or acetone in pharmaceutical products. The technique involves direct injection of an aqueous dilution of the product and is therefore simple and direct. The commercial products and 3 simulated mixtures, at various concentration levels, were analyzed by 9 collaborators. Recoveries and standard deviations for all 3 components were satisfactory. The method has been adopted as official first action.

Gas-Liquid Chromatographic Determination of Sorbitol in Cooked Sausage Products

1978 ◽  
Vol 61 (1) ◽  
pp. 164-166
Author(s):  
Fred A Moseley ◽  
Joel S Salinsky ◽  
Robert W Woods
Keyword(s):  
Chromatographic Determination ◽  
Flame Ionization Detector ◽  
Gas Chromatograph ◽  
Ionization Detector ◽  
Simple Method ◽  
Flame Ionization ◽  
Freeze Dried ◽  
Liquid Chromatographic Determination ◽  
Liquid Chromatographic

Abstract A rapid and simple method of analysis has been developed for sorbitol in cooked sausage products. Sorbitol is extracted from cooked sausage products with water, an aliquot of the extract is freeze-dried, and the trimethylsilyl (TMS) derivative of sorbitol is formed. An aliquot of the TMS-sorbitol is injected into a gas chromatograph and measured by a flame ionization detector. Analysis of fortified samples shows that the recovery compares well with known amounts of sorbitol added.

scholarly journals Liquid Chromatographic Determination of Sugar Alcohols in Beverages and Foods after Nitrobenzoylation

1999 ◽  
Vol 82 (1) ◽  
pp. 134-140 ◽  
Author(s):  
Shuko Nojiri ◽  
Kazuo Saito ◽  
Nobuo Taguchi ◽  
Mitsuo Oishi ◽  
Toshio Maki
Keyword(s):  
Mobile Phase ◽  
Chromatographic Determination ◽  
Flame Ionization Detection ◽  
Sugar Alcohols ◽  
Flame Ionization ◽  
Phase Calibration ◽  
Liquid Chromatographic Determination ◽  
Liquid Chromatographic ◽  
Refractive Index Detection

Abstract Use of p-nitrobenzoyl chloride (PNBC) to form an ultraviolet-absorbing derivative was attempted to determine the sugar alcohols meso-erythritol, xyli-tol, D-sorbitol,and D-mannitol by liquid chromatography (LC). LC determination of derivatives was performed on an ODS column with acetonitrile–water (65 + 35) as mobile phase. Calibration curves were linear in the concentration range 0.01–100 μg/mL. Method sensitivity is 10 to 1000 times higher than that of LC with refractive index detection and gas chromatography with flame ionization detection. Recoveries of sugars added to various foods at 0.1 and 1% ranged from 91 to 102% for meso-erythritol, 75 to 115% for xylitol, 81 to 105% for D-sorbitol, and 81 to 108% for D-mannitol.

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