Phospholipid content of subcellular fractions in adult rat organs

1961 ◽  
Vol 51 (2) ◽  
pp. 221-226 ◽  
Author(s):  
J.J. Biezenski ◽  
Theodore H. Spaet
1963 ◽  
Vol 70 ◽  
pp. 75-84 ◽  
Author(s):  
J.J. Biezenski ◽  
T.H. Spaet ◽  
A.L. Gordon

Author(s):  
Fukushi Kambe ◽  
Shin'ichiro Tsukahara ◽  
Taichi Kato ◽  
Hisao Seo

1969 ◽  
Vol 115 (5) ◽  
pp. 1051-1062 ◽  
Author(s):  
N. L. Banik ◽  
A. N. Davison

1. Subcellular fractions and myelin were isolated from developing and adult rat brain. 2. Measurements of chemical composition and enzyme activities indicate the presence of a second myelin-like fraction mainly in the brain of developing rats. 3. This membrane fraction has a different lipid composition from myelin, but resembles myelin in its content of phosphohydrolase and aminopeptidase activity. 4. It is suggested that the second myelin-like fraction may be a submicrosomal contaminant or it may be derived from oligodendroglial plasma membrane during myelinogenesis.


1976 ◽  
Vol 69 (3) ◽  
pp. 592-598 ◽  
Author(s):  
Zelina Leibovitz-Ben Gershon ◽  
Shimon Gatt

1986 ◽  
Vol 60 (5) ◽  
pp. 1665-1672 ◽  
Author(s):  
S. L. Young ◽  
R. Silbajoris

Prenatal administration of glucocorticoids stimulates epithelial cell maturation and induces a precocious development of pulmonary surfactant. The response of the adult lung to steroid administration is less well understood. We administered dexamethasone (2 mg X kg-1 X day-1) to adult male rats for 1 wk by daily subcutaneous injection. After pentobarbital anesthesia we lavaged the lungs and also isolated lamellar bodies from the tissue. Lipid analyses of the extracellular and intracellular surfactant compartments showed two- to fourfold greater amounts of total phospholipids and disaturated phosphatidylcholine compared with control. These changes were not found in kidney nor liver and were not present in plasma membrane, mitochondrial, or microsomal fractions from lungs. Morphometric analyses of the type II cells showed that anatomic measures of the lamellar body pool did not increase. We conclude that glucocorticoids have a significant effect to increase lung surfactant lipid pools of adult rat lungs by changing the phospholipid content of lamellar bodies, without changing lamellar body volume.


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