Early events of chemical transmission of newly formed neuromuscular junctions in monolayers of human muscle cells co-cultured with fetal rat spinal cord explants

1991 ◽  
Vol 538 (1) ◽  
pp. 79-85 ◽  
Author(s):  
Makoto Michikawa ◽  
Takayoshi Kobayashi ◽  
Hiroshi Tsukagoshi
Keyword(s):  
Spinal Cord ◽  
Neuromuscular Junctions ◽  
Muscle Cells ◽  
Human Muscle ◽  
Rat Spinal Cord ◽  
Fetal Rat ◽  
Human Muscle Cells ◽  
Chemical Transmission

Neural agrin controls maturation of the excitation-contraction coupling mechanism in human myotubes developing in vitro

2008 ◽  
Vol 294 (1) ◽  
pp. C66-C73 ◽  
Author(s):  
Elena Bandi ◽  
Marko Jevšek ◽  
Tomaz Mars ◽  
Mihaela Jurdana ◽  
Elena Formaggio ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Spinal Cord ◽  
Muscle Cells ◽  
Human Muscle ◽  
Coupling Mechanism ◽  
Rat Spinal Cord ◽  
Excitation Contraction Coupling ◽  
Contraction Coupling ◽  
Human Myotubes ◽  
Human Muscle Cells

The aim of this study was to elucidate the mechanisms responsible for the effects of innervation on the maturation of excitation-contraction coupling apparatus in human skeletal muscle. For this purpose, we compared the establishment of the excitation-contraction coupling mechanism in myotubes differentiated in four different experimental paradigms: 1) aneurally cultured, 2) cocultured with fetal rat spinal cord explants, 3) aneurally cultured in medium conditioned by cocultures, and 4) aneurally cultured in medium supplemented with purified recombinant chick neural agrin. Ca2+ imaging indicated that coculturing human muscle cells with rat spinal cord explants increased the fraction of cells showing a functional excitation-contraction coupling mechanism. The effect of spinal cord explants was mimicked by treatment with medium conditioned by cocultures or by addition of 1 nM of recombinant neural agrin to the medium. The treatment with neural agrin increased the number of human muscle cells in which functional ryanodine receptors (RyRs) and dihydropyridine-sensitive L-type Ca2+ channels were detectable. Our data are consistent with the hypothesis that agrin, released from neurons, controls the maturation of the excitation-contraction coupling mechanism and that this effect is due to modulation of both RyRs and L-type Ca2+ channels. Thus, a novel role for neural agrin in skeletal muscle maturation is proposed.

Histoenzymatic profile of human muscle cultured in monolayer and innervated de novo by fetal rat spinal cord

1988 ◽  
Vol 11 (1) ◽  
pp. 1-9 ◽  
Author(s):  
Giuseppe Vita ◽  
Valerie Askanas ◽  
Andrea Martinuzzi ◽  
W. King Engel
Keyword(s):  
Spinal Cord ◽  
De Novo ◽  
Human Muscle ◽  
Rat Spinal Cord ◽  
Fetal Rat

Differentiation of glial cells and motor neurons during the formation of neuromuscular junctions in cocultures of rat spinal cord explant and human muscle

2001 ◽  
Vol 438 (2) ◽  
pp. 239-251 ◽  
Author(s):  
Tomaz Mars ◽  
Kevin J. Yu ◽  
Xue-Ming Tang ◽  
Armand F. Miranda ◽  
Zoran Grubic ◽  
...  
Keyword(s):  
Spinal Cord ◽  
Glial Cells ◽  
Motor Neurons ◽  
Neuromuscular Junctions ◽  
Human Muscle ◽  
Rat Spinal Cord

Developmental study of the expression of dystrophin in cultured human muscle aneurally and innervated with fetal rat spinal cord

1991 ◽  
Vol 565 (2) ◽  
pp. 280-289 ◽  
Author(s):  
Yong Choo Park-Matsumoto ◽  
Noriyoshi Kameda ◽  
Takayoshi Kobayashi ◽  
Hiroshi Tsukagoshi
Keyword(s):  
Spinal Cord ◽  
Human Muscle ◽  
Developmental Study ◽  
Rat Spinal Cord ◽  
Fetal Rat

The effect of Ba ions on human muscle cultured in monolayer and innervated with fetal rat spinal cord

1990 ◽  
Vol 111 (1-2) ◽  
pp. 157-163 ◽  
Author(s):  
Takayoshi Kobayashi ◽  
Makoto Michikawa ◽  
Hiroshi Miyazaki ◽  
Hiroshi Tsukagoshi
Keyword(s):  
Spinal Cord ◽  
Human Muscle ◽  
Rat Spinal Cord ◽  
Fetal Rat
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