Background:
The need for suitable antibacterial agents effective against Multi-drug
resistant Gram-negative bacteria is acknowledged globally. The present study was designed to
evaluate the possible antibacterial potential of an extracted compound from edible flowers of
Moringa oleifera.
Methods:
Five different solvents were used for preparing dried flower extracts. The most effective
extract was subjected to fractionation and further isolation of the active compound with the
highest antibacterial effect was obtained using TLC, Column Chromatography and reverse phase-
HPLC. Approaches were made for characterization of the isolated compound using FTIR, NMR
and Mass spectrometry. Antibacterial activity was evaluated according to the CLSI guidelines.
Results:
One fraction of aqueous acetic acid extract of M. oleifera flower was found highly
effective and more potent than conventional antibiotics of different classes against Multi-drug resistant
Gram-negative bacilli (MDR-GNB) when compared. The phytochemical analysis of the
isolated compound revealed the presence of hydrogen-bonded amine and hydroxyl groups
attributable to unsaturated amides.
Conclusion:
The present study provided data indicating a potential for use of the flowers extract
of M. oleifera in the fight against infections caused by lethal MDR-GNB.
Recommendations:
Aqueous acetic acid flower extract of M. oleifera is effective, in-vitro, against
Gram-negative bacilli. This finding may open a scope in pharmaceutics for the development of
new classes of antibiotics.
Oxidation of ℓ–Alanine and ℓ–Leucine by 1,3-Dichloro-5,5- Dimethylhydantoin in Aqueous Acetic Acid Medium : A Kinetic Study
